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Received: 2017-08-15

Revision Accepted: 2017-11-26

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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.8 P.581-595


Transcriptional and translational responses of rapeseed leaves to red and blue lights at the rosette stage

Author(s):  Sheng-Xin Chang, Chu Pu, Rong-Zhan Guan, Min Pu, Zhi-Gang Xu

Affiliation(s):  College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China; more

Corresponding email(s):   xuzhigang@njau.edu.cn

Key Words:  Brassica napus L., Light emitting diode (LED) light, Comparative transcriptome and proteome, Leaf morphogenesis, Stress response

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Sheng-Xin Chang, Chu Pu, Rong-Zhan Guan, Min Pu, Zhi-Gang Xu. Transcriptional and translational responses of rapeseed leaves to red and blue lights at the rosette stage[J]. Journal of Zhejiang University Science B, 2018, 19(8): 581-595.

@article{title="Transcriptional and translational responses of rapeseed leaves to red and blue lights at the rosette stage",
author="Sheng-Xin Chang, Chu Pu, Rong-Zhan Guan, Min Pu, Zhi-Gang Xu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Transcriptional and translational responses of rapeseed leaves to red and blue lights at the rosette stage
%A Sheng-Xin Chang
%A Chu Pu
%A Rong-Zhan Guan
%A Min Pu
%A Zhi-Gang Xu
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 8
%P 581-595
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700408

T1 - Transcriptional and translational responses of rapeseed leaves to red and blue lights at the rosette stage
A1 - Sheng-Xin Chang
A1 - Chu Pu
A1 - Rong-Zhan Guan
A1 - Min Pu
A1 - Zhi-Gang Xu
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 8
SP - 581
EP - 595
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700408

Under different red (R):blue (B) photon flux ratios, the growth performance of rapeseed (Brassica napus L.) is significantly different. Rapeseed under high R ratios shows shade response, while under high B ratios it shows sun-type morphology. Rapeseed under monochromatic red or blue light is seriously stressed. Transcriptomic and proteomic methods were used to analyze the metabolic pathway change of rapeseed (cv. “Zhongshuang 11”) leaves under different R:B photon flux ratios (including 100R:0B%, 75R:25B%, 25R:75B%, and 0R:100B%), based on digital gene expression (DGE) and two-dimensional gel electrophoresis (2-DE). For DGE analysis, 2054 differentially expressed transcripts (|log2(fold change)|≥1, q<0.005) were detected among the treatments. High R ratios (100R:0B% and 75R:25B%) enhanced the expression of cellular structural components, mainly the cell wall and cell membrane. These components participated in plant epidermis development and anatomical structure morphogenesis. This might be related to the shade response induced by red light. High B ratios (25R:75B% and 0R:100B%) promoted the expression of chloroplast-related components, which might be involved in the formation of sun-type chloroplast induced by blue light. For 2-DE analysis, 37 protein spots showed more than a 2-fold difference in expression among the treatments. Monochromatic light (ML; 100R:0B% and 0R:100B%) stimulated accumulation of proteins associated with antioxidation, photosystem II (PSII), DNA and ribosome repairs, while compound light (CL; 75R:25B% and 25R:75B%) accelerated accumulation of proteins associated with carbohydrate, nucleic acid, amino acid, vitamin, and xanthophyll metabolisms. These findings can be useful in understanding the response mechanisms of rapeseed leaves to different R:B photon flux ratios.




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[47]List of electronic supplementary materials

[48]Fig. S1 Major technique parameters of different light spectral energy distributions under LED

[49]Fig. S2 GO enrichment analysis of differentially expressed genes between 100R:0B% and 0R:100B%

[50]Fig. S3 GO enrichment analysis of differentially expressed genes between 75R:25B% and 25R:75B%

[51]Fig. S4 Venn analysis and GO enrichment analysis of differentially expressed genes between CL (75R:25B% and 25R:75B%) and 100R:0B%

[52]Fig. S5 Venn analysis and GO enrichment analysis of differentially expressed genes between CL (75R:25B% and 25R:75B%) and 0R:100B%

[53]Fig. S6 qRT-PCR analysis of ten random genes for the four light quality treatments

[54]Table S1 Primers of ten randomly selected differentially expressed genes

[55]Table S2 Thirty-seven proteins identified by MALDI-TOF/TOF MS in the rapeseed leaves grown under different light qualities

[56]Table S3 Genes in photomorphogenesis-related GO terms enriched between 100R:0B% and 0R:100B%

[57]Table S4 Genes in photomorphogenesis-related GO terms enriched between 75R:25B% and 25R:75B%

[58]Table S5 Genes in chloroplast-related GO terms enriched between 100R:0B% and 0R:100B%

[59]Table S6 Genes in chloroplast-related GO terms enriched between 75R:25B% and 25R:75B%

[60]File S1 Differentially expressed genes between each of two light quality treatments identified by DEGseq

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