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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.6 P.457-463

http://doi.org/10.1631/jzus.2005.B0457


Pathogenicity of bacterium, Xenorhabdus nematophila isolated from entomopathogenic nematode (Steinernema carpocapsae) and its secretion against Galleria mellonella larvae


Author(s):  MAHAR Ali Nawaz, MUNIR Muhammad, ELAWAD Sami, GOWEN Simon Richard, HAGUE Nigel Graham Meckenzi

Affiliation(s):  Department of Agriculture, University of Reading, Reading RG6 6AT, UK; more

Corresponding email(s):   m_munir66@yahoo.co.uk

Key Words:  Biological control, Bacterial symbionts, Entomopathogenic nematodes, Xenorhabdus nematophila, Galleria mellonella


MAHAR Ali Nawaz, MUNIR Muhammad, ELAWAD Sami, GOWEN Simon Richard, HAGUE Nigel Graham Meckenzi. Pathogenicity of bacterium, Xenorhabdus nematophila isolated from entomopathogenic nematode (Steinernema carpocapsae) and its secretion against Galleria mellonella larvae[J]. Journal of Zhejiang University Science B, 2005, 6(6): 457-463.

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author="MAHAR Ali Nawaz, MUNIR Muhammad, ELAWAD Sami, GOWEN Simon Richard, HAGUE Nigel Graham Meckenzi",
journal="Journal of Zhejiang University Science B",
volume="6",
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pages="457-463",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0457"
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%A MAHAR Ali Nawaz
%A MUNIR Muhammad
%A ELAWAD Sami
%A GOWEN Simon Richard
%A HAGUE Nigel Graham Meckenzi
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0457

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T1 - Pathogenicity of bacterium, Xenorhabdus nematophila isolated from entomopathogenic nematode (Steinernema carpocapsae) and its secretion against Galleria mellonella larvae
A1 - MAHAR Ali Nawaz
A1 - MUNIR Muhammad
A1 - ELAWAD Sami
A1 - GOWEN Simon Richard
A1 - HAGUE Nigel Graham Meckenzi
J0 - Journal of Zhejiang University Science B
VL - 6
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SP - 457
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%@ 1673-1581
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2005.B0457


Abstract: 
The entomopathogenic bacterium, Xenorhabdus nematophila was isolated from the hemolymph of Galleria mellonella infected with Steinernema carpocapsae. The bacterial cells and its metabolic secretions have been found lethal to the Galleria larvae. Toxic secretion in broth caused 95% mortality within 4 d of application whereas the bacterial cells caused 93% mortality after 6 d. When filter and sand substrates were compared, the later one was observed as appropriate. Similarly, bacterial cells and secretion in broth were more effective at 14% moisture and 25 °C temperature treatments. Maximum insect mortality (100%) was observed when bacterial concentration of 4×106 cells/ml was used. Similarly, maximum bacterial cells in broth (95%) were penetrated into the insect body within 2 h of their application. However, when stored bacterial toxic secretion was applied to the insects its efficacy declined. On the other hand, when the same toxic secretion was dried and then dissolved either in broth or water was proved to be effective. The present study showed that the bacterium, X. nematophila or its toxic secretion can be used as an important component of integrated pest management against Galleria.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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Open peer comments: Debate/Discuss/Question/Opinion

<1>

Dr. Mohite@Agriculture College<pandurang6@yahoo.co.in>

2014-11-07 17:43:40

This will be reference to conduct PG Research to my PG student

REVATHI@STUDENT<revs.agri@gmail.com>

2012-02-07 18:01:41

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