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CLC number: Q78

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Received: 2008-07-05

Revision Accepted: 2008-10-11

Crosschecked: 2008-10-28

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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.12 P.944-952

http://doi.org/10.1631/jzus.B0820209


Functional characterization of a potassium transporter gene NrHAK1 in Nicotiana rustica


Author(s):  Zhao-kui GUO, Qian YANG, Xiu-qing WAN, Pei-qiang YAN

Affiliation(s):  Department of Life Sciences and Engineering, Harbin Institute of Technology, Harbin 150001, China; more

Corresponding email(s):   yangq@hit.edu.cn

Key Words:  Functional characterization, K+ transporter, Nicotiana rustica


Zhao-kui GUO, Qian YANG, Xiu-qing WAN, Pei-qiang YAN. Functional characterization of a potassium transporter gene NrHAK1 in Nicotiana rustica[J]. Journal of Zhejiang University Science B, 2008, 9(12): 944-952.

@article{title="Functional characterization of a potassium transporter gene NrHAK1 in Nicotiana rustica",
author="Zhao-kui GUO, Qian YANG, Xiu-qing WAN, Pei-qiang YAN",
journal="Journal of Zhejiang University Science B",
volume="9",
number="12",
pages="944-952",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820209"
}

%0 Journal Article
%T Functional characterization of a potassium transporter gene NrHAK1 in Nicotiana rustica
%A Zhao-kui GUO
%A Qian YANG
%A Xiu-qing WAN
%A Pei-qiang YAN
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 12
%P 944-952
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820209

TY - JOUR
T1 - Functional characterization of a potassium transporter gene NrHAK1 in Nicotiana rustica
A1 - Zhao-kui GUO
A1 - Qian YANG
A1 - Xiu-qing WAN
A1 - Pei-qiang YAN
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 12
SP - 944
EP - 952
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820209


Abstract: 
The purpose of this study is to investigate the function of a novel potassium transporter gene (NrHAK1) isolated from Nicotiana rustica roots using yeast complement and real-time PCR technique. The complementary DNA (cDNA) of NrHAK1, 2 488 bp long, contains an open reading frame (ORF) of 2 334 bp encoding a protein of 777 amino acids (87.6 kDa) with 12 predicted transmembrane domains. The NrHAK1 protein shows a high sequence similarity to those of high-affinity potassium transporters in Mesembryanthemum, Phytolacca acinosa, Arabidopsis thaliana, and so on. We found that the NrHAK1 gene could complement the yeast-mutant defect in K+ uptake. Among several tissues surveyed, the expression level of NrHAK1 was most abundant in the root tip and was up-regulated when exposed to potassium starvation. Moreover, the transcript accumulation was significantly reduced by adding 5 mmol/L NH4+ to the solution. These results suggest that NrHAK1 plays an important role in potassium absorption in N. rustica.

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