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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.4 P.264-272


Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29

Author(s):  Jing LI, Qian YANG, Li-hua ZHAO, Shu-mei ZHANG, Yu-xia WANG, Xiao-yu ZHAO

Affiliation(s):  Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, China; more

Corresponding email(s):   yangq@hit.edu.cn

Key Words:  Bacillus subtilis, Antifungal protein, Purification

Jing LI, Qian YANG, Li-hua ZHAO, Shu-mei ZHANG, Yu-xia WANG, Xiao-yu ZHAO. Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29[J]. Journal of Zhejiang University Science B, 2009, 10(4): 264-272.

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author="Jing LI, Qian YANG, Li-hua ZHAO, Shu-mei ZHANG, Yu-xia WANG, Xiao-yu ZHAO",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29
%A Jing LI
%A Qian YANG
%A Li-hua ZHAO
%A Shu-mei ZHANG
%A Yu-xia WANG
%A Xiao-yu ZHAO
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 4
%P 264-272
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820341

T1 - Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29
A1 - Jing LI
A1 - Qian YANG
A1 - Li-hua ZHAO
A1 - Shu-mei ZHANG
A1 - Yu-xia WANG
A1 - Xiao-yu ZHAO
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 4
SP - 264
EP - 272
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820341

An antifungal protein was isolated from a culture of Bacillus subtilis strain B29. The isolation procedure comprised ion exchange chromatography on diethylaminoethyl (DEAE)-52 cellulose and gel filtration chromatography on Bio-Gel® P-100. The protein was absorbed on DEAE-cellulose and Bio-Gel® P-100. The purified antifungal fraction was designated as B29I, with a molecular mass of 42.3 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), pI value 5.69 by isoelectric focusing (IEF)-PAGE, and 97.81% purity by high performance liquid chromatography (HPLC). B29I exhibited inhibitory activity on mycelial growth in Fusarium oxysporum, Rhizoctonia solani, Fusarium moniliforme, and Sclerotinia sclerotiorum. The 50% inhibitory concentrations (IC50) of its antifungal activity toward Fusarium oxysporum and Rhizoctonia solani were 45 and 112 μmol/L, respectively. B29I also demonstrated an inhibitory effect on conidial spore germination of Fusarium oxysporum and suppression of germ-tube elongation, and induced distortion, tumescence, and rupture of a portion of the germinated spores.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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