Full Text:   <1998>

Summary:  <1701>

CLC number: 

On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

Crosschecked: 0000-00-00

Cited: 0

Clicked: 3588

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Hui CHEN

https://orcid.org/0000-0001-9185-1764

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.8 P.682-694

http://doi.org/10.1631/jzus.B2000697


Hemin-induced increase in saponin content contributes to the alleviation of osmotic and cold stress damage to Conyza blinii in a heme oxygenase 1-dependent manner


Author(s):  Tianrun ZHENG, Junyi ZHAN, Ming YANG, Maojia WANG, Wenjun SUN, Zhi SHAN, Hui CHEN

Affiliation(s):  College of Life Science, Sichuan Agricultural University, Ya’an 625014, China; more

Corresponding email(s):   chenhui@sicau.edu.cn

Key Words:  Hemin, Saponin, Conyza blinii, Heme oxygenase, Abiotic stress


Tianrun ZHENG, Junyi ZHAN, Ming YANG, Maojia WANG, Wenjun SUN, Zhi SHAN, Hui CHEN. Hemin-induced increase in saponin content contributes to the alleviation of osmotic and cold stress damage to Conyza blinii in a heme oxygenase 1-dependent manner[J]. Journal of Zhejiang University Science B, 2021, 22(8): 682-694.

@article{title="Hemin-induced increase in saponin content contributes to the alleviation of osmotic and cold stress damage to Conyza blinii in a heme oxygenase 1-dependent manner",
author="Tianrun ZHENG, Junyi ZHAN, Ming YANG, Maojia WANG, Wenjun SUN, Zhi SHAN, Hui CHEN",
journal="Journal of Zhejiang University Science B",
volume="22",
number="8",
pages="682-694",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2000697"
}

%0 Journal Article
%T Hemin-induced increase in saponin content contributes to the alleviation of osmotic and cold stress damage to Conyza blinii in a heme oxygenase 1-dependent manner
%A Tianrun ZHENG
%A Junyi ZHAN
%A Ming YANG
%A Maojia WANG
%A Wenjun SUN
%A Zhi SHAN
%A Hui CHEN
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 8
%P 682-694
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000697

TY - JOUR
T1 - Hemin-induced increase in saponin content contributes to the alleviation of osmotic and cold stress damage to Conyza blinii in a heme oxygenase 1-dependent manner
A1 - Tianrun ZHENG
A1 - Junyi ZHAN
A1 - Ming YANG
A1 - Maojia WANG
A1 - Wenjun SUN
A1 - Zhi SHAN
A1 - Hui CHEN
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 8
SP - 682
EP - 694
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000697


Abstract: 
hemin can improve the stress resistance of plants through the heme oxygenase system. Additionally, substances contained in plants, such as secondary metabolites, can improve stress resistance. However, few studies have explored the effects of hemin on secondary metabolite content. Therefore, the effects of hemin on saponin synthesis and the mechanism of plant injury relief by hemin in Conyza blinii were investigated in this study. hemin treatment promoted plant growth and increased the antioxidant enzyme activity and saponin content of C. blinii under osmotic stress and cold stress. Further study showed that hemin could provide sufficient precursors for saponin synthesis by improving the photosynthetic capacity of C. blinii and increasing the gene expression of key enzymes in the saponin synthesis pathway, thus increasing the saponin content. Moreover, the promotion effect of hemin on saponin synthesis is dependent on heme oxygenase-1 and can be reversed by the inhibitor Zn-protoporphyrin-IX (ZnPPIX). This study revealed that hemin can increase the saponin content of C. blinii and alleviate the damage caused by abiotic stress, and it also broadened the understanding of the relationship between hemin and secondary metabolites in plant abiotic stress relief.

氯化血红素通过血红素加氧酶-1依赖方式诱导皂甙含量增加,有助于减轻渗透和冷胁迫对金龙胆草的损伤

目的:研究氯化血红素对金龙胆草皂苷含量的影响,以及与植物非生物胁迫的相关性。
创新点:通过施加外源氯化血红素研究金龙胆草中皂苷的变化与冷胁迫或渗透胁迫的相关性。
方法:在渗透胁迫和低温胁迫下,施加外源氯化血红素及其抑制剂,检测并分析金龙胆草多项生理指标、皂苷含量差异和关键基因表达量差异。
结论:氯化血红素能通过依赖血红素加氧酶-1的调控方式,提高金龙胆草皂苷的含量,从而减轻非生物胁迫对金龙胆草的伤害。

关键词:氯化血红素;皂苷;金龙胆草;血红素加氧酶;非生物胁迫

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]BaconCW, PalenciaER, HintonDM, 2015. Abiotic and biotic plant stress-tolerant and beneficial secondary metabolites produced by endophytic Bacillus species. In: Arora NK (Ed.), Plant Microbes Symbiosis: Applied Facets. Springer, >New Delhi,p.163-177.

[2]BaudouinE, FrendoP, le GleuherM, et al., 2004. A Medicago sativa haem oxygenase gene is preferentially expressed in root nodules. J Exp Bot, 55(394):43-47.

[3]BeckerEM, CardosoDR, SkibstedLH, 2011. Quenching of excited states of red-pigment zinc protoporphyrin IX by hemin and natural reductors in dry-cured hams. Eur Food Res Technol, 232(2):343-349.

[4]ChenH, ChengZJ, MaXD, et al., 2013. A knockdown mutation of YELLOW-GREEN LEAF2 blocks chlorophyll biosynthesis in rice. Plant Cell Rep, 32(12):1855-1867.

[5]ChenQ, GongCY, JuX, et al., 2018. Hemin through the heme oxygenase 1/ferrous iron, carbon monoxide system involved in zinc tolerance in Oryza sativa L. J Plant Growth Regul, 37(3):947-957.

[6]DenneryPA, VisnerG, WengYH, et al., 2003. Resistance to hyperoxia with heme oxygenase-1 disruption: role of iron. Free Radic Biol Med, 34(1):124-133.

[7]EkhtariS, RazeghiJ, HasanpurK, et al., 2019. Different regulations of cell-type transcription by UV-B in multicellular green alga Volvox carteri. Plant Signal Behav, 14(11):1657339.

[8]FuGQ, ZhangLF, CuiWT, et al., 2011. Induction of heme oxygenase-1 with β-CD-hemin complex mitigates cadmium-induced oxidative damage in the roots of Medicago sativa. Plant Soil, 345(1-2):271-285.

[9]GengDS, ZhaoLP, ShiRM, et al., 2013. A study on antitussive, expectorant and anti-inflammatory effects of total saponins from Uyghur Herb Nigella glandulifera Freyn. J Xinjiang Med Univ, 36(7):908-911 (in Chinese).

[10]GhasemzadehA, JaafarHZE, 2011. Effect of CO2 enrichment on synthesis of some primary and secondary metabolites in ginger (Zingiber officinale Roscoe). Int J Mol Sci, 12(2):1101-1114.

[11]GheisariHR, MøllerJKS, AdamsenCE, et al., 2010. Sodium chloride or heme protein induced lipid oxidation in raw, minced chicken meat and beef. Czech J Food Sci, 28(5):364-375.

[12]GitelsonAA, GritzY, MerzlyakMN, 2003. Relationships between leaf chlorophyll content and spectral reflectance and algorithms for non-destructive chlorophyll assessment in higher plant leaves. J Plant Physiol, 160(3):271-282.

[13]IshikawaK, SugawaraD, WangXP, et al., 2001. Heme oxygenase-1 inhibits atherosclerotic lesion formation in LDL-receptor knockout mice. Circ Res, 88(5):506-512.

[14]JiangY, SunFF, ZhanYG, et al., 2019. Effect of carbon monoxide on growth and triterpenoid production in Betula platyphylla suspension cells. Chin Tradit Herb Drugs, 50(15):3681-3686 (in Chinese).

[15]JinQJ, ZhuKK, XieYJ, et al., 2013. Heme oxygenase-1 is involved in ascorbic acid-induced alleviation of cadmium toxicity in root tissues of Medicago sativa. Plant Soil, 366(1-2):605-616.

[16]JinQJ, CuiWT, DaiC, et al., 2016. Involvement of hydrogen peroxide and heme oxygenase-1 in hydrogen gas-induced osmotic stress tolerance in alfalfa. Plant Growth Regul, 80(2):215-223.

[17]JungHY, KimDW, YimHS, et al., 2016. Heme oxygenase-1 protects neurons from ischemic damage by upregulating expression of Cu,Zn-superoxide dismutase, catalase, and brain-derived neurotrophic factor in the rabbit spinal cord. Neurochem Res, 41(4):869-879.

[18]LiH, JiangM, CheLL, et al., 2012. BjHO-1 is involved in the detoxification of heavy metal in India mustard (Brassica juncea). BioMetals, 25(6):1269-1279.

[19]LiuHY, HuCX, SunNN, et al., 2017. A triterpenoidal saponin fraction of Conyza blinii H.‍Lév. is a dual-targeting autophagy inhibitor for HeLa cells. RSC Adv, 7(39):24291-24297.

[20]LiuP, ZhouLJ, SuYF, et al., 2011. Effects of total saponins from Conyza blinii on the apoptosis of Hela cells and SPC-A1 cells. China Pharmacy, 22(35):3288-3291 (in Chinese).

[21]LiuYY, ChenQ, LiN, et al., 2016. Heme oxygenase-1 (HO-1) promote anthocyanin accumulation in the hypocotyl of radish sprouts. Acta Horticult Sin, 43(3):507-514 (in Chinese).

[22]LuoWW, WangY, YangHW, et al., 2018. Heme oxygenase-1 ameliorates oxidative stress-induced endothelial senescence via regulating endothelial nitric oxide synthase activation and coupling. Aging, 10(7):1722-1744.

[23]MaL, LiuJG, 2014. The protective activity of Conyza blinii saponin against acute gastric ulcer induced by ethanol. J Ethnopharmacol, 158:358-363.

[24]MaL, LiuHY, MengLP, et al., 2017a. Evaluation of the anti-cancer activity of the triterpenoidal saponin fraction isolated from the traditional Chinese medicine Conyza blinii H. Lév. RSC Adv, 7(6):3408-3412.

[25]MaL, LiuHY, QinP, et al., 2017b. Saponin fraction isolated from Conyza blinii H.Lév. demonstrates strong anti-cancer activity that is due to its NF-κB inhibition. Biochem Biophys Res Commun, 483(1):779-785.

[26]MainesMD, PanahianN, 2001. The heme oxygenase system and cellular defense mechanisms. Do HO-1 and HO-2 have different functions? In: Roach RC, Wagner PD, Hackett PH (Eds.), Hypoxia: From Genes to the Bedside. Springer, Boston, p.249-272.

[27]MatthusE, WilkinsKA, SwarbreckSM, et al., 2019. Phosphate starvation alters abiotic-stress-induced cytosolic free calcium increases in roots. Plant Physiol, 179(4):1754-1767.

[28]MeiYQ, SongSQ, 2010. Response to temperature stress of reactive oxygen species scavenging enzymes in the cross-tolerance of barley seed germination. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 11(12):965-972.

[29]MüllebnerA, MoldzioR, RedlH, et al., 2015. Heme degradation by heme oxygenase protects mitochondria but induces ER stress via formed bilirubin. Biomolecules, 5(2):679-701.

[30]NoriegaG, CruzDS, BatlleA, et al., 2012. Heme oxygenase is involved in the protection exerted by jasmonic acid against cadmium stress in soybean roots. J Plant Growth Regul, 31(1):79-89.

[31]NoriegaGO, BalestrasseKB, BatlleA, et al., 2004. Heme oxygenase exerts a protective role against oxidative stress in soybean leaves. Biochem Biophys Res Commun, 323(3):1003-1008.

[32]NzowaLK, BarboniL, TeponnoRB, et al., 2010. Rheediinosides A and B, two antiproliferative and antioxidant triterpene saponins from Entada rheedii. Phytochemistry, 71(2-3):254-261.

[33]OrreniusS, KassGEN, NicoteraP, 1992. Oxygen free radicals and cell death. Neurochem Int, 21(Suppl):S13.

[34]OtterbeinLE, SoaresMP, YamashitaK, et al., 2003. Heme oxygenase-1: unleashing the protective properties of heme. Trends Immunol, 24(8):449-455.

[35]OzdenerY, AydinBK, 2010. The effect of zinc on the growth and physiological and biochemical parameters in seedlings of Eruca sativa (L.) (Rocket). Acta Physiol Plant, 32(3):469-476.

[36]PodkalickaP, MuchaO, JózkowiczA, et al., 2018. Heme oxygenase inhibition in cancers: possible tools and targets. Contemp Oncol, 2(1A):23-32.

[37]ShahK, NahakpamS, 2012. Heat exposure alters the expression of SOD, POD, APX and CAT isozymes and mitigates low cadmium toxicity in seedlings of sensitive and tolerant rice cultivars. Plant Physiol Biochem, 57:106-113.

[38]ShamloulR, WangR, 2006. Increased intracavernosal pressure response in hypertensive rats after chronic hemin treatment. J Sex Med, 3(4):619-627.

[39]SuYF, KoikeK, GuoDA, et al., 2001. New apiose-containing triterpenoid saponins from Conyza blinii. Tetrahedron, 57(31):6721-6726.

[40]SunR, LiuS, GaoJL, et al., 2014. Cloning and expression analysis of 1-deoxy-D-xylulose-5-phosphate synthase gene from the medicinal plant Conyza blinii H.Lév. Turk J Biol, 38(5):664-670.

[41]SunWJ, ZhanJY, ZhengTR, et al., 2018. The jasmonate-responsive transcription factor CbWRKY24 regulates terpenoid biosynthetic genes to promote saponin biosynthesis in Conyza blinii H. Lév. J Genet, 97(5):1379-1388.

[42]TroxlerRF, 1972. Synthesis of bile pigments in plants.Formation of carbon monoxide and phycocyanobilin in wild-type and mutant strains of the alga, Cyanidium caldarium. Biochemistry, 11(23):4235-4242.

[43]VeliniED, TrindadeMLB, AlvesE, et al., 2005. Eucalyptus ESTs corresponding to the protoporphyrinogen IX oxidase enzyme related to the synthesis of heme, chlorophyll, and to the action of herbicides. Genet Mol Biol, 28(3 Suppl):548-554.

[44]WagenerFADTG, VolkHD, WillisD, et al., 2003. Different faces of the heme-heme oxygenase system in inflammation. Pharmacol Rev, 55(3):551-571.

[45]WangJ, DoréS, 2007. Heme oxygenase-1 exacerbates early brain injury after intracerebral haemorrhage. Brain, 130(6):1643-1652.

[46]WangSQ, WangXM, SunFF, et al., 2019. Carbon monoxide: effect on growth and triterpene accumulation of phellinus linteus mycelium. Chin Agric Sci Bull, 35(21):90-95 (in Chinese).

[47]XiongCW, LiXW, XuDJ, et al., 2017. Determination of total saponins in quinoa by spectrophotometry. J Anhui Agric Sci, 45(26):96-98,121 (in Chinese).

[48]XuanW, ZhuFY, XuS, et al., 2008. The heme oxygenase/carbon monoxide system is involved in the auxin-induced cucumber adventitious rooting process. Plant Physiol, 148(2):881-893.

[49]YangCR, HeZT, LiXC, et al., 1989. Blinin, a neoclerodane diterpene from Conyza blinii. Phytochemistry, 28(11):3131-3134.

[50]YannarelliGG, NoriegaGO, BatlleA, et al., 2006. Heme oxygenase up-regulation in ultraviolet-B irradiated soybean plants involves reactive oxygen species. Planta, 224(5):1154-1162.

[51]ZhanJY, YangQ, LinZY, et al., 2021. Enhanced antioxidant capacity and upregulated transporter genes contribute to the UV-B-induced increase in blinin in Conyza blinii. Environ Sci Pollut Res, 28(11):13275-13287.

[52]ZhengTR, WangMJ, ZhanJY, et al., 2020. Ferrous iron-induced increases in capitate glandular trichome density and upregulation of CbHO-1 contributes to increases in blinin content in Conyza blinii. Planta, 252(5):81.

[53]ZhuZB, HuangYF, WuX, et al., 2019. Increased antioxidative capacity and decreased cadmium uptake contribute to hemin-induced alleviation of cadmium toxicity in Chinese cabbage seedlings. Ecotoxicol Environ Saf, 177:47-57.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE