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Received: 2003-09-22

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Bio-Design and Manufacturing  2022 Vol.5 No.6 P.733~737

10.1631/jzus.2004.0733


Human cytomegalovirus induces alteration of β-actin mRNA and microfilaments in human embryo fibroblast cells


Author(s):  LIN Mao-fang, WEI Guo-qing, HUANG He, CAI Zhen

Affiliation(s):  Department of Hematology, First Affiliated Hospital, College of Medical Science, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   weiguoqing2000@hotmail.com

Key Words:  Cytomegalovirus, Human embryo fibroblast cells, Infection, &beta, -actin, Microfilament


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LIN Mao-fang, WEI Guo-qing, HUANG He, CAI Zhen. Human cytomegalovirus induces alteration of β-actin mRNA and microfilaments in human embryo fibroblast cells[J]. Journal of Zhejiang University Science D, 2022, 5(6): 733~737.

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author="LIN Mao-fang, WEI Guo-qing, HUANG He, CAI Zhen",
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volume="5",
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year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.0733"
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%T Human cytomegalovirus induces alteration of β-actin mRNA and microfilaments in human embryo fibroblast cells
%A LIN Mao-fang
%A WEI Guo-qing
%A HUANG He
%A CAI Zhen
%J Journal of Zhejiang University SCIENCE D
%V 5
%N 6
%P 733~737
%@ 1869-1951
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.0733

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T1 - Human cytomegalovirus induces alteration of β-actin mRNA and microfilaments in human embryo fibroblast cells
A1 - LIN Mao-fang
A1 - WEI Guo-qing
A1 - HUANG He
A1 - CAI Zhen
J0 - Journal of Zhejiang University Science D
VL - 5
IS - 6
SP - 733
EP - 737
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2004.0733


Abstract: 
Objective: To investigate the infection of human embryo fibroblast cell line HF cells by CMV as well as the effects of CMV on &beta;-actin mRNA and microfilaments. Methods: HF cells shape was observed after the infection of CMV. RT-PCR assay was used to detect the mRNA expression of CMV immediate early (IE) gene, &beta;-actin and GAPDH genes of HF cells infected by CMV. CMV particles and cell microfilaments were detected with electron microscope. Results: Shape of HF cell changed after the infection by CMV. HF cells infected by CMV could express IE mRNA and the expression of &beta;-actin mRNA decreased in a time- and titer-dependent manner compared with the uninfected HF cells whose expression of GAPDH mRNA did not change much. CMV particles were found with electron microscope in the cells. microfilaments were ruptured and shortened after the infection of CMV. Conclusion: CMV can not only infect human embryo fibroblast cells line HF cells and replicate in the cells, but can also affect the expression of &beta;-actin mRNA and the microfilaments.

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Reference

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[4] Gupta, S., De, B.P., Drazba, J.A., 1998. Involvement of actin microfilaments in the replication of human parainfluenza virus type 3.J Virol,72:2655-2662.

[5] Jones, N.L., Lewis, J.C., Kilpatrick, B.A., 1986. Cytoskeletal disruption during human cytomegalovirus infection of human lung fibroblasts.Eur J Cell Biol,41:304-312.

[6] Liotta, L.A., Steler, W.G., 1991. Tumor invasion and metastasis: an imbalance of positive and negative regulation.Cancer Res,51:5054.

[7] Ravkov, E.V., Nichol, S.T., Peters, C.J., 1998. Role of actin microfilaments in Black Creek Canal virus morphogenesis.J Virol,72:2865-2870.

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