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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.10 P.817~824


Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum

Author(s):  ZHENG Yi-xiong, RINGE Jochen, LIANG Zhong, LOCH Alexander, CHEN Li, SITTINGER Michael

Affiliation(s):  Department of Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   zyx_xxn@hotmail.com

Key Words:  Tissue engineering, Poly-lactic-co-glycolic acid polymer, Periosteum-derived progenitor cells, 3-dimensional culture

ZHENG Yi-xiong, RINGE Jochen, LIANG Zhong, LOCH Alexander, CHEN Li, SITTINGER Michael. Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum[J]. Journal of Zhejiang University Science B, 2006, 7(10): 817~824.

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journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum
%A ZHENG Yi-xiong
%A RINGE Jochen
%A LIANG Zhong
%A LOCH Alexander
%J Journal of Zhejiang University SCIENCE B
%V 7
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%P 817~824
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0817

T1 - Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum
A1 - ZHENG Yi-xiong
A1 - RINGE Jochen
A1 - LIANG Zhong
A1 - LOCH Alexander
A1 - CHEN Li
A1 - SITTINGER Michael
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 10
SP - 817
EP - 824
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2006.B0817

The use of periosteum-derived progenitor cells (PCs) combined with bioresorbable materials is an attractive approach for tissue engineering. The aim of this study was to characterize the osteogenic differentiation of PC in 3-dimensional (3D) poly-lactic-co-glycolic acid (PLGA) fleeces cultured in medium containing allogeneic human serum. PCs were isolated and expanded in monolayer culture. Expanded cells of passage 3 were seeded into PLGA constructs and cultured in osteogenic medium for a maximum period of 28 d. Morphological, histological and cell viability analyses of three-dimensionally cultured PCs were performed to elucidate osseous synthesis and deposition of a calcified matrix. Furthermore, the mRNA expression of type I collagen, osteocalcin and osteonectin was semi-quantitively evaluated by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The fibrin gel immobilization technique provided homogeneous PCs distribution in 3D PLGA constructs. Live-dead staining indicated a high viability rate of PCs inside the PLGA scaffolds. Secreted nodules of neo-bone tissue formation and the presence of matrix mineralization were confirmed by positive von Kossa staining. The osteogenic differentiation of PCs was further demonstrated by the detection of type I collagen, osteocalcin and osteonectin gene expression. The results of this study support the concept that this tissue engineering method presents a promising method for creation of new bone in vivo.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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