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Bio-Design and Manufacturing  2018 Vol.5 No.10 P.1249~1254

10.1631/jzus.2004.1249


Identification of embryonic chromosomal abnormality using FISH-based preimplantaion genetic diagnosis


Author(s):  YE Ying-hui, XU Chen-ming, JIN Fan, QIAN Yu-li

Affiliation(s):  Department of Reproductive Endocrinology, Affiliated Women's Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China

Corresponding email(s):   yeyhcn2000@yahoo.com.cn

Key Words:  Preimplantation genetic diagnosis, Fluorescence in-situ Hybridization (FISH), Chromosome abnormality


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YE Ying-hui, XU Chen-ming, JIN Fan, QIAN Yu-li. Identification of embryonic chromosomal abnormality using FISH-based preimplantaion genetic diagnosis[J]. Journal of Zhejiang University Science D, 2018, 5(10): 1249~1254.

@article{title="Identification of embryonic chromosomal abnormality using FISH-based preimplantaion genetic diagnosis",
author="YE Ying-hui, XU Chen-ming, JIN Fan, QIAN Yu-li",
journal="Journal of Zhejiang University Science D",
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number="10",
pages="1249~1254",
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publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.1249"
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%T Identification of embryonic chromosomal abnormality using FISH-based preimplantaion genetic diagnosis
%A YE Ying-hui
%A XU Chen-ming
%A JIN Fan
%A QIAN Yu-li
%J Journal of Zhejiang University SCIENCE D
%V 5
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%P 1249~1254
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.1249

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T1 - Identification of embryonic chromosomal abnormality using FISH-based preimplantaion genetic diagnosis
A1 - YE Ying-hui
A1 - XU Chen-ming
A1 - JIN Fan
A1 - QIAN Yu-li
J0 - Journal of Zhejiang University Science D
VL - 5
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SP - 1249
EP - 1254
%@ 1869-1951
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2004.1249


Abstract: 
Objective: Embryonic chromosomal abnormality is one of the main reasons for in vitro fertilization (IVF) failure. This study aimed at evaluating the value of fluorescence in-situ Hybridization (FISH)-based preimplantation genetic diagnosis (PGD) in screening for embryonic chromosomal abnormality to increase the successful rate of IVF. Method: Ten couples, four with high risk of chromosomal abnormality and six infertile couples, underwent FISH-based PGD during IVF procedure. At day 3, one or two blastomeres were aspirated from each embryo. Biopsied blastomeres were examined using FISH analysis to screen out embryos with chromosomal abnormalities. At day 4, embryos without detectable chromosomal abnormality were transferred to the mother bodies as in regular IVF. Results: Among 54 embryos screened using FISH-based PGD, 30 embryos were detected to have chromosomal abnormalities. The 24 healthy embryos were implanted, resulting in four clinical pregnancies, two of which led to successful normal birth of two healthy babies; one to ongoing pregnancy during the writing of this article; and one to ectopic pregnancy. Conclusion: FISH-based PGD is an effective method for detecting embryonic chromosomal abnormality, which is one of the common causes of spontaneous miscarriages and chromosomally unbalanced offsprings.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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