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On-line Access: 2011-03-09

Received: 2010-05-03

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.3 P.219-225

10.1631/jzus.B1000165


Potential use of cucumber (Cucumis sativus L.) endophytic fungi as seed treatment agents against root-knot nematode Meloidogyne incognita


Author(s):  Xiao-ning Yan, Richard A. Sikora, Jing-wu Zheng

Affiliation(s):  Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China, Soil-Ecosystem Phytopathology and Nematology, Institute for Resource Conservation and Plant Science (INRES), Department of Plant Health, University of Bonn, Bonn 53115, Germany, Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China

Corresponding email(s):   jwzheng@zju.edu.cn

Key Words:  Cucumber endophytic fungi, Meloidogyne incognita, Control efficacy, Colonization, In vitro activity


Xiao-ning Yan, Richard A. Sikora, Jing-wu Zheng. Potential use of cucumber (Cucumis sativus L.) endophytic fungi as seed treatment agents against root-knot nematode Meloidogyne incognita[J]. Journal of Zhejiang University Science B, 2011, 12(3): 219-225.

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author="Xiao-ning Yan, Richard A. Sikora, Jing-wu Zheng",
journal="Journal of Zhejiang University Science B",
volume="12",
number="3",
pages="219-225",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000165"
}

%0 Journal Article
%T Potential use of cucumber (Cucumis sativus L.) endophytic fungi as seed treatment agents against root-knot nematode Meloidogyne incognita
%A Xiao-ning Yan
%A Richard A. Sikora
%A Jing-wu Zheng
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 3
%P 219-225
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000165

TY - JOUR
T1 - Potential use of cucumber (Cucumis sativus L.) endophytic fungi as seed treatment agents against root-knot nematode Meloidogyne incognita
A1 - Xiao-ning Yan
A1 - Richard A. Sikora
A1 - Jing-wu Zheng
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 3
SP - 219
EP - 225
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000165


Abstract: 
Seed treatment with endophytic fungi has been regarded as an effective method for plant parasitic nematode control. Endophytic fungi from cucumber seedlings were isolated and screened for their potential to be used as seed treatment agents against Meloidogyne incognita. Among the 294 isolates screened, 23 significantly reduced galls formed by M. incognita in greenhouse test. The 10 most effective isolates were Fusarium (5), Trichoderma (1), Chaetomium (1), Acremonium (1), Paecilomyces (1), and Phyllosticta (1). Their control efficacies were repeatedly tested and their colonizations as well as in vitro activity against M. incognita were studied. They reduced the number of galls by 24.0%–58.4% in the first screening and 15.6%–44.3% in the repeated test, respectively. Phyllosticta Ph511 and Chaetomium Ch1001 had high colonizations on both the roots and the aboveground parts of cucumber seedlings. Fusarium isolates had colonization preference on the roots, their root colonizations ranging from 20.1% to 47.3% of the total root area. Trichoderma Tr882, Paecilomyces Pa972, and Acremonium Ac985 had low colonizations on both the roots and the aboveground parts. Acremonium Ac985, Chaetomium Ch1001, Paecilomyces Pa972, and Phyllosticta Ph511 produced compounds affecting motility of the second stage juveniles of M. incognita. Based on these results, Chaetomium Ch1001 was considered to have the highest potential as a seed treatment agent for M. incognita biocontrol.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Abott, W.S., 1925. A method of computing the effectiveness of an insecticide. J. Econ. Entomol., 18(6):265-267.

[2]Arnold, A.E., Mejía, L.C., Kyllo, D., Rojas, E.I., Robbins, N., Herre, E.A., 2003. Fungal endophytes limit pathogen damage in a tropical tree. PNAS, 100(26):15649-15654.

[3]Athman, S.Y., 2006. Host-Endophyte-Pest Interactions of Endophytic Fusarium oxysporum Antagonistic to Radopholus similis in Banana (Musa spp.). PhD Thesis, University of Pretoria, Pretoria, South Africa, p.25-29.

[4]Bacetty, A.A., Snook, M.E., Glenn, A.E., 2009. Toxicity of endophyte-infected tall fescue alkaloids and grass metabolites on Pratylenchus scriberiri. Phytopathology, 99(12):1336-1345.

[5]Dababat, A.A., Sikora, R.A., 2007a. Induced resistance by the mutualistic endophyte, Fusarium oxysporum 162, toward Meloidogyne incognita on tomato. Biocontrol Sci. Technol., 17(9):969-975.

[6]Dababat, A.A., Sikora, R.A., 2007b. Influence of the mutualistic endophyte Fusarium oxysporum 162 on Meloidogyne incognita attraction and invasion. Nematology, 9(6):771-776.

[7]Dababat, A.A., Selim, M.E., Saleh, A.A., Sikora, R.A., 2008. Influence of Fusarium wilt resistant tomato cultivars on root colonization of the mutualistic endophyte Fusarium oxysporum strain 162 and its biological control efficacy toward the root-knot nematode Meloidogyne incognita. J. Plant Dis. Prot., 115(6):273-278.

[8]Domsch, K.H., Gams, W., Anderson, T.H., 1980. Compendium of Soil Fungi. Academic Press, London, the United Kingdom, p.1-405.

[9]Fisher, P.J., Graf, F., Petrinil, L.E., Sutton, B.C., Wookey, P.A., 1995. Fungal endophytes of Dryas octopetala from a high polar semidesert and from the Swiss Alps. Mycologia, 87(3):319-323.

[10]Gallery, R.A., Dalling, J.W., Arnold, A.E., 2007. Diversity, host affinity and distribution of seed-infecting fungi: a case study with Cecropia. Ecology, 88(3):582-588.

[11]Goswamia, J., Pandey, R.K., Tewaria, J.P., Goswami, B.K., 2008. Management of root knot nematode on tomato through application of fungal antagonists, Acremonium strictum and Trichoderma harzianum. J. Environ. Sci. Health B, 43(3):237-240.

[12]Hallmann, J., Sikora, R.A., 1994. Influence of Fusarium oxysporum, a mutualistic fungal endophyte on Meloidogyne incognita infection of tomato. J. Plant Dis. Prot., 101(4):475-481.

[13]Huang, X.L., Xin, M.X., 2008. Experimental Technique in Microbiology. Higher Education Press, Beijing, p.55-79 (in Chinese).

[14]Hussey, R.S., Barker, K.R., 1973. A comparison of methods of collecting inocula Meloidogyne spp. including a new technique. Plant Dis. Rep., 57(6):1025-1028.

[15]Kalele, D.N., Affokpon, A., Coosemans, J., 2007. Efficacy of Paecilomyces lilacinus strain 251 against root knot nematodes in tomato under greenhouse conditions. Commun. Agric. Appl. Biol. Sci., 72(1):209-213.

[16]Kiewnick, S., Sikora, R.A., 2006. Biological control of the root-knot nematode Meloidogyne incognita by Paecilomyces lilacinus strain 251. Biol. Control, 38(2):179-187.

[17]Le, T.H., Padgham, L.J., Sikora, R.A., 2009. Biological control of the rice root-knot nematode Meloidogyne graminicola on rice, using endophytic and rhizosphere fungi. Int. J. Pest Manage., 55(1):31-36.

[18]Liao, J., Jiang, H., Sun, L., 2003. Identification of species and race of root-knot nematodes on crops in southern China. J. Huazhong Agric. Univ., 22(6):35-40 (in Chinese).

[19]Meyer, S.L.F., Huettel, R.N., Liu, X.Z., Humber, R.A., Juba, J., Nitao, J.K., 2004. Activity of fungal culture filtrates against soybean cyst nematode and root-knot nematode egg hatch and juvenlile motility. Nematology, 6(1):23-32.

[20]Mu, L.X., Niu, Y.C., Deng, H., 2010. The endophytic mycobiota in summer growing cucumber in Beijing. Mycosystema, 29(2):214-221 (in Chinese).

[21]Niere, B.I., 2001. Significance of Non-pathogenic Isolates of Fusarium oxysporum Schlecht: Fries for the Biological Control of the Burrowing Nematode Radopholus similis (Cobb) Thorne on Tissue Cultured Banana. PhD Thesis, University of Bonn, Bonn, Germany, p.45-58.

[22]Nitao, J.K., Meyer, S.L.F., Oliver, J.E., Schmidt, W.F., Chitwood, D.J., 2002. Isolation of flavipin, a fungus compound antagonistic to plant-parasitic nematodes. Nematology, 4(1):55-63.

[23]Sánchez Márquez, S., Bills, G.F., Zabalgogeazcoa, I., 2007. The endophytic mycobiota of the grass Dactylis glomerata. Fungal Diversity, 27(3):171-195.

[24]Sharon, E., Bar-Eyal, M., Chet, I., Herrera-Estrella, A., Kleifeld, O., Spiegel, Y., 2001. Biological control of the root-knot nematode Meloidogyne javanica by Trichoderma harzianum. Phytopathology, 91(7):687-693.

[25]Sikora, R.A., Fernández, E., 2005. Nematode Parasites of Vegetables. In: Luc, M., Sikora, R.A., Bridge, J. (Eds.), Plant Parasitic Nematodes in Subtropical and Tropical Agriculture. CABI, Wallingford, p.319-392.

[26]Sikora, R.A., Pocasangre, L., Zum Felde, A., Niere, B., Vu, T.T., Dababat, A.A., 2008. Mutualistic endophytic fungi and in-plant suppressiveness to plant parasitic nematodes. Biol. Control, 46(1):15-23.

[27]Stone, J.K., Bacon, C.W., White, J.F., 2000. An Overview of Endophytic Microbes: Endophytism Defined. In: Bacon, C.W., White, J.F.Jr. (Eds.), Microbial Endophytes. Marcel Dekker Inc., New York, p.3-29.

[28]Vu, T.T., Hauschild, R., Sikora, R.A., 2006. Fusarium oxysporum endophytes induced systemic resistance against Radopholus similis on banana. Nematology, 8(6):847-852.

[29]Wang, L., Yang, B., Li, C., 2001. Investigation of root-knot nematodes in East China. Forest Res., 14(5):24-28 (in Chinese).

[30]Wei, J., 1979. Fungi Identification Manual Book. Shanghai Science and Technology Press, Shanghai, p.256-461 (in Chinese).

[31]West, C.P., Oosterhuis, D.M., Robbins, R.T., 1988. The effect of Acremonium coenophialum on growth and nematode infestation of tall fescue. Plant Soil, 112(1):3-6.

[32]Zabalgogeazcoa, I., 2008. Fungal endophytes and their interaction with plant pathogens. Span. J. Agric. Res., 6(Special Issue):138-146.

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