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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.8 P.581-595

10.1631/jzus.B1700408


Transcriptional and translational responses of rapeseed leaves to red and blue lights at the rosette stage


Author(s):  Sheng-Xin Chang, Chu Pu, Rong-Zhan Guan, Min Pu, Zhi-Gang Xu

Affiliation(s):  College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China; more

Corresponding email(s):   xuzhigang@njau.edu.cn

Key Words:  Brassica napus L., Light emitting diode (LED) light, Comparative transcriptome and proteome, Leaf morphogenesis, Stress response


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Sheng-Xin Chang, Chu Pu, Rong-Zhan Guan, Min Pu, Zhi-Gang Xu. Transcriptional and translational responses of rapeseed leaves to red and blue lights at the rosette stage[J]. Journal of Zhejiang University Science B, 2018, 19(1): 581-595.

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author="Sheng-Xin Chang, Chu Pu, Rong-Zhan Guan, Min Pu, Zhi-Gang Xu",
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volume="19",
number="8",
pages="581-595",
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publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700408"
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%T Transcriptional and translational responses of rapeseed leaves to red and blue lights at the rosette stage
%A Sheng-Xin Chang
%A Chu Pu
%A Rong-Zhan Guan
%A Min Pu
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%P 581-595
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T1 - Transcriptional and translational responses of rapeseed leaves to red and blue lights at the rosette stage
A1 - Sheng-Xin Chang
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VL - 19
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Abstract: 
Under different red (R):blue (B) photon flux ratios, the growth performance of rapeseed (Brassica napus L.) is significantly different. Rapeseed under high R ratios shows shade response, while under high B ratios it shows sun-type morphology. Rapeseed under monochromatic red or blue light is seriously stressed. Transcriptomic and proteomic methods were used to analyze the metabolic pathway change of rapeseed (cv. “Zhongshuang 11”) leaves under different R:B photon flux ratios (including 100R:0B%, 75R:25B%, 25R:75B%, and 0R:100B%), based on digital gene expression (DGE) and two-dimensional gel electrophoresis (2-DE). For DGE analysis, 2054 differentially expressed transcripts (|log2(fold change)|≥1, q<0.005) were detected among the treatments. High R ratios (100R:0B% and 75R:25B%) enhanced the expression of cellular structural components, mainly the cell wall and cell membrane. These components participated in plant epidermis development and anatomical structure morphogenesis. This might be related to the shade response induced by red light. High B ratios (25R:75B% and 0R:100B%) promoted the expression of chloroplast-related components, which might be involved in the formation of sun-type chloroplast induced by blue light. For 2-DE analysis, 37 protein spots showed more than a 2-fold difference in expression among the treatments. Monochromatic light (ML; 100R:0B% and 0R:100B%) stimulated accumulation of proteins associated with antioxidation, photosystem II (PSII), DNA and ribosome repairs, while compound light (CL; 75R:25B% and 25R:75B%) accelerated accumulation of proteins associated with carbohydrate, nucleic acid, amino acid, vitamin, and xanthophyll metabolisms. These findings can be useful in understanding the response mechanisms of rapeseed leaves to different R:B photon flux ratios.

红蓝光质下苗期油菜基因和蛋白表达特性的研究

目的:研究不同比例红蓝光下苗期油菜表型、转录和蛋白水平的差异.
创新点:利用转录组和蛋白组技术对不同红蓝光质下油菜叶片的分子表达进行检测,并探讨了其与叶片表型响应的关系.
方法:采用数字基因表达谱和双向电泳技术检测红蓝光处理后油菜叶片的基因和蛋白表达水平,并分析处理间的差异.
结论:不同比例红蓝光下,油菜叶片转录组和蛋白组呈系统性变化.高比例红光诱发叶片表皮发育和解剖结构形态建成相关基因的表达,它们可能与高比红光诱发的遮阴应答相关.高比蓝光促进叶绿体相关基因的表达,它们可能与高比蓝光下阳生型叶绿体的形成相关.红蓝单色光诱发胁迫应答相关蛋白的表达,而红蓝复合光促进碳氮代谢和次生代谢相关蛋白的表达.

关键词:油菜;发光二极管光源;转录组和蛋白组;叶片表型;胁迫应答

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Adamska I, Ohad I, Kloppstech K, 1992. Synthesis of the early light-inducible protein is controlled by blue light and related to light stress. Proc Natl Acad Sci USA, 89(7):2610-2613.

[2]Anderson MB, Folta K, Warpeha KM, et al., 1999. Blue light-directed destabilization of the pea Lhcb1*4 transcript depends on sequences within the 5' untranslated region. Plant Cell, 11(8):1579-1589.

[3]Bradford MM, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 72(1-2):248-254.

[4]Buschmann C, Meier D, Kleudgen HK, et al., 1978. Regulation of chloroplast development by red and blue light. Photochem Photobiol, 27(2):195-198.

[5]Chang SX, Li CX, Yao XY, et al., 2016. Morphological, photosynthetic, and physiological responses of rapeseed leaf to different combinations of red and blue lights at the rosette stage. Front Plant Sci, 7:1144.

[6]Cosgrove DJ, 2000. Loosening of plant cell walls by expansins. Nature, 407(6802):321-326.

[7]Fan J, Chen CX, Yu QB, et al., 2011. Comparative iTRAQ proteome and transcriptome analyses of sweet orange infected by “Candidatus Liberibacter asiaticus”. Physiol Plantarum, 143(3):235-245.

[8]Gorecka KM, Konopka-Postupolska D, Hennig J, et al., 2005. Peroxidase activity of annexin 1 from Arabidopsis thaliana. Biochem Biophys Res Commun, 336(3):868-875.

[9]Hayashi S, Ishii T, Matsunaga T, et al., 2008. The glycerophosphoryl diester phosphodiesterase-like proteins SHV3 and its homologs play important roles in cell wall organization. Plant Cell Physiol, 49(10):1522-1535.

[10]Hejátko J, Ryu H, Kim GT, et al., 2009. The histidine kinases CYTOKININ-INDEPENDENT1 and ARABIDOPSIS HISTIDINE KINASE2 and 3 regulate vascular tissue development in Arabidopsis shoots. Plant Cell, 21(7):2008-2021.

[11]Hernández R, Kubota C, 2016. Physiological responses of cucumber seedlings under different blue and red photon flux ratios using LEDs. Environ Exp Bot, 121:66-74.

[12]Hogewoning SW, Trouwborst G, Maljaars H, et al., 2010. Blue light dose-responses of leaf photosynthesis, morphology, and chemical composition of Cucumis sativus grown under different combinations of red and blue light. J Exp Bot, 61(11):3107-3117.

[13]Inoue SI, Takemiya A, Shimazaki KI, 2010. Phototropin signaling and stomatal opening as a model case. Curr Opin Plant Biol, 13(5):587-593.

[14]Jungandreas A, Schellenberger Costa B, Jakob T, et al., 2014. The acclimation of Phaeodactylum tricornutum to blue and red light does not influence the photosynthetic light reaction but strongly disturbs the carbon allocation pattern. PLoS ONE, 9(8):e99727.

[15]Kasukabe Y, He LX, Nada K, et al., 2004. Overexpression of spermidine synthase enhances tolerance to multiple environmental stresses and up-regulates the expression of various stress-regulated genes in transgenic Arabidopsis thaliana. Plant Cell Physiol, 45(6):712-722.

[16]Kim DS, Cho DS, Park WM, et al., 2006. Proteomic pattern-based analyses of light responses in Arabidopsis thaliana wild-type and photoreceptor mutants. Proteomics, 6(10):3040-3049.

[17]Lan P, Li WF, Schmidt W, 2012. Complementary proteome and transcriptome profiling in phosphate-deficient Arabidopsis roots reveals multiple levels of gene regulation. Mol Cell Proteomics, 11(11):1156-1166.

[18]Li JG, Li G, Wang HY, et al., 2011. Phytochrome signaling mechanisms. Arabidopsis Book, 9:e0148.

[19]Lichtenthaler HK, Buschmann C, Rahmsdorf U, 1980. The importance of blue light for the development of sun-type chloroplasts. In: Senger H (Ed.), The Blue Light Syndrome. Springer, Berlin, Heidelberg, p.485-494.

[20]Liu HT, Liu B, Zhao CX, et al., 2011. The action mechanisms of plant cryptochromes. Trends Plant Sci, 16(12):684-691.

[21]Liu JH, Wang W, Wu H, et al., 2015. Polyamines function in stress tolerance: from synthesis to regulation. Front Plant Sci, 6(827):827.

[22]Ma LG, Li JM, Qu LJ, et al., 2001. Light control of Arabidopsis development entails coordinated regulation of genome expression and cellular pathways. Plant Cell, 13(12):2589-2607.

[23]Machado CR, Costa de Oliveira RL, Boiteux S, et al., 1996. Thi1, a thiamine biosynthetic gene in Arabidopsis thaliana, complements bacterial defects in DNA repair. Plant Mol Biol, 31(3):585-593.

[24]Marshall SDG, Putterill JJ, Plummer KM, et al., 2003. The carboxylesterase gene family from Arabidopsis thaliana. J Mol Evol, 57(5):487-500.

[25]Novikova GV, Nosov AV, Stepanchenko NS, et al., 2013. Plant cell proliferation and its regulators. Russ J Plant Physiol, 60(4):500-506.

[26]Peterman TK, Ohol YM, McReynolds LJ, et al., 2004. Patellin1, a novel Sec14-like protein, localizes to the cell plate and binds phosphoinositides. Plant Physiol, 136(2):3080-3094.

[27]Pfannschmidt T, 2003. Chloroplast redox signals: how photosynthesis controls its own genes. Trends Plant Sci, 8(1):33-41.

[28]Pi JB, Zhang Q, Fu JQ, et al., 2010. ROS signaling, oxidative stress and Nrf2 in pancreatic beta-cell function. Toxicol Appl Pharm, 244(1):77-83.

[29]Qi JN, Yu SC, Zhang FL, et al., 2010. Reference gene selection for real-time quantitative polymerase chain reaction of mRNA transcript levels in Chinese cabbage (Brassica rapa L. ssp. pekinensis). Plant Mol Biol Rep, 28(4):597-604.

[30]Roig-Villanova I, Bou J, Sorin C, et al., 2006. Identification of primary target genes of phytochrome signaling. Early transcriptional control during shade avoidance responses in Arabidopsis. Plant Physiol, 141(1):85-96.

[31]Rose JK, Saladié M, Catalá C, 2004. The plot thickens: new perspectives of primary cell wall modification. Curr Opin Plant Biol, 7(3):296-301.

[32]Roxas VP, Lodhi SA, Garrett DK, et al., 2000. Stress tolerance in transgenic tobacco seedlings that overexpress glutathione S-transferase/glutathione peroxidase. Plant Cell Physiol, 41(11):1229-1234.

[33]Schuerger AC, Brown CS, Stryjewski EC, 1997. Anatomical features of pepper plants (Capsicum annuum L.) grown under red light-emitting diodes supplemented with blue or far-red light. Ann Bot, 79(3):273-282.

[34]Singh DK, McNellis TW, 2011. Fibrillin protein function: the tip of the iceberg? Trends Plant Sci, 16(8):432-441.

[35]Tanaka H, Watanabe M, Sasabe M, et al., 2007. Novel receptor-like kinase ALE2 controls shoot development by specifying epidermis in Arabidopsis. Development, 134(9):1643-1652.

[36]Tausz M, Šircelj H, Grill D, 2004. The glutathione system as a stress marker in plant ecophysiology: is a stress-response concept valid? J Exp Bot, 55(404):1955-1962.

[37]Tsukaya H, 2002. Leaf development. Arabidopsis Book, 1:e0072.

[38]Tsukaya H, 2006. Mechanism of leaf-shape determination. Ann Rev Plant Biol, 57:477-496.

[39]Ulm R, Nagy F, 2005. Signalling and gene regulation in response to ultraviolet light. Curr Opin Plant Biol, 8(5):477-482.

[40]Wang W, Vignani R, Scali M, et al., 2006. A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis. Electrophoresis, 27(13):2782-2786.

[41]Wang WJ, Wang FJ, Sun XT, et al., 2013. Comparison of transcriptome under red and blue light culture of Saccharina japonica (Phaeophyceae). Planta, 237(4):1123-1133.

[42]Wang XW, Wang HZ, Wang J, et al., 2011. The genome of the mesopolyploid crop species Brassica rapa. Nat Genet, 43(10):1035-1039.

[43]Yang Y, Sulpice R, Himmelbach A, et al., 2006. Fibrillin expression is regulated by abscisic acid response regulators and is involved in abscisic acid-mediated photoprotection. Proc Natl Acad Sci USA, 103(15):6061-6066.

[44]Yang YJ, Li Y, Li X, et al., 2008. Comparative proteomics analysis of light responses in cryptochrome1–304 and Columbia wild-type 4 of Arabidopsis thaliana. Acta Biochim Biophys Sin, 40(1):27-37.

[45]Youssef A, Laizet Y, Block MA, et al., 2010. Plant lipid-associated fibrillin proteins condition jasmonate production under photosynthetic stress. Plant J, 61(3):436-445.

[46]Zhuang WB, Gao ZH, Wang LJ, et al., 2013. Comparative proteomic and transcriptomic approaches to address the active role of GA4 in Japanese apricot flower bud dormancy release. J Exp Bot, 64(16):4953-4966.

[47]List of electronic supplementary materials

[48]Fig. S1 Major technique parameters of different light spectral energy distributions under LED

[49]Fig. S2 GO enrichment analysis of differentially expressed genes between 100R:0B% and 0R:100B%

[50]Fig. S3 GO enrichment analysis of differentially expressed genes between 75R:25B% and 25R:75B%

[51]Fig. S4 Venn analysis and GO enrichment analysis of differentially expressed genes between CL (75R:25B% and 25R:75B%) and 100R:0B%

[52]Fig. S5 Venn analysis and GO enrichment analysis of differentially expressed genes between CL (75R:25B% and 25R:75B%) and 0R:100B%

[53]Fig. S6 qRT-PCR analysis of ten random genes for the four light quality treatments

[54]Table S1 Primers of ten randomly selected differentially expressed genes

[55]Table S2 Thirty-seven proteins identified by MALDI-TOF/TOF MS in the rapeseed leaves grown under different light qualities

[56]Table S3 Genes in photomorphogenesis-related GO terms enriched between 100R:0B% and 0R:100B%

[57]Table S4 Genes in photomorphogenesis-related GO terms enriched between 75R:25B% and 25R:75B%

[58]Table S5 Genes in chloroplast-related GO terms enriched between 100R:0B% and 0R:100B%

[59]Table S6 Genes in chloroplast-related GO terms enriched between 75R:25B% and 25R:75B%

[60]File S1 Differentially expressed genes between each of two light quality treatments identified by DEGseq

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