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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.6 P.429-435


Guava extract (Psidium guajava) alters the labelling of blood constituents with technetium-99m


Affiliation(s):  Department of Biophysics and Biometry, Institute of Biology Roberto Alcantara Gomes, University of Estate of Rio de Janeiro, Rio de Janeiro 20551030, Brazil; more

Corresponding email(s):   adenilso@uerj.br

Key Words:  Antioxidant, Blood constituents, Psidium guajava, Technetium-99m

ABREU P.R.C., ALMEIDA M.C., BERNARDO R.M., BERNARDO L.C., BRITO L.C., GARCIA E.A.C., FONSECA A.S., BERNARDO-FILHO M.. Guava extract (Psidium guajava) alters the labelling of blood constituents with technetium-99m[J]. Journal of Zhejiang University Science B, 2006, 7(6): 429-435.

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journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Guava extract (Psidium guajava) alters the labelling of blood constituents with technetium-99m
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%P 429-435
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0429

T1 - Guava extract (Psidium guajava) alters the labelling of blood constituents with technetium-99m
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 6
SP - 429
EP - 435
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0429

Psidium guajava (guava) leaf is a phytotherapic used in folk medicine to treat gastrointestinal and respiratory disturbances and is used as anti-inflammatory medicine. In nuclear medicine, blood constituents (BC) are labelled with technetium-99m (99mTc) and used to image procedures. However, data have demonstrated that synthetic or natural drugs could modify the labelling of BC with 99mTc. The aim of this work was to evaluate the effects of aqueous extract of guava leaves on the labelling of BC with 99mTc. Blood samples of Wistar rats were incubated with different concentrations of guava extract and labelled with 99mTc after the percentage of incorporated radioactivity (%ATI) in BC was determined. The results suggest that aqueous guava extract could present antioxidant action and/or alters the membrane structures involved in ion transport into cells, thus decreasing the radiolabelling of BC with 99mTc. The data showed significant (P<0.05) alteration of ATI in BC from blood incubated with guava extract.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Abdelrahim, S.I., Almadboul, A.Z., Omer, M.E.A., Elegami, A., 2002. Antimicrobial activity of Psidium guajava L. Fitoterapia, 73(7-8):713-715.

[2] Aguilar, A., Argueta, A., Cano, L., 1994. Flora Medicinal Indigena de Mexico. Treinta Y Cinco Monografias del Atlas de las Plantas de la Medicina Tradicional Mexicana. INI, Mexico, p.245 (in Spanish).

[3] Arima, H., Danno, G., 2002. Isolation of antimicrobial compounds from guava (Psidium guajava L.) and their structural elucidation. Bioscience Biotechnology and Biochemistry, 66(8):1727-1730.

[4] Artiko, V., Obradovic, V., Petrovic, M., Perisic-Savic, M., Suvajdzic, N., 2004. Hemangioma of the spleen confirmed by blood pool scintigraphy. Clinical Nuclear Medicine, 29:670-671.

[5] Batick, M.J., 1984. Ethnobotany of Palms in the Neotropics. In: Prance, G.T., Kallunki, J.A. (Eds.), Advances in Economic Botany: Ethnobotany in the Neotropics. New York Botanical Garden, New York, USA, p.9-23.

[6] Begum, S., Hassan, S.I., Siddiqui, B.S., Shaheen, F., Ghayur, M.N., Gilani, A.H., 2002. Triterpenoids from the leaves of Psidium guajava. Phytochemistry, 61(4):399-403.

[7] Begum, S., Hassan, S.I., Ali, S.N., Siddiqui, B.S., 2004. Chemical constituents from the leaves of Psidium guajava. Natural Product Research, 18(2):135-140.

[8] Bernardo-Filho, M., Moura, I.N.S., Boasquevisque, E.M., 1983. 99m-technetium-labelled red blood cells “in vitro”. Arquives of Biological Tecnology, 26:455-461.

[9] Bernardo-Filho, M., Caniné, M.S., Lopes, R.L.F., Boasquevisque, E.M., 1986. Effect of temperature on the “in vitro” labelling of red blood cells with technetium-99m. Arquives of Biological Tecnology, 29:407-412.

[10] Callahan, R.J., Rabito, C.A., 1990. Radiolabelling of erythrocytes with technetium-99m: role of band-3 protein in the transport of pertechnetate across the cell membrane. Journal of Nuclear Medicine, 31:2004-2010.

[11] Chulasiri, M., Suthienkul, O., Pavaro, C., Wongkrajang, Y., 1986. Herbal extracts for diarrheal treatment: antibacterial activity in vitro. Journal of Public Health, 16:21-35.

[12] Conde Garcia, E.A., Nascimento, V.T., Santiago Santos, A.B., 2003. Inotropic effects of extracts of Psidium guajava L. (guava) leaves on the guinea pig atrium. Brazilian Journal of Medical and Biological Research, 36(5):661-668.

[13] Cortes, J., Alonso, J.I., Ruiz-Oliva, F., Alvarez, S., Ormijana, J.S., Caton, B., Alcorta, P., 2003. Renal cell carcinoma detected on Tc-99m-labelled red blood cell imaging. Clinical Nuclear Medicine, 28:920-922.

[14] Cuellar, A.C., Lara, R.A., Zayas, J.P., 1984. Psidium guajava L. tamizaje fotiquímico y estudio del aceite essencial. Revista Cubana de Farmacia, 18:92-99 (in Spanish).

[15] de Oliveira, J.F., de Oliveira, M.B.N., Ávila, A.S., Braga, A.C.S., Catanho, M.T.J.A., Jales, R.L.C., Cardoso, V.N., Bernardo-Filho, M., 2003a. Assessment of the effect of Fucus vesiculosus extract on the labelling of blood constituents with technetium-99m and the histological modifications on the shape of the red blood cells. Food and Chemical Toxicology, 41(1):15-20.

[16] de Oliveira, J.F., Santos-Filho, S.D., Catanho, M.T.J.A., Srivastava, S.C., Lima-Filho, G.L., Bernardo-Filho, M., 2003b. Effect of extract of medicinal plants on the labelling of blood elements with technetium-99m and on the morphology of red blood cells (RBC): toxicological actions of roast coffee beans (Coffea arabica). Indian Journal of Nuclear Medicine, 18:52-56.

[17] Dewanjee, M.K., Rao, S.A., Penniston, J.T., 1982. Mechanism of red blood cell labelling with 99mTechnetium-pertecnetate and the role of cation pumps at RBC membrane on distribution and binding of Sn+2 and 99mTechnetium with membrane proteins and hemoglobin. Journal of Labelled Compounds and Radiopharmaceuticals, 11:1464-1466.

[18] Early, P.J., Soddee, D.B., 1995. Principles and Practice of Nuclear Medicine. Mosby—Year Book, Inc., Toronto, Canada, p.877.

[19] Fonseca, A.S., Frydman, J.N.G., Santos, R., Bernardo-Filho, M., 2005. Influence of antipyretic drugs on the labelling of blood elements with technetium-99m. Acta Biologica Hungarica, 56(3-4):275-282.

[20] Frydman, J.N.G., Oliveira, M.B.N., Santos, A.E.O., Fonseca, A.S., Santos, R., Bernardo-Filho, M., 2004. Influence of methylxanthines on the labelling of blood elements with 99mTechnetium. Pakistan Journal of Biological Science, 4:521-524.

[21] Goncalves, J.L.S., Lopes, R.C., Oliveira, D.B., Costa, S.S., Miranda, M.M.F.S., Romano, M.T.V., Santos, N.S.O., Wigg, M.D., 2005. In vitro anti-rotavirus activity of some medicinal plants used in Brazil against diarrhea. Journal of Ethnopharmacology, 99(3):403-407.

[22] Gutfilen, B., Ribeiro, B.L.A.R., Mattos, M.F., Ribeiro, C.R., Bernardo-Filho, M., 1992. Labelling of thymine with technetium-99m: suggestion of a chemical model. Arquives of Biological Tecnology, 39:69-74.

[23] Harel, F., Dupuis, J., Benelfassi, A., Ruel, N., Gregoire, J., 2005. Radionuclide plethysmography for non-invasive evaluation of peripheral arterial blood flow. American Journal of Physiology, Heart and Circulation Physiology, 289(1):H258-H262.

[24] Jaiarj, P., Khoohaswan, P., Wongkrajang, Y., Peungvicha, P., Suriyawong, P., Saraya, M.L.S., Ruangsomboon, O., 1999. Anticough and antimicrobial activities of Psidium guajava Linn. Leaf extract. Journal of Ethnopharmacology, 67(2):203-212.

[25] Jin, R.B., Ma, X.L., Wen, J.L., Tang, W.J., 2004. Application of radionuclide imaging to hepatic impact injury in rabbits. Chinese Journal of Traumatology, 7:45-48.

[26] Khan, M.L.H., Ahmad, J., 1985. A pharmacognostic study of Psidium guajava L. International Journal of Crude Drug Research, 23:95-103.

[27] Kimura, S., Tamaki, T., Aoki, N., 1985. Acceleration of fibrinolysis by the N-terminal peptide of alpha 2-plasmin inhibitor. Blood, 66:157-160.

[28] Liu, H.N., 1988. Chinese Medicinal Herbs of Hong Kong, Vol. 2. Hang Chiewing As Kwang, Hong Kong, p.104-105.

[29] Lozoya, X., 1999. Xiuhpatli, Herba officinalis. SSA/UNAM, Mexico, p.127 (in Spanish).

[30] Lozoya, X., Meckes, M., Aboud-Zaid, M., Tortoriello, J., Nozolillo, C., Arnason, J.T., 1994. Quercetine glycosides in Psidium guajava L. leaves and determination of a spasmolytic principle. Archives of Medical Research, 25:11-15.

[31] Lutterodt, G.D., 1992. Inhibition of Microlax-induced experimental diarrhea with narcotic-like extracts of Psidium guajava leaf in rats. Journal of Ethnopharmacology, 37(2):151-157.

[32] Lutterodt, G.D., Maleque, A., 1988. Effects on mice locomotor activity of a narcotic-like principle from Psidium guajava leaves. Journal of Ethnopharmacology, 24(2-3):219-231.

[33] Meckes, M., Calzada, F., Tortoriello, J., González, J.L., Martinez, M., 1996. Terpenoids isolated from Psidium guajava with depresant activity on central nervous system. Phytotherapy Research, 10(7):600-603.

[34] Mercadante, A.Z., Steck, A., Pfander, H., 1999. Carotenoids from guava (Psidium guajava l.): isolation and structure elucidation. Journal of Agricultural and Food Chemistry, 47(1):145-151.

[35] Morales, M.A., Trotoriello, J., Meckes, M., Paz, D., Lozoya, X., 1994. Calcium-antagonist effect of quercetin and its relation with the spasmolytic properties of Psidium guajava L. Archives of Medical Research, 25:17-21.

[36] Moreno, S.R., Freitas, R.S., Rocha, E.K., Lima-Filho, G.L., Bernardo-Filho, M., 2004. Protection of plasmid DNA by a Ginkgo biloba extract from the effects of stannous chloride and the action on the labelling of blood elements with technetium-99m. Brazilian Journal of Medical and Biological Research, 37(2):267-271.

[37] Olds, G.D., Cooper, G.S., Chak, A., Sivak, M.V.Jr, Chitale, A.A., Wong, R.C., 2005. The yield of bleeding scans in acute lower gastrointestinal hemorrhage. Journal of Clinical Gastroenterology, 39(4):273-277.

[38] Osman, A.M., Younes, M.E., Sheta, A.E., 1974. Triterpenoids of the leaves of Psidium guajava. Phytochemistry, 13(9):2015-2016.

[39] Podhajcer, O.L., Friedlander, M., Graziani, Y., 1980. Effect of liposome-encapsulated quercetin on DNA synthesis, lactate production, and cyclic adenosine 3':5'-monophosphate level in Ehrlich ascites tumor cells. Cancer Research, 40:1344-1350.

[40] Qa’dan, F., Thewaini, A.J., Ali, D.A., Affi, R., Elkhawad, A., Matalka, K.Z., 2005. The antimicrobial activities of Psidium guajava and Juglans regia leaf extracts to acne-developing organisms. The American Journal of Chinese Medicine [An International Journal of Comparative Medicine East and West], 33(2):197-204.

[41] Qian, H., Nihorimbere, V., 2004. Antioxidant power of phytochemicals from Psidium guajava leaf. Journal of Zhejiang University Science, 5(6):676-683.

[42] Re, L., Barocci, S., Capitani, C., Vivani, C., Ricci, M., Rinaldi, L., Paolucci, G., Scarpantonio, A., Leon-Fernandez, O.S., Morales, M.A., 1999. Effects of some natural extracts on the acetylcholine release at the mouse neuromuscular junction. Pharmacological Research, 39(3):239-245.

[43] Saha, G.B., 2003. Normal Biodistribution of Diagnostic Radiopharmaceuticals. In: Hladik, W.B., Saha, G.B., Study, K.T. (Eds.), Essentials of Nuclear Medicine Science. Williams and Wilkins, Baltimore, USA, p.3-19.

[44] Sampson, C.B., 1996. Complications and difficulties in radiolabelling blood cells: a review. Nuclear Medicine Communications, 17:648-658.

[45] Slart, R.H., Phan, T.T., Talsma, M.D., Jager, P.L., 2004. Different splenic uptake of Tc-99m sulfur colloid and Tc-99m heat-denatured red blood cells in an infant with complete situs inversus. Clinical Nuclear Medicine, 29:590-591.

[46] Valenca, S.S., Lima, E.A., Diré, G.F., Bernardo-Filho, M., Porto, L.C., 2005. Sodium pertechnetate (Na99mTcO4) biodistribution in mice exposed to cigarette smoke. BMC Nuclear Medicine, 5(1):1.

[47] Vanacker, S.A.B.E., Tromp, M.N.J.L., Haenen, G.R.M.M., Vandervijgh, W.J.F., Bast, A., 1995. Flavonoids as scavengers of nitric oxide radical. Biochemical and Biophysical Research Communications, 214(3):755-759.

[48] Verdu, J., Martinez, A., Anton, M.A., Munoz, J.M., Riera, M., Jover, R., Caballero, O., 2005. Increased thallium-201 uptake and Tc-99m red blood cell accumulation in hemangioma. Clinical Nuclear Medicine, 30(1):25-26.

[49] Wong, K.T., Beauvais, M.M., Melchior, W.R., Snyder, S.P., 2004. Enhanced liver uptake of Tc-99m-labelled RBCs during gastrointestinal bleed scintigraphy using transfused RBCs compared with autologous RBCs. Clinical Nuclear Medicine, 29:522-523.

[50] Zaman, M.U., Hussain, R., Sajjad, Z., Ahmad, M.N., 2004. Localization of upper GI bleed on delayed imaging with 99mTc tagged RBC angiogram. Journal of Pakistan Medicine Association, 54:490-491.

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