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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.8 P.608~614

http://doi.org/10.1631/jzus.2006.B0608


Antitumor activities of D-glucosamine and its derivatives


Author(s):  ZHANG Li, LIU Wan-shun, HAN Bao-qin, PENG Yan-fei, WANG Dong-feng

Affiliation(s):  College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; more

Corresponding email(s):   qdzhangli@ouc.edu.cn, wangdf@ouc.edu.cn

Key Words:  D-glucosamine hydrochloride, D-glucosamine, N-acetyl glucosamine, Antitumor, Human hepatoma cell, Sarcoma 180


ZHANG Li, LIU Wan-shun, HAN Bao-qin, PENG Yan-fei, WANG Dong-feng. Antitumor activities of D-glucosamine and its derivatives[J]. Journal of Zhejiang University Science B, 2006, 7(8): 608~614.

@article{title="Antitumor activities of D-glucosamine and its derivatives",
author="ZHANG Li, LIU Wan-shun, HAN Bao-qin, PENG Yan-fei, WANG Dong-feng",
journal="Journal of Zhejiang University Science B",
volume="7",
number="8",
pages="608~614",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0608"
}

%0 Journal Article
%T Antitumor activities of D-glucosamine and its derivatives
%A ZHANG Li
%A LIU Wan-shun
%A HAN Bao-qin
%A PENG Yan-fei
%A WANG Dong-feng
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 8
%P 608~614
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0608

TY - JOUR
T1 - Antitumor activities of D-glucosamine and its derivatives
A1 - ZHANG Li
A1 - LIU Wan-shun
A1 - HAN Bao-qin
A1 - PENG Yan-fei
A1 - WANG Dong-feng
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 8
SP - 608
EP - 614
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0608


Abstract: 
The growth inhibitory effects of d-glucosamine hydrochloride%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>d-glucosamine hydrochloride (GlcNH2∙HCl), d-glucosamine (GlcNH2) and n-acetyl glucosamine (NAG) on human hepatoma SMMC-7721 cells in vitro were investigated. The results showed that GlcNH2∙HCl and GlcNH2 resulted in a concentration-dependent reduction in hepatoma cell growth as measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. This effect was accompanied by a marked increase in the proportion of S cells as analyzed by flow cytometry. In addition, human hepatoma SMMC-7721 cells treated with GlcNH2∙HCl resulted in the induction of apoptosis as assayed qualitatively by agarose gel electrophoresis. NAG could not inhibit the proliferation of SMMC-7721 cells. GlcNH2∙HCl exhibited antitumor activity against sarcoma 180 in Kunming mice at dosage of 125~500 mg/kg, dose of 250 mg/kg being the best. GlcNH2∙HCl at dose of 250 mg/kg could enhance significantly the thymus index, and spleen index and could promote T lymphocyte proliferation induced by ConA. The antitumor effect of GlcNH2∙HCl is probably host-mediated and cytocidal.

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