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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.8 P.897-899


Characteristics of photosynthesis in rice plants transformed with an antisense Rubisco activase gene

Author(s):  JIN Song-heng, JIANG De-an, LI Xue-qin, SUN Jun-wei

Affiliation(s):  State Key Lab of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   dajiang@cls.zju.edu.cn

Key Words:  Antisense rca rice plants, Rubisco, Rubisco activase, Photosynthesis

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JIN Song-heng, JIANG De-an, LI Xue-qin, SUN Jun-wei. Characteristics of photosynthesis in rice plants transformed with an antisense Rubisco activase gene[J]. Journal of Zhejiang University Science A, 2004, 5(8): 897-899.

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A1 - JIANG De-an
A1 - LI Xue-qin
A1 - SUN Jun-wei
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DOI - 10.1631/jzus.2004.0897

Transgenic rice plants with an antisense gene inserted via Agrobacterium tumefaciens were used to explore the impact of the reduction of rubisco activase (RCA) on rubisco and photosynthesis. In this study, transformants containing 15% to 35% wild type rubisco activase were selected, which could survive in ambient CO2 concentration but grew slowly compared with wild type controls. Gas exchange measurements indicated that the rate of photosynthesis decreased significantly, while stomatal conductance and transpiration rate did not change; and that the intercellular CO2 concentration even increased. rubisco determination showed that these plants had approximately twice as much rubisco as the wild types, although they showed 70% lower rate of photosynthesis, which was likely an acclimation response to the reduction in Rubsico activase and/or the reduction in carbamylation.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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