Full Text:   <2701>

CLC number: Q81

On-line Access: 2010-03-29

Received: 2009-09-30

Revision Accepted: 2010-01-14

Crosschecked: 2010-03-02

Cited: 2

Clicked: 6527

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.4 P.292-297


A facile approach to construct hybrid multi-shell calcium phosphate gene particles

Author(s):  Zhi-xue Xu, Ran Zhang, You-xiang Wang, Qiao-ling Hu

Affiliation(s):  MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   yx_wang@zju.edu.cn, huql@zju.edu.cn

Key Words:  Calcium phosphate, Polyethylenimine (PEI), Hybrid, Gene

Zhi-xue Xu, Ran Zhang, You-xiang Wang, Qiao-ling Hu. A facile approach to construct hybrid multi-shell calcium phosphate gene particles[J]. Journal of Zhejiang University Science B, 2010, 11(4): 292-297.

@article{title="A facile approach to construct hybrid multi-shell calcium phosphate gene particles",
author="Zhi-xue Xu, Ran Zhang, You-xiang Wang, Qiao-ling Hu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T A facile approach to construct hybrid multi-shell calcium phosphate gene particles
%A Zhi-xue Xu
%A Ran Zhang
%A You-xiang Wang
%A Qiao-ling Hu
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 4
%P 292-297
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900305

T1 - A facile approach to construct hybrid multi-shell calcium phosphate gene particles
A1 - Zhi-xue Xu
A1 - Ran Zhang
A1 - You-xiang Wang
A1 - Qiao-ling Hu
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 4
SP - 292
EP - 297
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900305

The calcium phosphate (CaP) particles have attracted much attention in gene therapy. How to construct stable gene particles was the determining factor. In this study, hybrid multi-shell CaP gene particles were successfully constructed. First, CaP nanoparticles served as a core and were coated with DNA for colloidal stabilization. The ξ-potential of DNA-coated CaP nanoparticles was −15 mV. Then polyethylenimine (PEI) was added and adsorbed outside of the DNA layer due to the electrostatic attraction. The ξ-potential of hybrid multi-shell CaP particles was slightly positive. With addition of PEI, the hybrid multi-shell particles could condense DNA effectively, which was determined by ethidium bromide (EtBr) exclusion assay. The hybrid particles were spherical and uniform with diameters of about 150 nm at proper conditions. By simple modification of PEI, the hybrid multi-shell CaP gene particles were successfully constructed. They may have great potential in gene therapy.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Bausinger, R., Gersdorff, K.V., Braeckmans, K., Ogris, M., Wagner, E., Brauchle, C., Zumbusch, A., 2006. The transport of nanosized gene carriers unraveled by live-cell imaging. Angew. Chem. Int. Ed., 45(10):1568-1572.

[2]Bisht, S., Bhakta, G., Mitra, S., Maitra, A., 2005. DNA loaded calcium phosphate nanoparticles: highly efficient non-viral vector for gene delivery. Int. J. Pharm., 288(1):157-168.

[3]Dutton, M.D., Varhol, R.J., Dixon, D.G., 1995. Technical considerations for the use of ethidium bromide in the quantitative analysis of nucleic acids. Anal. Biochem., 230(2):353-355.

[4]El-Aneed, A., 2004. An overview of current delivery systems in cancer gene therapy. J. Controlled Release, 94(1):1-14.

[5]Fischer, D., Bieber, T., Lin, Y.X., Elsasser, H.P., Kissel, T., 1999. A novel non-viral vector for DNA delivery based on low molecular weight, branched polyethylenimine: effect of molecular weight on transfection efficiency and cytotoxicity. Pharm. Res., 16(8):1273-1279.

[6]Kakizawa, Y., Miyata, K., Furukawa, S., Kataoka, K., 2004. Size-controlled formation of a calcium phosphate-based organic-inorganic hybrid vector for gene delivery using poly(ethylene glycol)-block-poly(aspartic acid). Adv. Mater., 16(8):699-702.

[7]Kakizawa, Y., Furukawa, S., Ishii, A., Kataoka, K., 2006. Organic-inorganic hybrid-nanocarrier of siRNA constructing through the self-assembly of calcium phosphate and PEG-based block aniomer. J. Controlled Release, 111(3):368-370.

[8]Lungwitz, U., Breunig, M., Blunk, T., Gopferich, A., 2005. Polyethylenimine-based non-viral gene delivery systems. Eur. J. Pharm. Biopharm., 60(2):247-266.

[9]Okazaki, M., Yoshida, Y., Yamaguchi, S., Kaneno, M., Elliott, J.C., 2001. Affinity binding phenomena of DNA onto apatite crystals. Biomaterials, 22(18):2459-2464.

[10]Petersen, H., Kunath, K., Martin, A.L., Stolnik, S., Roberts, C.J., Davies, M.C., Kissel, T., 2002. Star-shaped poly (ethyleneglycol)-block-polyethylenimine copolymers enhance DNA condensation of low molecular weight polyethylenimines. Biomacromolecules, 3(5):926-936.

[11]Radler, J.Q., Koltover, J., Salditt, T., 1997. Structure of DNA-cationic liposome complexes: DNA intercalation in multilamellar membranes in distinct interhelical packing regimes. Science, 275(5301):810-814.

[12]Ren, K.F., Ji, J., Shen, J.C., 2005a. Construction of polycation-based non-viral DNA nanoparticles and polyanion multilayers via layer-by-layer self-assembly. Macromol. Rapid Commun., 26(20):1633-1638.

[13]Ren, K.F., Wang, Y.X., Ji, J., Lin, Q.K., Shen, J.C., 2005b. Construction and deconstruction of PLL/DNA multilayered films for DNA delivery: effect of ionic strength. Colloids Surf. B: Biointerfaces, 46(2):63-69.

[14]Ren, K.F., Ji, J., Shen, J.C., 2006. Tunable DNA release from cross-linked ultrathin DNA/PLL multilayered films. Bioconjugate Chem., 17(1):77-83.

[15]Roy, I., Mitra, S., Maitra, A., Mozumdar, S., 2003. Calcium phosphate nanoparticles as novel non-viral vectors for targeted gene delivery. Int. J Pharm., 250(1):25-33.

[16]Sharma, V.K., Thomas, M., Klibanov, A.M., 2005. Mechanistic studies on aggregation of polyethylenimine-DNA complexes and its prevention. Biotechnol. Bioeng., 90(5):614-620.

[17]Sokolova, V., Epple, M., 2008. Inorganic nanoparticles as carriers of nucleic acids into cells. Angew. Chem. Int. Ed., 47(8):1382-1395.

[18]Sokolova, V., Radtke, I., Heumann, R., Epple, M., 2006. Effective transfection of cells with multi-shell calcium phosphate-DNA nanoparticles. Biomaterials, 27(16):3147-3153.

[19]Sokolova, V., Kovtun, A., Prymak, O., Zaika, W.M., Kubareva, E.A., Romanova, E.A., Oretskaya, T.S., Heumann, R., Epple, M., 2007. Functionalisation of calcium phosphate nanoparticles by oligonucleotides and their application for gene silencing. J Mater. Chem., 17(8):721-727.

[20]Thomas, M., Klibanov, A.M., 2002. Enhancing polyethylenimine’s delivery of plasmid DNA into mammalian cells. Proc. Natl. Acad. Sci. USA, 99(23):14640-14645.

[21]Uherek, C., Wels, W., 2000. DNA-carrier proteins for targeted gene delivery. Adv. Drug Deliv. Rev., 44(2-3):153-166.

[22]Wagner, E., Kloeckner, J., 2006. Gene delivery using polymer therapeutics. Adv. Polym. Sci., 192:135-173.

[23]Wang, Y.X., Shen, J.C., 2005. Progress in non-viral gene delivery systems fabricated via supramolecular assembly. Chin. Sci. Bull., 50(4):289-294.

[24]Wang, Y.X., Chen, P., Shen, J.C., 2006. The development and characterization of a glutathione-sensitive cross-linked polyethylenimine gene vector. Biomaterials, 27(30):5292-5298.

[25]Wang, Y.X., Chen, P., Shen, J.C., 2007. A facile entrapment approach to construct PEGylated polyplexes for improving stability in physiological condition. Colloids Surf. B: Biointerfaces, 58(2):188-196.

[26]Welzel, T., Radtke, I., Meyer-Zaika, W., Heumann, R., Epple, M., 2004. Transfection of cells with custom-made calcium phosphate nanoparticles coated with DNA. J. Mater. Chem., 14(14):2213-2217.

[27]Zhang, Y., Kohler, N., Zhang, M., 2002. Surface modification of superparamagnetic magnetite nanoparticles and their intracellular uptake. Biomaterials, 23(7):1553-1561.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE