Full Text:   <1572>

Summary:  <1126>

CLC number: R965

On-line Access: 2017-06-05

Received: 2015-11-26

Revision Accepted: 2016-05-26

Crosschecked: 2017-05-26

Cited: 0

Clicked: 3163

Citations:  Bibtex RefMan EndNote GB/T7714


Betul Apaydin Yildirim


-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.6 P.501-511


Effect of Helichrysum plicatum DC. subsp. plicatum ethanol extract on gentamicin-induced nephrotoxicity in rats

Author(s):  Betul Apaydin Yildirim, Saban Kordali, Kubra Asena Terim Kapakin, Fatih Yildirim, Esra Aktas Senocak, Serdar Altun

Affiliation(s):  Department of Biochemistry, Veterinary Faculty, Ataturk University, 25240 Erzurum, Turkey; more

Corresponding email(s):   betul_apaydin@hotmail.com

Key Words:  Antioxidants, Extract, Gentamicin, Helichrysum plicatum DC. subsp. plicatum, Nephrotoxicity, Oxidative stress

Betul Apaydin Yildirim, Saban Kordali, Kubra Asena Terim Kapakin, Fatih Yildirim, Esra Aktas Senocak, Serdar Altun. Effect of Helichrysum plicatum DC. subsp. plicatum ethanol extract on gentamicin-induced nephrotoxicity in rats[J]. Journal of Zhejiang University Science B, 2017, 18(6): 501-511.

@article{title="Effect of Helichrysum plicatum DC. subsp. plicatum ethanol extract on gentamicin-induced nephrotoxicity in rats",
author="Betul Apaydin Yildirim, Saban Kordali, Kubra Asena Terim Kapakin, Fatih Yildirim, Esra Aktas Senocak, Serdar Altun",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effect of Helichrysum plicatum DC. subsp. plicatum ethanol extract on gentamicin-induced nephrotoxicity in rats
%A Betul Apaydin Yildirim
%A Saban Kordali
%A Kubra Asena Terim Kapakin
%A Fatih Yildirim
%A Esra Aktas Senocak
%A Serdar Altun
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 6
%P 501-511
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500291

T1 - Effect of Helichrysum plicatum DC. subsp. plicatum ethanol extract on gentamicin-induced nephrotoxicity in rats
A1 - Betul Apaydin Yildirim
A1 - Saban Kordali
A1 - Kubra Asena Terim Kapakin
A1 - Fatih Yildirim
A1 - Esra Aktas Senocak
A1 - Serdar Altun
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 6
SP - 501
EP - 511
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500291

The aim of this study was to evaluate the possible therapeutic or protective effects of Helichrysum plicatum DC. subsp. plicatum ethanol extract (HPE) against gentamicin-induced nephrotoxicity. Thirty-six Sprague Dawley male rats weighing between 200 and 250 g were used as live material. They were formed into six groups containing 6 rats each and were allowed to adapt to laboratory conditions for 7 d. Group I: control, 5% DMSO intraperitoneal (i.p.); Group II: HPE 100 mg/(kg·d) i.p.; Group III: HPE 200 mg/(kg·d) i.p.; Group IV: gentamicin as 80 mg/(kg·d) i.p.; Group V: gentamicin as 80 mg/(kg·d) i.p.+HPE 100 mg/(kg·d) i.p.; and Group VI: gentamicin as 80 mg/(kg·d) i.p.+HPE 200 mg/(kg·d) i.p. for 8 d. Following treatment, serum, liver, and kidney tissues were used to assess blood urea nitrogen (BUN), creatinine, enzymatic and non-enzymatic antioxidants, and lipid peroxidation. gentamicin significantly increased serum BUN, creatinin, and liver and kidney levels of malondialdehyde (MDA). It also decreased the activity of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD). Treatment with the HPE 100 mg/kg reversed gentamicin-induced alterations as evidenced by decreased serum BUN and creatinin, liver and kidney oxidant marker, and tubular necrosis as well as by an increase in antioxidant enzymes. It was found that HPE 200 mg/kg significantly increased liver and kidney tissue MDA levels in nephrotoxicity in rats. As a result, these findings support the proposition that HPE in 100 mg/kg dose demonstrates in the kidney and liver as free radicals and scavenger to prevent the toxic effects of gentamicin in both the biochemical and histopathology parameters.


方法:将36只体重200~250 g的Sprague Dawley雄性大鼠分成6组,每组6只,适应实验室条件7 d。每组处理方式不同,包括:组I,对照组,5% DMSO;组II,HPE 100 mg/(kg·d);组III,HPE 200 mg/(kg·d);组IV,庆大霉素80 mg/(kg·d);组V,庆大霉素80 mg/(kg·d)+HPE 100 mg/(kg·d);组VI,庆大霉素80 mg/(kg·d)+HPE 200 mg/(kg·d)。腹腔注射8 d后,取血清、肝和肾组织用于评估血液尿素氮(BUN)、肌酐、酶和非酶抗氧化剂和脂质过氧化。
结论:庆大霉素能显著提升血清BUN、肌酐和肝肾阳性以及丙二醛(MDA)水平,同时降低过氧化氢酶(CAT),谷胱甘肽过氧化物酶(GPx)和超氧化物歧化酶(SOD)的活性。用100 mg/kg HPE的治疗能逆转庆大霉素诱导的改变。因此,100 mg/kg HPE在肾脏和肝脏中可作为自由基和清除剂,具有缓解庆大霉素在生物化学和组织病理学上毒性的作用。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Ademiluyi, A.O., Oboh, G., Owoloye, T.R., et al., 2013. Modulatory effect of dietary inclusion of garlic (Allium Sativum) on gentamicin-induced hepatotoxicity and oxidative stress in rats. Asian. Pac. J. Trop. Biomed., 3(6): 470-475.

[2]Albayrak, S., Aksoy, A., Sagdic, O., et al., 2009. Composition, antioxidant and antimicrobial activities of Helicrysum (Asteraceae) species collected from Turkey. Food Chem., 119(1):114-122.

[3]Ali, B.H., 2003. Agents ameliorating or augmenting experimental gentamicin nephrotoxicity: some recent research. Food Chem. Toxicol., 41(11):1447-1452.

[4]Al-Kenanny, E.R., Al-Hayaly, L.K., Al-Badrany, A.G., 2012. Protective effect of Arabic gum on liver injury experimentally induced by gentamicin in mice. Kufa J. Vet. Med. Sci., 3:174-189.

[5]Aslan, M., 2000. Seker hastalıgına karsı halkilacı olarak kullanılan bitkiler üzerinde Farmakognozik Arastırmalar. Doktora tezi, Gazi Üniversitesi Saglık Bilimler Enstitüsü farmakognozik Anabilimdalı, Ankara, p.216 (in Turkish).

[6]Aslan, M., Orhan, D.D., Orhan, N., et al., 2007. In vivo antidiabetic and antioxidant potential of Helichrysum plicatum ssp. plicatum capitulums in streptozotocin-induced-diabetic rats. J. Ethnopharmacol., 109(1):54-59.

[7]Balakumar, P., Rohilla, A., Thangathirupathi, A., 2010. Gentamicin-induced nephrotoxicity: do we have a promising therapeutic approach to blunt it? Pharmacol. Res., 62(3):179-186.

[8]Banday, A.A., Farooq, N., Priyamvada, S., et al., 2008. Time dependent effects of gentamicin on the enzymes of carbohydrate metabolism, brush border membrane and oxidative stress in rat kidney tissues. Life Sci., 82(9-10): 450-459.

[9]Bayer, R.J., Breitwieaser, I., Ward, J., et al., 2007. Tribe Gnaphalieae (Cass.) Lecoq & Juillet (1831). In: Kadereit, J.W., Jeffrey, C. (Eds.), The Families and Genera of Vascular Plants, Asterales. Springer, Berlin, p.246-284.

[10]Corona, P.S., Espinal, L., Rodriguez-Pardo, D., et al., 2014. Antibiotic susceptibility in Gram-positive chronic joint arthroplasty infections: increased aminoglycoside resistance rate in patients with prior aminoglycoside-impregnated cement spacer use. J. Arthropl., 29(8):1617-1621.

[11]El-Kashef, D.H., El-Kenawi, A.E., et al., 2015. Protective effect of allicin against gentamicin-induced nephrotoxicity in rats. Int. Immunopharmacol., 29(2):679-686.

[12]Góth, L.A., 1991. Simple method for determination of serum catalase activity and revision of serum catalase activity and revision of reference range. Clin. Chim. Acta, 196(2-3):143-152.

[13]Guner, A., 2012. Turkiye Bitkileri Listesi (The List of Turkish Plants), Namas Matbaacilik San. Tic. A.Ş., Istanbul, p.163-165 (in Turkish).

[14]Kamel, M.A., Hosny Abdel Fadil, I., Noha, M.A., 2015. Prevention of Hepato-renal toxicity with vitamin E, vitamin C and their combination in gentamicin treated rats. Int. J. Pharma Sci., 5(6):1289-1296.

[15]Kandemir, F.M., Özkaraca, M., Apaydin Yildirim, B., et al., 2015. Rutin attenuates gentamicin-induced renal damage by reducing the oxidative stress, inflammation, apoptosis and autophagy in rats. Ren. Fail., 23:1-8.

[16]Liu, P., Feng, Y., Dong, D., et al., 2016. Enhanced renoprotective effect of IGF-1 modified human umbilical cord-derived mesenchymal stem cells on gentamicin-induced acute kidney injury. Sci. Rep.-UK, 6:20287.

[17]Mahmood, N., Haleh, M., Mohammad, P., et al., 2014. Pathological changes of gentamicin in liver tissue and antioxidant property of Cinnamon extract on Wistar rats. Biomed. Pharmacol. J., 7(1):341-347.

[18]Masakazu, K., Yoshiko, E., Masashi, E., 2014. Acquired resistance of Listeria monocytogenes in and escaped from liver parenchymal cells to gentamicin is caused by being coated with their plasma membrane. Microb. Infect., 16(3):237-243.

[19]Martínez-Salgado, C., López-Hernández, F.J., López-Novoa, J.M., 2007. Glomerular nephrotoxicity of amino nucleosides. Toxicol. Appl. Pharmacol., 223(1):86-98.

[20]Matkovics, B., Szabo, L., Varga, I.S., 1988. Determination of enzyme activities in lipid peroxidation and glutathione pathways. Laboratoriumi Diagnosztika, 15:248-249 (in Hungarian).

[21]Mert, N., 1996. Veteriner Klinik Biyokimya. Uludag Universitesi Veteriner Fakultesi Guclendirme Vakfı Yayın 12, Bursa, p.151-153 (in Turkish).

[22]Moreira, M.A., Nascimento, M.A., Bozzo, T.A., et al., 2014. Ascorbic acid reduces gentamicin induced nephrotoxicity in rats through the control of reactive oxygen species. Clin. Nutr., 33(2):296-301.

[23]Morone-Fortunato, I., Montemurro, C., Ruta, C., et al., 2010. Essential oils, genetic relationships and in vitro establishment of Helichrysum italicum (Roth) G. Don subsp. italicum from wild Mediterranean germplasm. Ind. Crops Prod., 32(3):639-649.

[24]Nasri, H., 2012. Acute kidney injury and beyond. J. Ren. Inj. Prev., 1(1):1-2.

[25]Nayma, S., Sadia, C.S., M Tanveer, H.P., et al., 2012. Effects of Ashwagandha (Withania somnifera) root extract on some serum liver marker enzymes (AST, ALT) in gentamicin intoxicated rats. J. Bangladesh Soc. Physiol., 7(1):1-7.

[26]Ojano-Dirain, C.P., Antonelli, P.J., le Prell, C.G., 2014. Mitochondria-targeted antioxidant MitoQ reduces gentamicin-induced ototoxicity. Otol. Neurotol., 35(3): 533-539.

[27]Ouédraogo, M., Lamien-Sanoub, A., Ramdeb, N., et al., 2013. Protective effect of Moringa oleifera leaves against gentamicin-induced nephrotoxicity in rabbits. Exp. Toxicol. Pathol., 65(3):335-339.

[28]Peng, J., Gones, G.L., Watson, K., 2000. Stress protein as biomarkers of oxidative stress: effects of antioxidant supplement. Free Radic. Biol. Med., 28(11):1598-1606.

[29]Placer, Z.A., Cushmanni, L.L., Johnson, B.C., 1966. Estimation of products of lipid peroxidation (as malondialdehyde) in biochemical systems. Anal. Biochem., 16(2): 359-364.

[30]Raju, S., Kavimani, S., Maheshwara rao, V.U., et al., 2011. Floral extract of Tecoma stans: a potent inhibitor of gentamicin-induced nephrotoxicity in vivo. Asian Pac. J. Trop. Med., 4(9):680-685.

[31]Sagdic, O., Karahan, A.G., Ozcan, M., et al., 2003. Effect of some spice extracts on bacterial inhibition. Food Sci. Technol. Int., 9(5):353-356.

[32]Sala, A., Recio, M.C., Schinella, G.R., et al., 2003. Assessment of the anti-inflammatory activity and free radical scavenger activity of tiliroside. Eur. J. Pharmacol., 461(1):53-61.

[33]Salem, E.A., Salem, N.A., Kamel, M., et al., 2010. Amelioration of gentamicin nephrotoxicity by green tea extract in uninephrectomized rats as a model of progressive renal failure. Ren. Fail., 32(10):1210-1215.

[34]Sezik, E., Yesilada, E., Honda, G., et al., 2001. Traditional medicine in Turkey. X. Folk medicine in Central Anatolia. J. Ethnopharmacol., 75(2-3):95-115.

[35]Shrestha, B., Haylor, J., 2014. Experimental rat models of chronic allograft nephropathy: a review. Int. J. Nephrol. Renovasc. Dis., 23(7):315-322.

[36]Singh, A.P., Junemann, A., Muthuraman, A., et al., 2012. Animal models of acute renal failure. Pharmacol. Rep., 64(1):31-44.

[37]Smirnov, V., Preobrazheskaia, N., Kalashnikov, I., 1982. Antibacterial properties of Helichrysum plicatum. Mikrobiol. Z., 44:71-72.

[38]Stafford, G.I., Jäger, A.K., van Staden, J., 2005. Effect of storage on the chemical composition and biological activity of several popular South African medicinal plants. J. Ethnopharmacol., 97(1):107-115.

[39]Stojiljkovic, N., Stoiljkovic, M., 2006. Micromorphological and histochemical characteristics of a rat’s liver treated with gentamicin. Acta Medica Medianae, 45(3):24-28.

[40]Sumbul, H., Gokturk, R.S., Dussen, O.D., 2003. A new endemic species of Helichrysum Gaertn. (Asteraceae-Inuleae) from south Anatolia. Bot. J. Linn. Soc., 141(2):251-254.

[41]Sun, Y., Oberley, L.W., Li, Y.A., 1988. Simple method for clinical assay of superoxide dismutase. Clin. Chem., 34(3): 497-500.

[42]Süzgec, S., Mericli, A.H., Houghton, P.J., et al., 2005. Flavonoids of Helichrysum compactum and their antioxidant and antibacterial activity. Fitoterapia, 76(2):269-272.

[43]Tavafi, M., 2013. Protection of renal tubules against gentamicin induced nephrotoxicity. J. Ren. Inj. Prev., 2(1):5-6.

[44]Tavafi, M., Ahmadvand, H., 2011. Effect of rosmarinic acid on inhibition of gentamicin induced nephrotoxicity in rats. Tissue Cell, 43(6):392-397.

[45]Veljković, M., Pavlović, D.R., Stojiljković, N., et al., 2016. Morphological and morphometric study of protective effect of green tea in gentamicin-induced nephrotoxicity in rats. Life Sci., 147:85-91.

[46]Wang, Y., Iwatani, H., Ito, T., et al., 2004. Fetal cells in mother rats contribute to the remodeling of liver and kidney after injury. Biochem. Bioph. Res. Commun., 325(3):961-967.

[47]Yaman, I., Balikci, S., 2010. Protective effect of Nigella sativa against gentamicin-induced nephrotoxicity in rats. Exp. Toxicol. Path., 62(2):183-190.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE