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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.8 P.581-595

http://doi.org/10.1631/jzus.B1700408


Transcriptional and translational responses of rapeseed leaves to red and blue lights at the rosette stage


Author(s):  Sheng-Xin Chang, Chu Pu, Rong-Zhan Guan, Min Pu, Zhi-Gang Xu

Affiliation(s):  College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China; more

Corresponding email(s):   xuzhigang@njau.edu.cn

Key Words:  Brassica napus L., Light emitting diode (LED) light, Comparative transcriptome and proteome, Leaf morphogenesis, Stress response


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Sheng-Xin Chang, Chu Pu, Rong-Zhan Guan, Min Pu, Zhi-Gang Xu. Transcriptional and translational responses of rapeseed leaves to red and blue lights at the rosette stage[J]. Journal of Zhejiang University Science B, 2018, 19(8): 581-595.

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author="Sheng-Xin Chang, Chu Pu, Rong-Zhan Guan, Min Pu, Zhi-Gang Xu",
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volume="19",
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doi="10.1631/jzus.B1700408"
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%T Transcriptional and translational responses of rapeseed leaves to red and blue lights at the rosette stage
%A Sheng-Xin Chang
%A Chu Pu
%A Rong-Zhan Guan
%A Min Pu
%A Zhi-Gang Xu
%J Journal of Zhejiang University SCIENCE B
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%P 581-595
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700408

TY - JOUR
T1 - Transcriptional and translational responses of rapeseed leaves to red and blue lights at the rosette stage
A1 - Sheng-Xin Chang
A1 - Chu Pu
A1 - Rong-Zhan Guan
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A1 - Zhi-Gang Xu
J0 - Journal of Zhejiang University Science B
VL - 19
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SP - 581
EP - 595
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1700408


Abstract: 
Under different red (R):blue (B) photon flux ratios, the growth performance of rapeseed (Brassica napus L.) is significantly different. Rapeseed under high R ratios shows shade response, while under high B ratios it shows sun-type morphology. Rapeseed under monochromatic red or blue light is seriously stressed. Transcriptomic and proteomic methods were used to analyze the metabolic pathway change of rapeseed (cv. “Zhongshuang 11”) leaves under different R:B photon flux ratios (including 100R:0B%, 75R:25B%, 25R:75B%, and 0R:100B%), based on digital gene expression (DGE) and two-dimensional gel electrophoresis (2-DE). For DGE analysis, 2054 differentially expressed transcripts (|log2(fold change)|≥1, q<0.005) were detected among the treatments. High R ratios (100R:0B% and 75R:25B%) enhanced the expression of cellular structural components, mainly the cell wall and cell membrane. These components participated in plant epidermis development and anatomical structure morphogenesis. This might be related to the shade response induced by red light. High B ratios (25R:75B% and 0R:100B%) promoted the expression of chloroplast-related components, which might be involved in the formation of sun-type chloroplast induced by blue light. For 2-DE analysis, 37 protein spots showed more than a 2-fold difference in expression among the treatments. Monochromatic light (ML; 100R:0B% and 0R:100B%) stimulated accumulation of proteins associated with antioxidation, photosystem II (PSII), DNA and ribosome repairs, while compound light (CL; 75R:25B% and 25R:75B%) accelerated accumulation of proteins associated with carbohydrate, nucleic acid, amino acid, vitamin, and xanthophyll metabolisms. These findings can be useful in understanding the response mechanisms of rapeseed leaves to different R:B photon flux ratios.

红蓝光质下苗期油菜基因和蛋白表达特性的研究

目的:研究不同比例红蓝光下苗期油菜表型、转录和蛋白水平的差异.
创新点:利用转录组和蛋白组技术对不同红蓝光质下油菜叶片的分子表达进行检测,并探讨了其与叶片表型响应的关系.
方法:采用数字基因表达谱和双向电泳技术检测红蓝光处理后油菜叶片的基因和蛋白表达水平,并分析处理间的差异.
结论:不同比例红蓝光下,油菜叶片转录组和蛋白组呈系统性变化.高比例红光诱发叶片表皮发育和解剖结构形态建成相关基因的表达,它们可能与高比红光诱发的遮阴应答相关.高比蓝光促进叶绿体相关基因的表达,它们可能与高比蓝光下阳生型叶绿体的形成相关.红蓝单色光诱发胁迫应答相关蛋白的表达,而红蓝复合光促进碳氮代谢和次生代谢相关蛋白的表达.

关键词:油菜;发光二极管光源;转录组和蛋白组;叶片表型;胁迫应答

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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[47]List of electronic supplementary materials

[48]Fig. S1 Major technique parameters of different light spectral energy distributions under LED

[49]Fig. S2 GO enrichment analysis of differentially expressed genes between 100R:0B% and 0R:100B%

[50]Fig. S3 GO enrichment analysis of differentially expressed genes between 75R:25B% and 25R:75B%

[51]Fig. S4 Venn analysis and GO enrichment analysis of differentially expressed genes between CL (75R:25B% and 25R:75B%) and 100R:0B%

[52]Fig. S5 Venn analysis and GO enrichment analysis of differentially expressed genes between CL (75R:25B% and 25R:75B%) and 0R:100B%

[53]Fig. S6 qRT-PCR analysis of ten random genes for the four light quality treatments

[54]Table S1 Primers of ten randomly selected differentially expressed genes

[55]Table S2 Thirty-seven proteins identified by MALDI-TOF/TOF MS in the rapeseed leaves grown under different light qualities

[56]Table S3 Genes in photomorphogenesis-related GO terms enriched between 100R:0B% and 0R:100B%

[57]Table S4 Genes in photomorphogenesis-related GO terms enriched between 75R:25B% and 25R:75B%

[58]Table S5 Genes in chloroplast-related GO terms enriched between 100R:0B% and 0R:100B%

[59]Table S6 Genes in chloroplast-related GO terms enriched between 75R:25B% and 25R:75B%

[60]File S1 Differentially expressed genes between each of two light quality treatments identified by DEGseq

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