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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.8 P.725~730


Biodegradation of crude oil by Pseudomonas aeruginosa in the presence of rhamnolipids

Author(s):  ZHANG Guo-liang, WU Yue-ting, QIAN Xin-ping, MENG Qin

Affiliation(s):  School of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   mengq@zju.edu.cn

Key Words:  Rhamnolipid, Crude oil, Biodegradation, Pseudomonas aeruginosa

ZHANG Guo-liang, WU Yue-ting, QIAN Xin-ping, MENG Qin. Biodegradation of crude oil by Pseudomonas aeruginosa in the presence of rhamnolipids[J]. Journal of Zhejiang University Science B, 2005, 6(8): 725~730.

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T1 - Biodegradation of crude oil by Pseudomonas aeruginosa in the presence of rhamnolipids
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The potential biodegradation of crude oil was assessed based on the development of a fermentative process with a strain of Pseudomonas aeruginosa which produced 15.4 g/L rhamnolipids when cultured in a basal mineral medium using glycerol as a sole carbon source. However, neither cell growth nor rhamnolipid production was observed in the comparative culture system using crude oil as the sole carbon source instead. As rhamnolipid, an effective biosurfactant, has been reported to stimulate the biodegradation of hydrocarbons, 1 g/L glycerol or 0.22 g/L rhamnolipid was initially added into the medium to facilitate the biodegradation of crude oil. In both situations, more than 58% of crude oil was degraded and further converted into accumulated cell biomass and rhamnolipids. These results suggest that Pseudomonas aeruginosa could degrade most of crude oil with direct or indirect addition of rhamnolipid. And this conclusion was further supported by another adsorption experiment, where the adsorption capacity of crude oil by killed cell biomass was negligible in comparison with the biologic activities of live cell biomass.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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farzana jaslina@UPM<safar_18994@yahoo.com>

2016-10-26 11:38:46

Hi. I'm doing my Final Year Project regarding Pseudomonas aeruginosa and i would like to refer to your article for my study. Thanks :)

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