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Suppl. Mater.: 

CLC number: S858.31

On-line Access: 2019-08-05

Received: 2019-02-14

Revision Accepted: 2019-05-02

Crosschecked: 2019-07-09

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Clicked: 734

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Min Liao

https://orcid.org/0000-0003-3006-9335

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Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.9 P.740-752

http://doi.org/10.1631/jzus.B1900070


Pathogenicity of an FAdV-4 isolate to chickens and its genomic analysis


Author(s):  Kai-Kun Mo, Chen-Fei Lyu, Shang-Shang Cao, Xia Li, Gang Xing, Yan Yan, Xiao-Juan Zheng, Min Liao, Ji-Yong Zhou

Affiliation(s):  Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   liaomin4545@zju.edu.cn

Key Words:  Fowl adenovirus serotype 4 (FAdV-4), Pathogenicity, Infection way, Virus neutralization activity, Genome analysis


Kai-Kun Mo, Chen-Fei Lyu, Shang-Shang Cao, Xia Li, Gang Xing, Yan Yan, Xiao-Juan Zheng, Min Liao, Ji-Yong Zhou. Pathogenicity of an FAdV-4 isolate to chickens and its genomic analysis[J]. Journal of Zhejiang University Science B, 2019, 20(9): 740-752.

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volume="20",
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pages="740-752",
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publisher="Zhejiang University Press & Springer",
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Abstract: 
fowl adenovirus serotype 4 (FAdV-4) strain SD1511 was isolated from chickens with severe inclusion body hepatitis and hydropericardium syndrome in Shandong Province, China. The isolate was cultured in primary chicken embryo kidney cells. A study of pathogenicity indicated that SD1511 readily infected 7–35-d-old chickens by intramuscular injection and intranasal and oral routes, causing 50%–100% mortality. The 35-d-old chickens suffered more severe infection than 7- and 21-d-old chickens with mortality highest in the intramuscular injection group. The serum from surviving chickens showed potent viral neutralizing capability. The complete genome of SD1511 was sequenced and analyzed. The strain was found to belong to the FAdV-4 cluster with more than 99% identity with the virulent FAdV-4 strains isolated in China in recent years except for some distinct variations, including deletions of open reading frame 27 (ORF27), ORF48, and part of ORF19. Our findings suggest that SD1511 might be used as a prototype strain for the study of pathogenesis and vaccine development.

禽腺病毒4型(FAdV-4)分离株对鸡的致病力及其基因组分析

目的:了解禽腺病毒4型(FAdV-4)分离株在口服、滴鼻点眼和肌注三种感染途径下对7~35日龄鸡的致病性,并分析该分离株与其他参考毒株在基因组水平上的差异.
创新点:用原代鸡胚肾细胞(CEK)成功培养了FAdV-4分离株.发现除了常用的口服和肌注途径,FAdV-4也可通过滴鼻点眼接种途径感染7、21和35日龄鸡.肌注方法最为敏感,导致感染鸡100%死亡;35日龄鸡较7和21日龄鸡表现出更明显的临床症状和剖检病变.
方法:通过口服、滴鼻点眼和肌注三种途径将FAdV-4分离株的CEK细胞培养物接种7~35日龄的无特定病原体(SPF)鸡.通过临床症状观察、排毒检测、病理解剖、免疫组化和组织中毒载量测定来判断分离株的致病性及病毒在组织脏器中的分布情况.通过病毒中和试验检测感染存活鸡的血清中和抗体.通过全基因组测序分析,比较FAdV-4分离株与国内外参考毒株的基因组序列差异.
结论:本研究分离鉴定的FAdV-4分离株SD1511可以在CEK细胞上适应,并可以通过口服、滴鼻点眼和肌注三种感染途径引起7~35日龄的SPF鸡50%~100%的死亡,其中肌注最为敏感,死亡率达100%.感染鸡持续排毒至感染后的40天,产生具有明显中和作用的中和抗体.全基因组测序分析结果显示,SD1511基因组与最近国内分离的FAdV-4具有很高的同源性,但与国外的分离株有明显的差异.

关键词:禽腺病毒4型(FAdV-4)分离株;致病性;感染途径;中和活性;基因组测序分析

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Alexander HS, Huber P, Cao J, et al., 1998. Growth characteristics of fowl adenovirus type 8 in a chicken hepatoma cell line. J Virol Methods, 74(1):9-14.

[2]Bose RTD, Grumbles LC, 1959. The relationship between quail bronchitis virus and chicken embryo lethal orphan virus. Avian Dis, 3(3):321-344.

[3]Choi KS, Kye SJ, Kim JY, et al., 2012. Epidemiological investigation of outbreaks of fowl adenovirus infection in commercial chickens in Korea. Poult Sci, 91(10):2502-2506.

[4]Chomiak TW, Luginbuhl RE, Helmboldt CF, 1961. Tissue culture propagation and pathology of celo virus. Avian Dis, 5(3):313-320.

[5]Ganesh K, Raghavan R, 2000. Hydropericardium hepatitis syndrome of broiler poultry: current status of research. Res Vet Sci, 68(3):201-206.

[6]Grgić H, Yang DH, Nagy É, 2011. Pathogenicity and complete genome sequence of a fowl adenovirus serotype 8 isolate. Virus Res, 156(1-2):91-97.

[7]Hess M, 2000. Detection and differentiation of avian adenoviruses: a review. Avian Pathol, 29(3):195-206.

[8]Jaffery MS, 1988. A treatise on angara disease (hydropericardium-pulmonary oedema-hepatonephritis syndrome). J Pak Vet Med Assoc, 34:1-33.

[9]Kaján GL, Kecskeméti S, Harrach B, et al., 2013. Molecular typing of fowl adenoviruses, isolated in hungary recently, reveals high diversity. Vet Microbiol, 167(3-4):357-363.

[10]Li HX, Wang J, Qiu LY, et al., 2016. Fowl adenovirus species C serotype 4 is attributed to the emergence of hepatitis-hydropericardium syndrome in chickens in China. Infect Genet Evol, 45:230-241.

[11]Li HY, Yin YB, Guo YY, et al., 2010. Isolation and PCR identification of 12 strains of inclusion body hepatitis virus from clinical broilers. Vet Sci China, 40(7):722-727 (in Chinese).

[12]Li PH, Zheng PP, Zhang TF, et al., 2017. Fowl adenovirus serotype 4: epidemiology, pathogenesis, diagnostic detection, and vaccine strategies. Poult Sci, 96(8):2630-2640.

[13]Liu YK, Wan WY, Gao DS, et al., 2016. Genetic characterization of novel fowl aviadenovirus 4 isolates from outbreaks of hepatitis-hydropericardium syndrome in broiler chickens in China. Emerg Microbes Infect, 5(1):1-8.

[14]Lund S, Holman GD, Schmitz O, et al., 1995. Contraction stimulates translocation of glucose transporter GLUT4 in skeletal muscle through a mechanism distinct from that of insulin. Proc Natl Acad Sci USA, 92(13):5817-5821.

[15]Mase M, Nakamura K, Minami F, 2012. Fowl adenoviruses isolated from chickens with inclusion body hepatitis in Japan, 2009–2010. J Vet Med Sci, 74(8):1087-1089.

[16]Mazaheri A, Prusas C, Voß M, et al., 1998. Some strains of serotype 4 fowl adenoviruses cause inclusion body hepatitis and hydropericardium syndrome in chickens. Avian Pathol, 27(3):269-276.

[17]Mittal D, Jindal N, Tiwari AK, et al., 2014. Characterization of fowl adenoviruses associated with hydropericardium syndrome and inclusion body hepatitis in broiler chickens. VirusDisease, 25(1):114-119.

[18]Ojkic D, Martin E, Swinton J, et al., 2008. Genotyping of Canadian isolates of fowl adenoviruses. Avian Pathol, 37(1):95-100.

[19]Park HS, Lim IS, Kim SK, et al., 2017. Molecular analysis of the hexon, penton base, and fiber-2 genes of Korean fowl adenovirus serotype 4 isolates from hydropericardium syndrome-affected chickens. Virus Genes, 53(1):111-116.

[20]Schachner A, Matos M, Grafl B, et al., 2018. Fowl adenovirus-induced diseases and strategies for their control—a review on the current global situation. Avian Pathol, 47(2):111-126.

[21]Schat K, Purchase HG, 1989. A Laboratory Manual for the Isolation and Identification of Avian Pathogens, 3rd Ed. American Association of Avian Pathologists, Kennett Square, PA, American, p.176-181.

[22]Ye JQ, Liang GC, Zhang JJ, et al., 2016. Outbreaks of serotype 4 fowl adenovirus with novel genotype, China. Emerg Microbes Infect, 5(1):1-12.

[23]Zhao J, Zhong Q, Zhao Y, et al., 2015. Pathogenicity and complete genome characterization of fowl adenoviruses isolated from chickens associated with inclusion body hepatitis and hydropericardium syndrome in China. PLoS ONE, 10(7):e0133073.

[24]List of electronic supplementary materials

[25]Table S1 Primers used to amplify the complete genomic sequence of FAdV-4 strain SD1511

[26]Table S2 Genome sequence identities (%) among SD1511 and FAdV-4 reference strains

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