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Received: 2005-07-28

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.12 P.1170-1175


Evaluation of ST13 gene expression in colorectal cancer patients

Author(s):  DONG Qing-hua, ZHENG Shu, HU Yue, CHEN Gong-xing, DING Jia-yi

Affiliation(s):  Cancer Institute, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China

Corresponding email(s):   zhengshu@zju.edu.cn

Key Words:  ST13, Colorectal cancer, Real-time PCR

DONG Qing-hua, ZHENG Shu, HU Yue, CHEN Gong-xing, DING Jia-yi. Evaluation of ST13 gene expression in colorectal cancer patients[J]. Journal of Zhejiang University Science B, 2005, 6(12): 1170-1175.

@article{title="Evaluation of ST13 gene expression in colorectal cancer patients",
author="DONG Qing-hua, ZHENG Shu, HU Yue, CHEN Gong-xing, DING Jia-yi",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Evaluation of ST13 gene expression in colorectal cancer patients
%A DONG Qing-hua
%A HU Yue
%A CHEN Gong-xing
%A DING Jia-yi
%J Journal of Zhejiang University SCIENCE B
%V 6
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%P 1170-1175
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B1170

T1 - Evaluation of ST13 gene expression in colorectal cancer patients
A1 - DONG Qing-hua
A1 - ZHENG Shu
A1 - HU Yue
A1 - CHEN Gong-xing
A1 - DING Jia-yi
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 12
SP - 1170
EP - 1175
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B1170

We identified a novel gene ST13 from a subtractive cDNA library of normal intestinal mucosa in 1993, more studies showed that ST13 was a co-chaperone of Hsp70s. Recently we detected the ST13 gene expression in tumor tissue and adjacent normal tissue of the same colorectal cancer patient and investigated if the ST13 gene expression might have any prognostic value. Analysis was performed at molecular level by reverse transcription-PCR using real-time detection method. We measured two genes simultaneously, ST13 as the target gene and glyceraldehydes-3-phosphate dehydrogenase as a reference gene, in primary colorectal tumor specimens and tumor-adjacent normal mucosa specimens from 50 colorectal cancer patients. The expression levels of the ST13 gene were significantly decreased in primary tumors compared with adjacent mucosa (P<0.05). But there were no significant differences in the expression of ST13 as compared with different Dukes’ stage, tumor differentiation grade, invasion depth, lymph node metastasis and disease-specific survival.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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