Full Text:   <2789>

CLC number: R735

On-line Access: 

Received: 2005-07-28

Revision Accepted: 2005-10-04

Crosschecked: 0000-00-00

Cited: 4

Clicked: 6643

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.12 P.1170-1175

http://doi.org/10.1631/jzus.2005.B1170


Evaluation of ST13 gene expression in colorectal cancer patients


Author(s):  DONG Qing-hua, ZHENG Shu, HU Yue, CHEN Gong-xing, DING Jia-yi

Affiliation(s):  Cancer Institute, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China

Corresponding email(s):   zhengshu@zju.edu.cn

Key Words:  ST13, Colorectal cancer, Real-time PCR


DONG Qing-hua, ZHENG Shu, HU Yue, CHEN Gong-xing, DING Jia-yi. Evaluation of ST13 gene expression in colorectal cancer patients[J]. Journal of Zhejiang University Science B, 2005, 6(12): 1170-1175.

@article{title="Evaluation of ST13 gene expression in colorectal cancer patients",
author="DONG Qing-hua, ZHENG Shu, HU Yue, CHEN Gong-xing, DING Jia-yi",
journal="Journal of Zhejiang University Science B",
volume="6",
number="12",
pages="1170-1175",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B1170"
}

%0 Journal Article
%T Evaluation of ST13 gene expression in colorectal cancer patients
%A DONG Qing-hua
%A ZHENG Shu
%A HU Yue
%A CHEN Gong-xing
%A DING Jia-yi
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 12
%P 1170-1175
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B1170

TY - JOUR
T1 - Evaluation of ST13 gene expression in colorectal cancer patients
A1 - DONG Qing-hua
A1 - ZHENG Shu
A1 - HU Yue
A1 - CHEN Gong-xing
A1 - DING Jia-yi
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 12
SP - 1170
EP - 1175
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B1170


Abstract: 
We identified a novel gene ST13 from a subtractive cDNA library of normal intestinal mucosa in 1993, more studies showed that ST13 was a co-chaperone of Hsp70s. Recently we detected the ST13 gene expression in tumor tissue and adjacent normal tissue of the same colorectal cancer patient and investigated if the ST13 gene expression might have any prognostic value. Analysis was performed at molecular level by reverse transcription-PCR using real-time detection method. We measured two genes simultaneously, ST13 as the target gene and glyceraldehydes-3-phosphate dehydrogenase as a reference gene, in primary colorectal tumor specimens and tumor-adjacent normal mucosa specimens from 50 colorectal cancer patients. The expression levels of the ST13 gene were significantly decreased in primary tumors compared with adjacent mucosa (P<0.05). But there were no significant differences in the expression of ST13 as compared with different Dukes’ stage, tumor differentiation grade, invasion depth, lymph node metastasis and disease-specific survival.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Ballinger, C.A., Connell, P., Wu, Y., Hu, Z., Thompson, L.J., Yin, L.Y., Patterson, C., 1999. Identification of CHIP, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions. Mol. Cell Biol., 19(6):4535-4545.

[2] Bruce, B.D., Churchich, J., 1997. Characterization of the molecular-chaperone function of the heat-shock-cognate-70-interacting protein. Eur. J. Biochem., 245:738-744.

[3] Bukau, B., Horwich, A.L., 1998. The Hsp70 and Hsp60 chaperone machines. Cell, 92:351-366.

[4] Dundas, S.R., Lawrie, L.C., Rooney, P.H., Murray, J.I., 2005. Mortalin is over-expressed by colorectal adenocarcinomas and correlates with poor survival. J. Pathol., 205(1):74-81.

[5] Fan, J.H., Yang, W., Sai, J., Richmond, A., 2002. Hsc/Hsp70 interacting protein (Hip) associates with CXCR2 and regulates the receptor signaling and trafficking. J. Bio. Chem., 277(8):6590-6597.

[6] Gelmini, S., Orlando, C., Sestini, R., Vona, J., Pinzani, P., Giacca, M., Pazzagali, M., 1997. Quantitative polymerase chain reaction-based homogeneous assay with fluorogenic probe to measure c-erB-2 oncogene amplification. Clinical Chemistry, 43(5):752-758.

[7] Hartl, F.U., 1996. Molecular chaperones in cellular protein folding. Nature, 381:571-580.

[8] Hohfeld, J., Jentsch, S., 1997. GrpE-like regulation of the Hsc70 chaperone by the anti-apoptotic protein BAG-1. EMBO J., 16:6209-6216.

[9] Hohfeld, J., Minami, Y., Hartl, F.U., 1995. Hip, a novel cochaperone involved in the eukaryotic Hsc70/Hsp40 reaction cycle. Cell, 83:589-598.

[10] Irmen, H., Hohfeld, J., 1997. Characterization of functional domains of the eukaryotic co-chaperone Hip. J. Bio. Chem., 272(4):2230-2235.

[11] Jemal, A., Tiwari, R.C., Murray, T., Ghafoor, A., Samuels, A., Ward, E., Feuer, E.J., Thun, M.J., 2004. American cancer society. Cancer statistics. CA Cancer J. Clin., 54(1):8-29.

[12] Kikuchi, R., Noguchi, T., Takeno, S., Funada, Y., Moriyama, H., Uchida, Y., 2002. Nuclear BAG-1 expression reflects malignant potential in colorectal carcinomas. Br. J. Cancer, 87(10):1136-1139.

[13] Lazaris, A.C., Theodoropoulos, G.E., Davaris, P.S., Panoussopoulos, D., Nakopoulous, Z., Ittas, C., Golematis, B.C., 1995. Heat shock protein 70 and HLA-DR molecules tissue expression. Prognostic implications in colorectal cancer. Dis. Colon. Rectum., 38(7):739-745.

[14] Mo, Y.Q., Zheng, S., Shen, D.J., 1996. Differential expression of HSU17714 gene in colorectal cancer and normal colonic mucosa. Chinese Journal of Oncology, 18(4):241-243 (in Chinese).

[15] Nollen, E.A., Kabakov, A.E., Brunsting, J.F., Kanon, B., Hohfeld, J., Kampinga, H.H., 2001. Modulation of in vivo Hsp70 chaperone activity by Hip and BAG-1. J. Biol. Chem., 276:4677-4682.

[16] Nylandsted, J., Rohde, M., Brand, K., Bastholm, L., Elling, F., Jaattela, M., 2000. Selective depletion of heat shock protein 70 (Hsp70) activates a tumor-specific death program that is independent of caspase and bypass of Bcl-2. Proc. Natl. Aca. Sci. USA, 97:7871-7876.

[17] Prapapanich, V., Chen, S., Nair, S.C., Rimerman, R.A., Smith, D.F., 1996a. Molecular cloning of human P48, a transient component of progesterone receptor complexes and an Hsp70-binding protein. Molecular Endocrinology, 10(4):420-431.

[18] Prapapanich, V., Chen, S., Toran, E.G., Rimerman, R.A., Smith, D.F., 1996b. Mutational analysis of the hsp70-interacting protein Hip. Molecular and Cellular Biology, 16(1):6200-6207.

[19] Ravagnan, L., Gurbuxani, S., Susin, S.A., Maisse, C., Daugas, E., Zamzami, N., Mak, T., Jaattela, M., Penninger, J.M., Garrido, C., Kroemer, G., 2001. Heat-shock protein 70 antagonizes apoptosis-inducing factor. Nature-Cell Biol., 3:839-843.

[20] Swan, D.C., Tucker, R.A., Holloway, B.P., Icenogle, J.P., 1997. A sensitive, type-specific, fluorogenic probe assay for detection of human papillomavirus DNA. J. Clin. Microbiol., 35(4):886-891.

[21] Vogelstein, B., Fearon, E.R., 1988. Genetic alterations during colorectal tumor development. N. Engl. J. Med., 312(9):525.

[22] Ye, F., Fang, S.C., Zheng, S., 2001. In situ analysis the expression of new ST13 gene in colorectal cancer and its prognostic value. Journal of Practical Oncology, 16(5):318-321 (in Chinese).

[23] Zheng, S., 1993. Application of subtractive hybridization in screening for colorectal cancer-related genes. Chin. Med. J., 8:100.

[24] Zheng, S., 1997. Recent study on colorectal cancer in China: early detection and novel related gene. Chin. Med. J., 110(4):309-310.

[25] Zheng, S., Cai, X.H., Cao, J., Geng, L.Y., 1997. Screening and identification of down-regulated genes in colorectal carcinoma by subtractive hybridization: a method to identify putative tumor suppressor genes. Chin. Med. J., 110(7):543-549.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE