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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.8 P.608~614


Antitumor activities of D-glucosamine and its derivatives

Author(s):  ZHANG Li, LIU Wan-shun, HAN Bao-qin, PENG Yan-fei, WANG Dong-feng

Affiliation(s):  College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; more

Corresponding email(s):   qdzhangli@ouc.edu.cn, wangdf@ouc.edu.cn

Key Words:  D-glucosamine hydrochloride, D-glucosamine, N-acetyl glucosamine, Antitumor, Human hepatoma cell, Sarcoma 180

ZHANG Li, LIU Wan-shun, HAN Bao-qin, PENG Yan-fei, WANG Dong-feng. Antitumor activities of D-glucosamine and its derivatives[J]. Journal of Zhejiang University Science B, 2006, 7(8): 608~614.

@article{title="Antitumor activities of D-glucosamine and its derivatives",
author="ZHANG Li, LIU Wan-shun, HAN Bao-qin, PENG Yan-fei, WANG Dong-feng",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Antitumor activities of D-glucosamine and its derivatives
%A LIU Wan-shun
%A HAN Bao-qin
%A PENG Yan-fei
%A WANG Dong-feng
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 8
%P 608~614
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0608

T1 - Antitumor activities of D-glucosamine and its derivatives
A1 - LIU Wan-shun
A1 - HAN Bao-qin
A1 - PENG Yan-fei
A1 - WANG Dong-feng
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 8
SP - 608
EP - 614
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0608

The growth inhibitory effects of d-glucosamine hydrochloride%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>d-glucosamine hydrochloride (GlcNH2∙HCl), d-glucosamine (GlcNH2) and n-acetyl glucosamine (NAG) on human hepatoma SMMC-7721 cells in vitro were investigated. The results showed that GlcNH2∙HCl and GlcNH2 resulted in a concentration-dependent reduction in hepatoma cell growth as measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. This effect was accompanied by a marked increase in the proportion of S cells as analyzed by flow cytometry. In addition, human hepatoma SMMC-7721 cells treated with GlcNH2∙HCl resulted in the induction of apoptosis as assayed qualitatively by agarose gel electrophoresis. NAG could not inhibit the proliferation of SMMC-7721 cells. GlcNH2∙HCl exhibited antitumor activity against sarcoma 180 in Kunming mice at dosage of 125~500 mg/kg, dose of 250 mg/kg being the best. GlcNH2∙HCl at dose of 250 mg/kg could enhance significantly the thymus index, and spleen index and could promote T lymphocyte proliferation induced by ConA. The antitumor effect of GlcNH2∙HCl is probably host-mediated and cytocidal.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Akiyama, K., Kawazu, K., Kobayashi, A., 1995. A novel method for chemo-enzymatic synthesis of elicitor-active chitosan oligomers and partially N-deacetylated chitin oligomers using N-acylated chitotrioses as substrates in a lysozyme-catalyzed transglycosylation reaction system. Carbohydrate Research, 279:151-160.

[2] Borner, M.M., Jonocourt, F., Hotz, M.A., 1997. Similarity of apoptosis induction by 2-chlorodeoxyadenosine and cisplatin in human mononuclear blood cells. British Journal of Cancer, 76(11):1448-1454.

[3] Bortner, C.D., Cidlowski, N.B.E., Oldenburg, J.A., 1995. The role of DNA fragmentation in apoptosis. Trends in Cell Biology, 5(1):21-26.

[4] Chandy, T., Sharma, C.P., 1990. Chitosan―as a biomaterial. Journal of Biomaterials, Artificial Cells & Artificial Organs, 18(1):1-24.

[5] Chen, X., 2001. Study of antimicrobial effects of D-glucosamine hydrochloride. Fine Chemicals, 18(2):78-79 (in Chinese).

[6] Chen, Q., Yang, F., Du, Y.G., 2005. Synthesis of a C-3-symmetric (1→6)-N-acetyl-beta-D-glucosamine octadecasaccharide using click chemistry. Carbohydrate Research, 340(16):2476-2482.

[7] Dong, B.X., Wang, Z., Liang, R., Chen, X.Q., Huang, G.S., Qiao, Y., Wang, A.Q., 2004. Inhibition of proliferation and induction of differentiation of leukemia cell line HL60 by glucosamine sulphate. Journal of the Fourth Military Medical University, 25(5):424-427 (in Chinese).

[8] Friedman, S.J., Skehan, P., 1980. Membrane-active drugs potentiate the killing of tumor cell by D-glucosamine. Proceedings of the National Academy of Sciences of the United States of America, 77(2):1172-1176.

[9] Hengartner, M.G., 2000. The biochemistry of apoptosis. Nature, 407(6805):770-775.

[10] Ingle, T.R., Vaidya, S.H., Pai, M.U., 1973. Production of D-glucosamine hydrochlo ride (GAH) from fish canning waste. Research and Industry, 18(2):54-56.

[11] Kearse, K.P., Hart, G.W., 1991. Lymphocyte activation induces rapid changes in nuclear and cytoplasmic glycoproteins. Proceedings of the National Academy of Sciences of the United States of America, 88(5):1701-1705.

[12] Kivinen, K., Kallajoki, M., Taimen, P., 2005. Caspase-3 is required in the apoptotic disintegration of the nuclear matrix. Experimental Cell Research, 311(1):62-73.

[13] Kolm-Litty, V., Sauer, U., Nerlich, A., Lehmann, R., Schleicher, E.D., 1998. High glucose-induced transforming growth factor β1 production is mediated by the hexosamine pathway in porcine glomerular mesangial cells. Journal of Clinical Investigation, 101(1):160-169.

[14] Kosmider, B., Osiecka, R., Ciesielska, E., Szmigiero, L., Zyner, E., Ochocki, J., 2004. Induction of apoptosis and necrosis in lymphocytes by the cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 558(1-2):169-179.

[15] Lin, P.S., Ho, K.C., Yang, S.J., 1996. Tirapazamine (SR 4233) interrupts cell cycle progression and induces apoptosis. Cancer Letters, 105(2):249-255.

[16] Martin, G.G., Castro, C., Moy, N., Rubin, N., 2003. N-acetyl-D-glucosamine in crustacean hemocytes; possible functions and usefulness in hemocyte classification. Invertebrate Biology, 122(3):265-270.

[17] Mosmann, T., 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2):55-56.

[18] Muzzarelli, R.A.A., 1993. In vivo Biochemical Significance of Chitin-Based Medical Items. In: Dumitriu, S. (Ed.), Polymeric Biomaterials. Marcel Dekker, New York, p.179-197.

[19] Nakashima, T., Uemura, T., Maehara, Y., Sugimachi, K., 1989. Succinate dehydrogenase inhibition test for evaluating head and neck tumors. Oncology, 46(3):162-168.

[20] Olsen, R., Schwartzmiller, D., Weppner, W., Winardy, R., 1989. Biomedical Applications of Chitin and Its Derivatives. In: Skjak-Braek, G., Anthonsen, T., Sandford, P. (Eds.), Chitin and Chitosan. Elsevier, London, p.813-828.

[21] Ormerod, M.G., O’Neill, C.F., Robertson, D., Harrap, K.R., 1994. Cisplatin induces apoptosis in a human ovarian carcinoma cell line without concomitant internucleosomal degradation of DNA. Experimental Cell Research, 211(2):231-237.

[22] Ouyang, P.K., Zhang, G.D., Qi, J.Y., Zhang, L.Y., 2000. Handbook of Chemical Products, 3rd Ed. Chemical Industry Press, Beijing, p.368 (in Chinese).

[23] Reason, A.J., Morris, H.R., Panico, M., Marais, R., Treisman, R.H., Haltiwanger, R.S., Hart, G.W., Kelly, W.G., Dell, A., 1992. Localization of O-GlcNAc modification on the serum response transcription factor (SRF). Journal of Biological Chemistry, 267(24):16911-16921.

[24] Reginster, J.Y., Deroisy, R., Rovati, L.C., Lee, R.L., Lejeune, E., Bruyere, O., Giacovelli, G., Henrotin, Y., Dacre, J.E., Gossett, C., 2001. Long-term effects of glucosamine sulphate on osteoarthritis progression: a radomised, placebo-controlled clinical trial. The Lancet, 357(9252):251-256.

[25] Sakahira, H., Enari, M., Ohsawa, Y., Uchiyama, Y., Nagata, S., 1999. Apoptotic nuclear morphological change without DNA fragmentation. Current Biology, 9(10):543-546.

[26] Sambrook, J., Russell, D., 2001. Molecular Cloning: A Laboratry Manual. Cold Spring Harbor Lab(CSHL) Press, New York, p.463-468.

[27] Santini, M.T., Cametti, C., Indovina, P.L., Morelli, G., Donelli, G., 1997. Polylysine induces changes in membrane electrical properties of K562 cells. Journal of Biomedical Materials Research, 35(2):165-174.

[28] Schipper, N.G., Varum, K.M., Artursson, P., 1996. Chitosans as absorption enhancers for poorly absorbable drugs. 1: Influence of molecular weight and degree of acetylation on drug transport across human intestinal epithelial (Caco-2) cells. Pharmaceutical Research, 13(11):1686-1692.

[29] Schulze-Osthoff, K., Walczak, H., Dröge, W., Krammer, P.H., 1994. Cell nucleus and DNA fragmentation are not required for apoptosis. Journal of Cell Biology, 127(1):15-20.

[30] Tsukada, K., Matsumoto, T., Aizawa, K., Tokoro, A., Naruse, R., Suzuki, S., Suzuki, M., 1990. Antimetastatic and growth-inhibitory effects of N-acetylchitohexaose in mice bearing Lewis lung carcinoma. Japanese Journal of Cancer Research, 81(3):259-265.

[31] Wang, S.H., Chen, J.C., 2005. The protective effect of chitin and chitosan against Vibrio alginolyticus in white shrimp Litopenaeus vannamei. Fish & Shellfish Immunology, 19(3):191-204.

[32] Wang, Z., Qiao, Y., Huang, G.S., Wang, A.Q., Zhang, Y.Q., Feng, J.L., Yang, G.R., Guo, Y., Liang, R., 2003a. Glucosamine and glucosamine hydrochloride induced leukemia cell line K562 differentiation into macrophage. Chinese Pharmacological Bulletin, 19(3):290-293 (in Chinese).

[33] Wang, Z., Qiao, Y., Huang, G.S., Wang, A.Q., Zhang, Y.Q., Feng, J.L., Yang, G.R., Guo, Y., Liang, R., 2003b. Induction of macrophagic differentiation of leukemia cell line K562 by N-acetyl-D-glucosamine. Journal of the Fourth Military Medical University, 24(1):46-48 (in Chinese).

[34] Wilson, J.K., Sargent, J.M., Elgie, A.W., Hill, J.G., Taylor, C.G., 1990. A feasibility study of the MTT assay for chemosensitivity testing in ovarian malignancy. British Journal of Cancer, 62(2):189-194.

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