CLC number: R331.1; R915
On-line Access: 2017-07-05
Received: 2016-05-19
Revision Accepted: 2016-06-20
Crosschecked: 2017-06-07
Cited: 0
Clicked: 4567
Ke Hao, Bing-yu Chen, Kai-qiang Li, Yu Zhang, Cai-xia Li, Ying Wang, Lu-xi Jiang, Jiang Shen, Xiang-chai Guo, Wei Zhang, Meng-hua Zhu, Zhen Wang. Cytotoxicity of anti-tumor herbal Marsdeniae tenacissimae extract on erythrocytes[J]. Journal of Zhejiang University Science B, 2017, 18(7): 597-604.
@article{title="Cytotoxicity of anti-tumor herbal Marsdeniae tenacissimae extract on erythrocytes",
author="Ke Hao, Bing-yu Chen, Kai-qiang Li, Yu Zhang, Cai-xia Li, Ying Wang, Lu-xi Jiang, Jiang Shen, Xiang-chai Guo, Wei Zhang, Meng-hua Zhu, Zhen Wang",
journal="Journal of Zhejiang University Science B",
volume="18",
number="7",
pages="597-604",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600228"
}
%0 Journal Article
%T Cytotoxicity of anti-tumor herbal Marsdeniae tenacissimae extract on erythrocytes
%A Ke Hao
%A Bing-yu Chen
%A Kai-qiang Li
%A Yu Zhang
%A Cai-xia Li
%A Ying Wang
%A Lu-xi Jiang
%A Jiang Shen
%A Xiang-chai Guo
%A Wei Zhang
%A Meng-hua Zhu
%A Zhen Wang
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 7
%P 597-604
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600228
TY - JOUR
T1 - Cytotoxicity of anti-tumor herbal Marsdeniae tenacissimae extract on erythrocytes
A1 - Ke Hao
A1 - Bing-yu Chen
A1 - Kai-qiang Li
A1 - Yu Zhang
A1 - Cai-xia Li
A1 - Ying Wang
A1 - Lu-xi Jiang
A1 - Jiang Shen
A1 - Xiang-chai Guo
A1 - Wei Zhang
A1 - Meng-hua Zhu
A1 - Zhen Wang
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 7
SP - 597
EP - 604
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600228
Abstract: Marsdeniae tenacissimae extract (MTE) has been used as an adjuvant medicine for cancer therapy for a long time. Although massive studies demonstrated its considerable anti-cancer effect, there is no research on its influence on erythrocytes, which are firstly interacted with MTE in the circulation. To investigate the influence of MTE on erythrocytes, we used a flow cytometer to detect the MTE-treated alternations of morphology, calcium concentration, and reactive oxygen species (ROS) level in erythrocytes. We used hemolysis under different osmotic solutions to evaluate the fragility of erythrocytes. Data showed that MTE treatment dose-dependently increased the ratio of erythrocyte fragmentation (P<0.001) and shrinking, and elevated the forward scatter (FSC) value (P<0.001) and calcium accumulation (P<0.001). MTE induced ROS production of erythrocytes under the high glucose condition (P<0.01) and consequently caused a rise in fragility (P<0.05). These results suggest that MTE induces cytotoxicity and aging in erythrocytes in a dose-dependent manner, and presents the possibility of impairment on cancer patients’ circulating erythrocytes when MTE is used as an anti-cancer adjuvant medicine.
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