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CLC number: Q27; Q692

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Received: 2008-07-15

Revision Accepted: 2008-12-12

Crosschecked: 2009-03-03

Cited: 5

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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.4 P.285-290


Response enhancement of olfactory sensory neurons-based biosensors for odorant detection

Author(s):  Chun-sheng WU, Pei-hua CHEN, Qing YUAN, Ping WANG

Affiliation(s):  Biosensor National Special Laboratory, MOE Key Laboratory of Biomedical Engineering, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   cnpwang@zju.edu.cn

Key Words:  Olfactory sensory neurons (OSNs), Response enhancement, Light addressable potentiometric sensor (LAPS), Olfactory-based biosensor

Chun-sheng WU, Pei-hua CHEN, Qing YUAN, Ping WANG. Response enhancement of olfactory sensory neurons-based biosensors for odorant detection[J]. Journal of Zhejiang University Science B, 2009, 10(4): 285-290.

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author="Chun-sheng WU, Pei-hua CHEN, Qing YUAN, Ping WANG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Response enhancement of olfactory sensory neurons-based biosensors for odorant detection
%A Chun-sheng WU
%A Pei-hua CHEN
%A Qing YUAN
%A Ping WANG
%J Journal of Zhejiang University SCIENCE B
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%P 285-290
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820220

T1 - Response enhancement of olfactory sensory neurons-based biosensors for odorant detection
A1 - Chun-sheng WU
A1 - Pei-hua CHEN
A1 - Qing YUAN
A1 - Ping WANG
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 4
SP - 285
EP - 290
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820220

This paper presents a novel strategy for the response enhancement of olfactory sensory neurons (OSNs)-based biosensors by monitoring the enhancive responses of OSNs to odorants. An OSNs-based biosensor was developed on the basis of the light addressable potentiometric sensor (LAPS), in which rat OSNs were cultured on the surface of LAPS chip and served as sensing elements. LY294002, the specific inhibitor of phosphatidylinositol 3-kinase (PI3K), was used to enhance the responses of OSNs to odorants. The responses of OSNs to odorants with and without the treatment of LY294002 were recorded by LAPS. The results show that the enhancive effect of LY294002 was recorded efficiently by LAPS and the responses of this OSNs-LAPS hybrid biosensor were enhanced by LY294002 by about 1.5-fold. We conclude that this method can enhance the responses of OSNs-LAPS hybrid biosensors, which may provide a novel strategy for the bioelectrical signal monitor of OSNs in biosensors. It is also suggested that this strategy may be applicable to other kinds of OSNs-based biosensors for cellular activity detection, such as microelectrode array (MEA) and field effect transistor (FET).

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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