CLC number: Q943.2
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2010-09-28
Cited: 6
Clicked: 6582
Qing-bin Wang, Wen Xu, Qing-zhong Xue, Wei-ai Su. Transgenic Brassica chinensis plants expressing a bacterial codA gene exhibit enhanced tolerance to extreme temperature and high salinity[J]. Journal of Zhejiang University Science B, 2010, 11(11): 851-861.
@article{title="Transgenic Brassica chinensis plants expressing a bacterial codA gene exhibit enhanced tolerance to extreme temperature and high salinity",
author="Qing-bin Wang, Wen Xu, Qing-zhong Xue, Wei-ai Su",
journal="Journal of Zhejiang University Science B",
volume="11",
number="11",
pages="851-861",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000137"
}
%0 Journal Article
%T Transgenic Brassica chinensis plants expressing a bacterial codA gene exhibit enhanced tolerance to extreme temperature and high salinity
%A Qing-bin Wang
%A Wen Xu
%A Qing-zhong Xue
%A Wei-ai Su
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 11
%P 851-861
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000137
TY - JOUR
T1 - Transgenic Brassica chinensis plants expressing a bacterial codA gene exhibit enhanced tolerance to extreme temperature and high salinity
A1 - Qing-bin Wang
A1 - Wen Xu
A1 - Qing-zhong Xue
A1 - Wei-ai Su
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 11
SP - 851
EP - 861
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000137
Abstract: Transgenic Brassica compestris L. spp. chinensis plants expressing a choline oxidase (codA) gene from Arthrobacter globiformis were obtained through Agrobacterium tumefaciens-mediated transformation. In the transgenic plants, codA gene expression and its product transportation to chloroplasts were detected by the enzyme-linked immunosorbent assay (ELISA) examination, immunogold localization, and 1H-nuclear magnetic resonance (1H-NMR). stress tolerance was evaluated in the T3 plants under extreme temperature and salinity conditions. The plants of transgenic line 1 (L1) showed significantly higher net photosynthetic rate (Pn) and Pn recovery rate under high (45 °C, 4 h) and low temperature (1 °C, 48 h) treatments, and higher photosynthetic rate under high salinity conditions (100, 200, and 300 mmol/L NaCl, respectively) than the wild-type plants. The enhanced tolerance to high temperature and high salinity stresses in transgenic plants is associated with the accumulation of betaine, which is not found in the wild-type plants. Our results indicate that the introduction of codA gene from Arthrobacter globiformis into Brassica compestris L. spp. chinensis could be a potential strategy for improving the plant tolerance to multiple stresses.
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