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Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.12 P.961-976


Detection of human papillomavirus in oropharyngeal squamous cell carcinoma

Author(s):  Fatin Hazwani Fauzi, Nurul Izzati Hamzan, Nurhayu Ab Rahman, Siti Suraiya, Suharni Mohamad

Affiliation(s):  School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia; more

Corresponding email(s):   suharni@usm.my

Key Words:  Human papillomavirus (HPV), Molecular detection, Oropharyngeal squamous cell carcinoma (OPSCC)

Fatin Hazwani Fauzi, Nurul Izzati Hamzan, Nurhayu Ab Rahman, Siti Suraiya, Suharni Mohamad. Detection of human papillomavirus in oropharyngeal squamous cell carcinoma[J]. Journal of Zhejiang University Science B, 2020, 21(12): 961-976.

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author="Fatin Hazwani Fauzi, Nurul Izzati Hamzan, Nurhayu Ab Rahman, Siti Suraiya, Suharni Mohamad",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Detection of human papillomavirus in oropharyngeal squamous cell carcinoma
%A Fatin Hazwani Fauzi
%A Nurul Izzati Hamzan
%A Nurhayu Ab Rahman
%A Siti Suraiya
%A Suharni Mohamad
%J Journal of Zhejiang University SCIENCE B
%V 21
%N 12
%P 961-976
%@ 1673-1581
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000161

T1 - Detection of human papillomavirus in oropharyngeal squamous cell carcinoma
A1 - Fatin Hazwani Fauzi
A1 - Nurul Izzati Hamzan
A1 - Nurhayu Ab Rahman
A1 - Siti Suraiya
A1 - Suharni Mohamad
J0 - Journal of Zhejiang University Science B
VL - 21
IS - 12
SP - 961
EP - 976
%@ 1673-1581
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000161

Worldwide there has been a significant increase in the incidence of oropharyngeal squamous cell carcinoma (OPSCC) etiologically attributed to oncogenic human papillomavirus (HPV). Reliable and accurate identification and detection tools are important as the incidence of HPV-related cancer is on the rise. Several HPV detection methods for OPSCC have been developed and each has its own advantages and disadvantages in regard to sensitivity, specificity, and technical difficulty. This review summarizes our current knowledge of molecular methods for detecting HPV in OPSCC, including HPV DNA/RNA polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), p16 immunohistochemistry (IHC), and DNA/RNA in situ hybridization (ISH) assays. This summary may facilitate the selection of a suitable method for detecting HPV infection, and therefore may help in the early diagnosis of HPV-related carcinoma to reduce its mortality, incidence, and morbidity.


概要:在世界范围内,人乳头瘤病毒(HPV)的致癌性导致口咽鳞状细胞癌(OPSCC)的发病率显著增加.随着HPV相关癌症发病率的上升,寻找可靠且准确的识别和检测HPV的工具变得非常重要.目前,虽然在OPSCC组织中检测HPV的方法已有多种,但是在灵敏度、特异性和技术难度等方面各有优缺点.本文综述了近年来应用于OPSCC组织中检测HPV的方法,包括HPV DNA/RNA聚合酶链反应(PCR)、环介导等温扩增(LAMP)、肿瘤蛋白16(p16)免疫组化(IHC)和DNA/RNA原位杂交分析(ISH),对选择合适的HPV检测方法具有指导意义,从而有助于HPV相关癌症的早期诊断,降低其死亡率和发病率.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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