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Received: 2003-12-20

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.3 P.165-170


Molecular analysis of microbial community in a groundwater sample polluted by landfill leachate and seawater

Author(s):  TIAN Yang-jie, YANG Hong, WU Xiu-juan, LI Dao-tang

Affiliation(s):  School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China

Corresponding email(s):   hongyang@sjtu.edu.cn

Key Words:  Saline groundwater, Landfill, 16S rRNA, Clone library, Phylogenetic analysis

TIAN Yang-jie, YANG Hong, WU Xiu-juan, LI Dao-tang. Molecular analysis of microbial community in a groundwater sample polluted by landfill leachate and seawater[J]. Journal of Zhejiang University Science B, 2005, 6(3): 165-170.

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%T Molecular analysis of microbial community in a groundwater sample polluted by landfill leachate and seawater
%A TIAN Yang-jie
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T1 - Molecular analysis of microbial community in a groundwater sample polluted by landfill leachate and seawater
A1 - TIAN Yang-jie
A1 - YANG Hong
A1 - WU Xiu-juan
A1 - LI Dao-tang
J0 - Journal of Zhejiang University Science B
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2005.B0165

Seashore landfill aquifers are environments of special physicochemical conditions (high organic load and high salinity), and microbes in leachate-polluted aquifers play a significant role for intrinsic bioremediation. In order to characterize microbial diversity and look for clues on the relationship between microbial community structure and hydrochemistry, a culture-independent examination of a typical groundwater sample obtained from a seashore landfill was conducted by sequence analysis of 16S rDNA clone library. Two sets of universal 16S rDNA primers were used to amplify DNA extracted from the groundwater so that problems arising from primer efficiency and specificity could be reduced. Of 74 clones randomly selected from the libraries, 30 contained unique sequences whose analysis showed that the majority of them belonged to bacteria (95.9%), with Proteobacteria (63.5%) being the dominant division. One archaeal sequence and one eukaryotic sequence were found as well. Bacterial sequences belonging to the following phylogenic groups were identified: Bacteroidetes (20.3%), β, γ, δ and ε-subdivisions of Proteobacteria (47.3%, 9.5%, 5.4% and 1.3%, respectively), Firmicutes (1.4%), Actinobacteria (2.7%), Cyanobacteria (2.7%). The percentages of Proteobacteria and Bacteroides in seawater were greater than those in the groundwater from a non-seashore landfill, indicating a possible influence of seawater. Quite a few sequences had close relatives in marine or hypersaline environments. Many sequences showed affiliations with microbes involved in anaerobic fermentation. The remarkable abundance of sequences related to (per)chlorate-reducing bacteria (ClRB) in the groundwater was significant and worthy of further study.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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