CLC number: R87
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2014-08-15
Cited: 8
Clicked: 7547
Ying-li Lu, Wen Jing, Lian-shi Feng, Li Zhang, Jian-fang Xu, Tong-jian You, Jing Zhao. Effects of hypoxic exercise training on microRNA expression and lipid metabolism in obese rat livers[J]. Journal of Zhejiang University Science B, 2014, 15(9): 820-829.
@article{title="Effects of hypoxic exercise training on microRNA expression and lipid metabolism in obese rat livers",
author="Ying-li Lu, Wen Jing, Lian-shi Feng, Li Zhang, Jian-fang Xu, Tong-jian You, Jing Zhao",
journal="Journal of Zhejiang University Science B",
volume="15",
number="9",
pages="820-829",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400052"
}
%0 Journal Article
%T Effects of hypoxic exercise training on microRNA expression and lipid metabolism in obese rat livers
%A Ying-li Lu
%A Wen Jing
%A Lian-shi Feng
%A Li Zhang
%A Jian-fang Xu
%A Tong-jian You
%A Jing Zhao
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 9
%P 820-829
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400052
TY - JOUR
T1 - Effects of hypoxic exercise training on microRNA expression and lipid metabolism in obese rat livers
A1 - Ying-li Lu
A1 - Wen Jing
A1 - Lian-shi Feng
A1 - Li Zhang
A1 - Jian-fang Xu
A1 - Tong-jian You
A1 - Jing Zhao
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 9
SP - 820
EP - 829
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400052
Abstract: To investigate the effects of hypoxic exercise training on microRNA (miRNA) expression and the role of miRNA expression in regulating lipid metabolism, 20 dietary-induced obese SD rats were divided into a normoxic sedentary group (N, n=10) and a hypoxic exercise training group (H, n=10). After four weeks, measurements were taken of body weight, body length, fat mass, serum lipid concentration, miRNAs differentially expressed in rat liver, and gene and protein expression levels of peroxisome proliferator activated receptor α (PPARα), fatty acid synthetase (FAS), and carnitine palmitoyl transferase 1A (CPT1A) in rat liver. Body weight, Lee’s index, fat mass, fat/weight ratio, and serum levels of total cholesterol (TC) and high density lipoprotein cholesterol (HDL-C) were all significantly lower in the H group than in the N group (P<0.01). Six miRNAs expressed significantly differently in the liver (P<0.05). Specifically, expression levels of miR-378b were significantly lower in the H group than in the N group (P<0.05). Compared with the normoxic sedentary group, hypoxic exercise training resulted in a lower ratio of FAS mRNA to CPT1A mRNA (P<0.05), as well as lower CPT1A protein levels (P<0.01), while a higher ratio of FAS to CPT1A protein levels (P<0.01) was observed. In conclusion, hypoxic training may elevate the resistance of high fat diet induced obesity in rats by reducing the expression of miR-378b, and decrease the fatty acid mitochondrial oxidation in obese rat livers by decreasing the protein expression of CPT1A and increasing the protein expression ratio of FAS/CPT1A.
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