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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.10 P.825~829

http://doi.org/10.1631/jzus.2006.B0825


Inhibitive effects of anti-oxidative vitamins on mannitol-induced apoptosis of vascular endothelial cells


Author(s):  PAN Kai-yu, SHEN Mei-ping, YE Zhi-hong, DAI Xiao-na, SHANG Shi-qiang

Affiliation(s):  Affiliated Children’ more

Corresponding email(s):   beyondmy2002@163.com, shangsq33@mail.hz.zj.cn

Key Words:  Anti-oxidative vitamins, Inhibitive effects, Apoptosis, Vascular endothelial cells, Mannitol


PAN Kai-yu, SHEN Mei-ping, YE Zhi-hong, DAI Xiao-na, SHANG Shi-qiang. Inhibitive effects of anti-oxidative vitamins on mannitol-induced apoptosis of vascular endothelial cells[J]. Journal of Zhejiang University Science B, 2006, 7(10): 825~829.

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author="PAN Kai-yu, SHEN Mei-ping, YE Zhi-hong, DAI Xiao-na, SHANG Shi-qiang",
journal="Journal of Zhejiang University Science B",
volume="7",
number="10",
pages="825~829",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0825"
}

%0 Journal Article
%T Inhibitive effects of anti-oxidative vitamins on mannitol-induced apoptosis of vascular endothelial cells
%A PAN Kai-yu
%A SHEN Mei-ping
%A YE Zhi-hong
%A DAI Xiao-na
%A SHANG Shi-qiang
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 10
%P 825~829
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0825

TY - JOUR
T1 - Inhibitive effects of anti-oxidative vitamins on mannitol-induced apoptosis of vascular endothelial cells
A1 - PAN Kai-yu
A1 - SHEN Mei-ping
A1 - YE Zhi-hong
A1 - DAI Xiao-na
A1 - SHANG Shi-qiang
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 10
SP - 825
EP - 829
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0825


Abstract: 
Objective: Study blood vessel injury and gene expression indicating vascular endothelial cell apoptosis induced by mannitol with and without administration of anti-oxidative vitamins. Methods: Healthy rabbits were randomly divided into four groups. mannitol was injected into the vein of the rabbit ear in each animal. Pre-treatment prior to mannitol injection was performed with normal saline (group B), vitamin C (group C) and vitamin E (group D). Blood vessel injury was assessed under electron and light microscopy. In a second experiment, cell culture specimen of human umbilical vein endothelial cells were treated with mannitol. Pre-treatment was done with normal saline (sample B), vitamin C (sample C) and vitamin E (sample D). Total RNA was extracted with the original single step procedure, followed by hybridisation and analysis of gene expression. Results: In the animal experiment, serious blood vessel injury was seen in group A and group B. Group D showed light injury only, and normal tissue without pathological changes was seen in group C. Of all 330 apoptosis-related genes analysed in human cell culture specimen, no significant difference was seen after pre-treatment with normal saline, compared with the gene chip without pre-treatment. On the gene chip pre-treated with vitamin C, 45 apoptosis genes were down-regulated and 34 anti-apoptosis genes were up-regulated. Pre-treatment with vitamin E resulted in the down-regulation of 3 apoptosis genes. Conclusion: Vitamin C can protect vascular endothelial cells from mannitol-induced injury.

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Reference

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