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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.1 P.27~32

10.1631/jzus.2007.B0027


Optimization of fermentation conditions for P450 BM-3 monooxygenase production by hybrid design methodology


Author(s):  LU Yan, MEI Le-he

Affiliation(s):  Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   meilh@zju.edu.cn

Key Words:  Optimization, P450 BM-3, Plackett-Burman (PB) design, Hybrid design


LU Yan, MEI Le-he. Optimization of fermentation conditions for P450 BM-3 monooxygenase production by hybrid design methodology[J]. Journal of Zhejiang University Science B, 2007, 8(1): 27~32.

@article{title="Optimization of fermentation conditions for P450 BM-3 monooxygenase production by hybrid design methodology",
author="LU Yan, MEI Le-he",
journal="Journal of Zhejiang University Science B",
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pages="27~32",
year="2007",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.B0027"
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%0 Journal Article
%T Optimization of fermentation conditions for P450 BM-3 monooxygenase production by hybrid design methodology
%A LU Yan
%A MEI Le-he
%J Journal of Zhejiang University SCIENCE B
%V 8
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%P 27~32
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0027

TY - JOUR
T1 - Optimization of fermentation conditions for P450 BM-3 monooxygenase production by hybrid design methodology
A1 - LU Yan
A1 - MEI Le-he
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 1
SP - 27
EP - 32
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2007.B0027


Abstract: 
Factorial design and response surface techniques were used to design and optimize increasing p450 BM-3 expression in E. coli. Operational conditions for maximum production were determined with twelve parameters under consideration: the concentration of FeCl3, induction at OD578 (optical density measured at 578 nm), induction time and inoculum concentration. Initially, plackett-Burman (PB) design was used to evaluate the process variables relevant in relation to p450 BM-3 production. Four statistically significant parameters for response were selected and utilized in order to optimize the process. With the 416C model of hybrid design, response surfaces were generated, and p450 BM-3 production was improved to 57.90×10−3 U/ml by the best combinations of the physicochemical parameters at optimum levels of 0.12 mg/L FeCl3, inoculum concentration of 2.10%, induction at OD578 equal to 1.07, and with 6.05 h of induction.

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Reference

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