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Abstract: The growth inhibitory effects of d-glucosamine hydrochloride%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>d-glucosamine hydrochloride (GlcNH₂∙HCl), d-glucosamine (GlcNH₂) and n-acetyl glucosamine (NAG) on human hepatoma SMMC-7721 cells in vitro were investigated. The results showed that GlcNH₂∙HCl and GlcNH₂ resulted in a concentration-dependent reduction in hepatoma cell growth as measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. This effect was accompanied by a marked increase in the proportion of S cells as analyzed by flow cytometry. In addition, human hepatoma SMMC-7721 cells treated with GlcNH₂∙HCl resulted in the induction of apoptosis as assayed qualitatively by agarose gel electrophoresis. NAG could not inhibit the proliferation of SMMC-7721 cells. GlcNH₂∙HCl exhibited antitumor activity against sarcoma 180 in Kunming mice at dosage of 125~500 mg/kg, dose of 250 mg/kg being the best. GlcNH₂∙HCl at dose of 250 mg/kg could enhance significantly the thymus index, and spleen index and could promote T lymphocyte proliferation induced by ConA. The antitumor effect of GlcNH₂∙HCl is probably host-mediated and cytocidal.

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