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CLC number: Q81

On-line Access: 2010-03-29

Received: 2009-09-30

Revision Accepted: 2010-01-14

Crosschecked: 2010-03-02

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.4 P.292-297


A facile approach to construct hybrid multi-shell calcium phosphate gene particles

Author(s):  Zhi-xue Xu, Ran Zhang, You-xiang Wang, Qiao-ling Hu

Affiliation(s):  MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   yx_wang@zju.edu.cn, huql@zju.edu.cn

Key Words:  Calcium phosphate, Polyethylenimine (PEI), Hybrid, Gene

Zhi-xue Xu, Ran Zhang, You-xiang Wang, Qiao-ling Hu. A facile approach to construct hybrid multi-shell calcium phosphate gene particles[J]. Journal of Zhejiang University Science B, 2010, 11(4): 292-297.

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author="Zhi-xue Xu, Ran Zhang, You-xiang Wang, Qiao-ling Hu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T A facile approach to construct hybrid multi-shell calcium phosphate gene particles
%A Zhi-xue Xu
%A Ran Zhang
%A You-xiang Wang
%A Qiao-ling Hu
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 4
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%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900305

T1 - A facile approach to construct hybrid multi-shell calcium phosphate gene particles
A1 - Zhi-xue Xu
A1 - Ran Zhang
A1 - You-xiang Wang
A1 - Qiao-ling Hu
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 4
SP - 292
EP - 297
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900305

The calcium phosphate (CaP) particles have attracted much attention in gene therapy. How to construct stable gene particles was the determining factor. In this study, hybrid multi-shell CaP gene particles were successfully constructed. First, CaP nanoparticles served as a core and were coated with DNA for colloidal stabilization. The ξ-potential of DNA-coated CaP nanoparticles was −15 mV. Then polyethylenimine (PEI) was added and adsorbed outside of the DNA layer due to the electrostatic attraction. The ξ-potential of hybrid multi-shell CaP particles was slightly positive. With addition of PEI, the hybrid multi-shell particles could condense DNA effectively, which was determined by ethidium bromide (EtBr) exclusion assay. The hybrid particles were spherical and uniform with diameters of about 150 nm at proper conditions. By simple modification of PEI, the hybrid multi-shell CaP gene particles were successfully constructed. They may have great potential in gene therapy.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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