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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.12 P.969-976


Antiviral activities of extracts from Hong Kong seaweeds

Author(s):  Hui WANG, Engchoon Vincent OOI, Put O. Ang Jr.

Affiliation(s):  Medical College, Jinan University, Guangzhou 510632, China; more

Corresponding email(s):   twanghzzm@jnu.edu.cn, put-ang@cuhk.edu.hk

Key Words:  Hong Kong seaweed, Hydroclathrus clathratus, Antiviral activity, Herpes simplex virus (HSV), Flow cytometry

Hui WANG, Engchoon Vincent OOI, Put O. Ang Jr.. Antiviral activities of extracts from Hong Kong seaweeds[J]. Journal of Zhejiang University Science B, 2008, 9(12): 969-976.

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author="Hui WANG, Engchoon Vincent OOI, Put O. Ang Jr.",
journal="Journal of Zhejiang University Science B",
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%A Put O. Ang Jr.
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T1 - Antiviral activities of extracts from Hong Kong seaweeds
A1 - Hui WANG
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A1 - Put O. Ang Jr.
J0 - Journal of Zhejiang University Science B
VL - 9
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EP - 976
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Y1 - 2008
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B0820154

We extracted six Hong Kong brown seaweed species with hot water for their antiviral properties. The cytotoxicity and antiviral activity of these extracts were tested by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenlytetrezolium bromide] method, cytopathic effect reduction assay, and plaque reduction assay. The antiviral effect was further determined by flow cytometric analysis. The results showed that most of these extracts inhibited the propagation of herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) standard strains with very low cytotoxicity to the host cells. The extracts of Hydroclathrus clathratus and Lobophora variegata showed more potential anti-HSV activities than the extracts of the other four seaweeds. They also had moderate anti- respiratory syncytial virus (RSV) activities but could not inhibit influenza A virus. Hydroclathrus clathratus was further extracted by diluted acid and alkali and the antiviral effects of the extracts were also detected. The result showed that the hot water extract contained the main carbohydrate components that exhibited the antiviral activities against various strains of HSV, including the acyclovir-resistant strain. HI-3, a compound fractionated from this hot water extract, showed a dose-dependent anti-HSV activity in flow cytometric analysis and plaque reduction assay.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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2010-08-09 13:12:27

this artical is good

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