Full Text:   <323>

Summary:  <134>

CLC number: 

On-line Access: 2023-11-13

Received: 2023-05-18

Revision Accepted: 2023-07-16

Crosschecked: 2023-11-14

Cited: 0

Clicked: 510

Citations:  Bibtex RefMan EndNote GB/T7714


Ning HU


-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE A 2023 Vol.24 No.11 P.1017-1026


Advances in micro-nano biosensing platforms for intracellular electrophysiology

Author(s):  Jiajin XUE, Min SHAO, Zhigang GAO, Ning HU

Affiliation(s):  General Surgery Department, Childrens Hospital, Zhejiang University School of Medicine, Hangzhou 310052, China; more

Corresponding email(s):   ebwk@zju.edu.cn, huning@zju.edu.cn

Key Words:  Intracellular electrophysiology, Micro-nano biosensing platforms, Cardiology and neuroscience, Intracellular action potentials

Jiajin XUE, Min SHAO, Zhigang GAO, Ning HU. Advances in micro-nano biosensing platforms for intracellular electrophysiology[J]. Journal of Zhejiang University Science A, 2023, 24(11): 1017-1026.

@article{title="Advances in micro-nano biosensing platforms for intracellular electrophysiology",
author="Jiajin XUE, Min SHAO, Zhigang GAO, Ning HU",
journal="Journal of Zhejiang University Science A",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Advances in micro-nano biosensing platforms for intracellular electrophysiology
%A Jiajin XUE
%A Zhigang GAO
%A Ning HU
%J Journal of Zhejiang University SCIENCE A
%V 24
%N 11
%P 1017-1026
%@ 1673-565X
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.A2300267

T1 - Advances in micro-nano biosensing platforms for intracellular electrophysiology
A1 - Jiajin XUE
A1 - Min SHAO
A1 - Zhigang GAO
A1 - Ning HU
J0 - Journal of Zhejiang University Science A
VL - 24
IS - 11
SP - 1017
EP - 1026
%@ 1673-565X
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.A2300267

The establishment of a dependable electrophysiological detection platform is paramount for cardiology and neuroscience research. In the past decade, devices based on micro and nanoscale sensing and control technologies have been developed to construct electrophysiological platforms. Their unique morphological advantages and novel processing methods offer the potential for high-throughput, high-fidelity electrical signal recording. In this review, we analyze the structure, transmembrane strategies, and electrophysiological detection methods of active/passive micro and nano sensing platforms. We also provide an outlook on their vast potential for development in light of the opportunities and challenges facing micro and nano sensing technology, with the aim of pushing for higher-level electrophysiological platform construction to meet the needs of experimental research and clinical applications.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]AbbottJ, YeTY, QinL, et al., 2017. CMOS nanoelectrode array for all-electrical intracellular electrophysiological imaging. Nature Nanotechnology, 12(5):460-466.

[2]AbbottJ, YeTY, HamD, et al., 2018. Optimizing nanoelectrode arrays for scalable intracellular electrophysiology. Accounts of Chemical Research, 51(3):600-608.

[3]AbbottJ, YeTY, KrenekK, et al., 2020. A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons. Nature Biomedical Engineering, 4(2):232-241.

[4]ChenWW, GaoRL, LiuLS, et al., 2017. China cardiovascular diseases report 2015: a summary. Journal of Geriatric Cardiology, 14(1):1-10.

[5]ConnollyP, ClarkP, CurtisASG, et al., 1990. An extracellular microelectrode array for monitoring electrogenic cells in culture. Biosensors and Bioelectronics, 5(3):223-234.

[6]DavieJT, KoleMHP, LetzkusJJ, et al., 2006. Dendritic patch-clamp recording. Nature Protocols, 1(3):1235-1247.

[7]DesbiollesBXE, de CoulonE, BertschA, et al., 2019. Intracellular recording of cardiomyocyte action potentials with nanopatterned volcano-shaped microelectrode arrays. Nano Letters, 19(9):6173-6181.

[8]DipaloM, AminH, LovatoL, et al., 2017. Intracellular and extracellular recording of spontaneous action potentials in mammalian neurons and cardiac cells with 3D plasmonic nanoelectrodes. Nano Letters, 17(6):3932-3939.

[9]DipaloM, MelleG, LovatoL, et al., 2018. Plasmonic meta-electrodes allow intracellular recordings at network level on high-density CMOS-multi-electrode arrays. Nature Nanotechnology, 13(10):965-971.

[10]DipaloM, RastogiSK, MatinoL, et al., 2021. Intracellular action potential recordings from cardiomyocytes by ultrafast pulsed laser irradiation of fuzzy graphene microelectrodes. Science Advances, 7(15):eabd5175.

[11]DuanXJ, GaoRX, XieP, et al., 2012. Intracellular recordings of action potentials by an extracellular nanoscale field-effect transistor. Nature Nanotechnology, 7(3):‍174-179.

[12]FangJR, XuDX, WangH, et al., 2022. Scalable and robust hollow nanopillar electrode for enhanced intracellular action potential recording. Nano Letters, 23(1):243-251.

[13]FromherzP, OffenhäusserA, VetterT, et al., 1991. A neuron-silicon junction: a Retzius cell of the leech on an insulated-gate field-effect transistor. Science, 252(5010):1290-1293.

[14]HaiA, ShappirJ, SpiraME, 2010. In-cell recordings by extracellular microelectrodes. Nature Methods, 7(3):200-202.

[15]HannunAY, RajpurkarP, HaghpanahiM, et al., 2019. Cardiologist-level arrhythmia detection and classification in ambulatory electrocardiograms using a deep neural network. Nature Medicine, 25(1):65-69.

[16]HuN, XuDX, FangJR, et al., 2020. Intracellular recording of cardiomyocyte action potentials by nanobranched microelectrode array. Biosensors and Bioelectronics, 169:112588.

[17]JahedZ, YangY, TsaiCT, et al., 2022. Nanocrown electrodes for parallel and robust intracellular recording of cardiomyocytes. Nature Communications, 13(1):2253.

[18]JohnstoneAFM, GrossGW, WeissDG, et al., 2010. Microelectrode arrays: a physiologically based neurotoxicity testing platform for the 21st century. Neurotoxicology, 31(4):331-350.

[19]LinZC, XieC, OsakadaY, et al., 2014. Iridium oxide nanotube electrodes for sensitive and prolonged intracellular measurement of action potentials. Nature Communications, 5:3206.

[20]LiuR, ChenRJ, ElthakebAT, et al., 2017. High density individually addressable nanowire arrays record intracellular activity from primary rodent and human stem cell derived neurons. Nano Letters, 17(5):2757-2764.

[21]LiuR, LeeJ, TchoeY, et al., 2022. Ultra-sharp nanowire arrays natively permeate, record, and stimulate intracellular activity in neuronal and cardiac networks. Advanced Functional Materials, 32(8):2108378.

[22]Lopez-IzquierdoA, WarrenM, RiedelM, et al., 2014. A near-infrared fluorescent voltage-sensitive dye allows for moderate-throughput electrophysiological analyses of human induced pluripotent stem cell-derived cardiomyocytes. American Journal of Physiology-Heart and Circulatory Physiology, 307(9):H1370-H1377.

[23]MaLY, ChenWW, GaoRL, et al., 2020. China cardiovascular diseases report 2018: an updated summary. Journal of Geriatric Cardiology, 17(1):1.

[24]MatiukasA, MitreaBG, QinMC, et al., 2007. Near-infrared voltage-sensitive fluorescent dyes optimized for optical mapping in blood-perfused myocardium. Heart Rhythm, 4(11):1441-1451.

[25]PantojaR, NagarahJM, StaraceDM, et al., 2004. Silicon chip-based patch-clamp electrodes integrated with PDMS microfluidics. Biosensors and Bioelectronics, 20(3):509-517.

[26]QingQ, JiangZ, XuL, et al., 2014. Free-standing kinked nanowire transistor probes for targeted intracellular recording in three dimensions. Nature Nanotechnology, 9(2):142-147.

[27]RobinsonJT, JorgolliM, ShalekAK, et al., 2012. Vertical nanowire electrode arrays as a scalable platform for intracellular interfacing to neuronal circuits. Nature Nanotechnology, 7(3):180-184.

[28]SpiraME, HaiA, 2013. Multi-electrode array technologies for neuroscience and cardiology. Nature Nanotechnology, 8(2):83-94.

[29]StauferO, WeberS, BengtsonCP, et al., 2019. Adhesion stabilized en Masse intracellular electrical recordings from multicellular assemblies. Nano Letters, 19(5):3244-3255.

[30]ThomasC, SpringerP, LoebG, et al., 1972. A miniature microelectrode array to monitor the bioelectric activity of cultured cells. Experimental Cell Research, 74(1):61-66.

[31]TianBZ, Cohen-KarniT, QingQ, et al., 2010. Three-dimensional, flexible nanoscale field-effect transistors as localized bioprobes. Science, 329(5993):830-834.

[32]TimmisA, TownsendN, GaleC, et al., 2018. European society of cardiology: cardiovascular disease statistics 2017. European Heart Journal, 39(7):508-579.

[33]ValappilRA, BlackJE, BroderickMJ, et al., 2010. Exploring the electrocardiogram as a potential tool to screen for premotor Parkinson’s disease. Movement Disorders, 25(14):2296-2303.

[34]VardiR, GoldentalA, SardiS, et al., 2016. Simultaneous multi-patch-clamp and extracellular-array recordings: single neuron reflects network activity. Scientific Reports, 6:36228.

[35]VoelkerM, FromherzP, 2005. Signal transmission from individual mammalian nerve cell to field-effect transistor. Small, 1(2):206-210.

[36]WangGF, WyskielDR, YangWG, et al., 2015. An optogenetics- and imaging-assisted simultaneous multiple patch-clamp recording system for decoding complex neural circuits. Nature Protocols, 10(3):397-412.

[37]XieC, LinZL, HansonL, et al., 2012. Intracellular recording of action potentials by nanopillar electroporation. Nature Nanotechnology, 7(3):185-190.

[38]XuDX, FangJR, ZhangMY, et al., 2022a. Porous polyethylene terephthalate nanotemplate electrodes for sensitive intracellular recording of action potentials. Nano Letters, 22(6):2479-2489.

[39]XuDX, FangJR, WangH, et al., 2022b. Scalable nanotrap matrix enhanced electroporation for intracellular recording of action potential. Nano Letters, 22(18):7467-7476.

[40]ZhangAQ, LieberCM, 2016. Nano-bioelectronics. Chemical Reviews, 116(1):215-257.

[41]ZhaoYL, YouSS, ZhangAQ, et al., 2019. Scalable ultrasmall three-dimensional nanowire transistor probes for intracellular recording. Nature Nanotechnology, 14(8):783-790.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE