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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.8 P.778~786

http://doi.org/10.1631/jzus.2005.B0778


Active changes of lignification-related enzymes in pepper response to Glomus intraradices and/or Phytophthora capsici


Author(s):  ZHENG Hu-zhe, CUI Chun-lan, ZHANG Yu-ting, WANG Dan, JING Yu, KIM Kil Yong

Affiliation(s):  Division of Biological and Environmental Chemistry, School of Agriculture and Life Science, Chonnam National University, Gwangju 500-757, Korea; more

Corresponding email(s):   zhenghuzhe@yahoo.com.cn

Key Words:  Arbuscular mycorrhizal (AM) fungus, Glomus intraradices, Phytophthora capsici, Peroxidase (POD), Polyphenol oxidase (PPO), Phenylalanine ammonia-lyase (PAL)


ZHENG Hu-zhe, CUI Chun-lan, ZHANG Yu-ting, WANG Dan, JING Yu, KIM Kil Yong. Active changes of lignification-related enzymes in pepper response to Glomus intraradices and/or Phytophthora capsici[J]. Journal of Zhejiang University Science B, 2005, 6(8): 778~786.

@article{title="Active changes of lignification-related enzymes in pepper response to Glomus intraradices and/or Phytophthora capsici",
author="ZHENG Hu-zhe, CUI Chun-lan, ZHANG Yu-ting, WANG Dan, JING Yu, KIM Kil Yong",
journal="Journal of Zhejiang University Science B",
volume="6",
number="8",
pages="778~786",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0778"
}

%0 Journal Article
%T Active changes of lignification-related enzymes in pepper response to Glomus intraradices and/or Phytophthora capsici
%A ZHENG Hu-zhe
%A CUI Chun-lan
%A ZHANG Yu-ting
%A WANG Dan
%A JING Yu
%A KIM Kil Yong
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 8
%P 778~786
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0778

TY - JOUR
T1 - Active changes of lignification-related enzymes in pepper response to Glomus intraradices and/or Phytophthora capsici
A1 - ZHENG Hu-zhe
A1 - CUI Chun-lan
A1 - ZHANG Yu-ting
A1 - WANG Dan
A1 - JING Yu
A1 - KIM Kil Yong
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 8
SP - 778
EP - 786
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0778


Abstract: 
The activities of enzymes responsible for lignification in pepper, pre-inoculation with arbuscular mycorrhizal (AM) fungus of Glomus intraradices and/or infection with pathogenic strain of Phytophthora capsici, and the biological control effect of G. intraradices on Phytophthora blight in pepper were investigated. The experiment was carried out with four treatments: (1) plants pre-inoculated with G. intraradices (Gi), (2) plants pre-inoculated with G. intraradices and then infected with P. capsici (Gi+Pc), (3) plants infected with P. capsici (Pc), and (4) plants without any of the two microorganisms (C). Mycorrhizal colonization rate was reduced by about 10% in pathogen challenged plants. Root mortality caused by infection of P. capsici was completely eliminated by pre-inoculation with antagonistic G. intraradices. On the ninth day after pathogen infection, peroxidase (POD) activity increased by 116.9% in Pc-treated roots but by only 21.2% in Gi+Pc-treated roots, compared with the control, respectively. polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) activities gradually increased during the first 3 d and dramatically decreased in Pc-treated roots but slightly decreased in Gi+Pc-treated roots, respectively. On the ninth day after pathogen infection, PPO and PAL decreased by 62.8% and 73.9% in Pc-treated roots but by only 19.8% and 19.5% in Gi+Pc-treated roots, compared with the control, respectively. Three major POD isozymes (45 000, 53 000 and 114 000) were present in Pc-treated roots, while two major bands (53 000 and 114 000) and one minor band (45 000) were present in spectra of Gi+Pc-treated roots, the 45 000 POD isozyme was significantly suppressed by G. intraradices, suggesting that the 45 000 POD isozyme was induced by the pathogen infection but not induced by the antagonistic G. intraradices. A 60 000 PPO isozyme was induced in Pc-treated roots but not induced in Gi+Pc-treated roots. All these results showed the inoculation of antagonistic G. intraradices alleviates root mortality, activates changes of lignification-related enzymes and induces some of the isozymes in pepper plants infected by P. capsici. The results suggested that G. intraradices is a potentially effective protection agent against P. capsici.

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