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Bio-Design and Manufacturing  2023 Vol.6 No.3 P.298-318

http://doi.org/10.1007/s42242-022-00210-6


Designs and methodologies to recreate in vitro human gut microbiota models


Author(s):  Francesco Biagini, Costanza Daddi, Marco Calvigioni, Carmelo De Maria, Yu Shrike Zhang, Emilia Ghelardi & Giovanni Vozzi

Affiliation(s):  Research Center (E. Piaggio), University of Pisa, Pisa, Italy; more

Corresponding email(s):   g.vozzi@ing.unipi.it

Key Words:  Gut microbiota, In vitro models, Bioreactors, Host-microbiome, Fermentation


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Francesco Biagini, Costanza Daddi, Marco Calvigioni, Carmelo De Maria, Yu Shrike Zhang, Emilia Ghelardi & Giovanni Vozzi. Designs and methodologies to recreate in vitro human gut microbiota models[J]. Journal of Zhejiang University Science D, 2023, 6(3): 298-318.

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Abstract: 
The human gut microbiota is widely considered to be a metabolic organ hidden within our bodies, playing a crucial role in the hosts physiology. Several factors affect its composition, so a wide variety of microbes residing in the gut are present in the world population. Individual excessive imbalances in microbial composition are often associated with human disorders and pathologies, and new investigative strategies to gain insight into these pathologies and define pharmaceutical therapies for their treatment are needed. in vitro models of the human gut microbiota are commonly used to study microbial fermentation patterns, community composition, and host-microbe interactions. bioreactors and microfluidic devices have been designed to culture microorganisms from the human gut microbiota in a dynamic environment in the presence or absence of eukaryotic cells to interact with. In this review, we will describe the overall elements required to create a functioning, reproducible, and accurate in vitro culture of the human gut microbiota. In addition, we will analyze some of the devices currently used to study fermentation processes and relationships between the human gut microbiota and host eukaryotic cells.

比萨大学Giovanni Vozzi等 | 重建体外人类肠道微生物群模型的设计和方法

本综述论文聚焦人体肠道菌群体外重构中的设计思路实现方法。人类肠道微生物群被广泛认为是隐藏在我们体内的一个代谢器官,在宿主的生理学中起着至关重要的作用。有几个因素会影响其组成,因此,世界人口中存在着多种肠道微生物。人体微生物组成的过度失衡通常与人类疾病和病理有关,需要新的研究策略来深入了解这些病理并确定药物治疗方法。人体肠道微生物群的体外模型通常用于研究微生物发酵模式、群落组成和宿主微生物相互作用。生物反应器和微流体装置已被设计为在有/无与之相互作用的真核细胞的动态环境中培养来自人类肠道微生物群的微生物。在这篇综述中,我们将全面描述创建人体肠道微生物群的功能以及可复制和准确的体外培养所需的因素。此外,我们将分析目前用于研究发酵过程以及人体肠道微生物群与宿主真核细胞之间关系的一些设备。

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