CLC number: Q812
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2018-07-10
Cited: 0
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Miao-Miao Liu, Xiao-Jing Zhang, Yan Gao, Zhi-Cheng Shen, Chao-Yang Lin. Molecular characterization and efficacy evaluation of a transgenic corn event for insect resistance and glyphosate tolerance[J]. Journal of Zhejiang University Science B, 2018, 19(8): 610-619.
@article{title="Molecular characterization and efficacy evaluation of a transgenic corn event for insect resistance and glyphosate tolerance",
author="Miao-Miao Liu, Xiao-Jing Zhang, Yan Gao, Zhi-Cheng Shen, Chao-Yang Lin",
journal="Journal of Zhejiang University Science B",
volume="19",
number="8",
pages="610-619",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700345"
}
%0 Journal Article
%T Molecular characterization and efficacy evaluation of a transgenic corn event for insect resistance and glyphosate tolerance
%A Miao-Miao Liu
%A Xiao-Jing Zhang
%A Yan Gao
%A Zhi-Cheng Shen
%A Chao-Yang Lin
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 8
%P 610-619
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700345
TY - JOUR
T1 - Molecular characterization and efficacy evaluation of a transgenic corn event for insect resistance and glyphosate tolerance
A1 - Miao-Miao Liu
A1 - Xiao-Jing Zhang
A1 - Yan Gao
A1 - Zhi-Cheng Shen
A1 - Chao-Yang Lin
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 8
SP - 610
EP - 619
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700345
Abstract: A transgenic maize event ZD12-6 expressing a Bacillus thuringiensis (Bt) fusion protein Cry1Ab/Cry2Aj and a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) protein G10 was characterized and evaluated. Southern blot analysis indicated that ZD12-6 is a single copy integration event. The insert site was determined to be at chromosome 1 by border sequence analysis. Expression analyses of Bt fusion protein Cry1Ab/Cry2Aj and the EPSPS protein G10 suggested that they are both expressed stably in different generations. Insect bioassays demonstrated that the transgenic plants are highly resistant to Asian corn borer (Ostrinia furnacalis), cotton boll worm (Helicoverpa armigera), and armyworm (Mythimna separata). This study suggested that ZD12-6 has the potential to be developed into a commercial transgenic line.
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