CLC number: S858.31
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2019-07-09
Cited: 0
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Kai-Kun Mo, Chen-Fei Lyu, Shang-Shang Cao, Xia Li, Gang Xing, Yan Yan, Xiao-Juan Zheng, Min Liao, Ji-Yong Zhou. Pathogenicity of an FAdV-4 isolate to chickens and its genomic analysis[J]. Journal of Zhejiang University Science B, 2019, 20(9): 740-752.
@article{title="Pathogenicity of an FAdV-4 isolate to chickens and its genomic analysis",
author="Kai-Kun Mo, Chen-Fei Lyu, Shang-Shang Cao, Xia Li, Gang Xing, Yan Yan, Xiao-Juan Zheng, Min Liao, Ji-Yong Zhou",
journal="Journal of Zhejiang University Science B",
volume="20",
number="9",
pages="740-752",
year="2019",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1900070"
}
%0 Journal Article
%T Pathogenicity of an FAdV-4 isolate to chickens and its genomic analysis
%A Kai-Kun Mo
%A Chen-Fei Lyu
%A Shang-Shang Cao
%A Xia Li
%A Gang Xing
%A Yan Yan
%A Xiao-Juan Zheng
%A Min Liao
%A Ji-Yong Zhou
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 9
%P 740-752
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900070
TY - JOUR
T1 - Pathogenicity of an FAdV-4 isolate to chickens and its genomic analysis
A1 - Kai-Kun Mo
A1 - Chen-Fei Lyu
A1 - Shang-Shang Cao
A1 - Xia Li
A1 - Gang Xing
A1 - Yan Yan
A1 - Xiao-Juan Zheng
A1 - Min Liao
A1 - Ji-Yong Zhou
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 9
SP - 740
EP - 752
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1900070
Abstract: fowl adenovirus serotype 4 (FAdV-4) strain SD1511 was isolated from chickens with severe inclusion body hepatitis and hydropericardium syndrome in Shandong Province, China. The isolate was cultured in primary chicken embryo kidney cells. A study of pathogenicity indicated that SD1511 readily infected 7–35-d-old chickens by intramuscular injection and intranasal and oral routes, causing 50%–100% mortality. The 35-d-old chickens suffered more severe infection than 7- and 21-d-old chickens with mortality highest in the intramuscular injection group. The serum from surviving chickens showed potent viral neutralizing capability. The complete genome of SD1511 was sequenced and analyzed. The strain was found to belong to the FAdV-4 cluster with more than 99% identity with the virulent FAdV-4 strains isolated in China in recent years except for some distinct variations, including deletions of open reading frame 27 (ORF27), ORF48, and part of ORF19. Our findings suggest that SD1511 might be used as a prototype strain for the study of pathogenesis and vaccine development.
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[24]List of electronic supplementary materials
[25]Table S1 Primers used to amplify the complete genomic sequence of FAdV-4 strain SD1511
[26]Table S2 Genome sequence identities (%) among SD1511 and FAdV-4 reference strains
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