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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.8 P.725~730

http://doi.org/10.1631/jzus.2005.B0725


Biodegradation of crude oil by Pseudomonas aeruginosa in the presence of rhamnolipids


Author(s):  ZHANG Guo-liang, WU Yue-ting, QIAN Xin-ping, MENG Qin

Affiliation(s):  School of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   mengq@zju.edu.cn

Key Words:  Rhamnolipid, Crude oil, Biodegradation, Pseudomonas aeruginosa


ZHANG Guo-liang, WU Yue-ting, QIAN Xin-ping, MENG Qin. Biodegradation of crude oil by Pseudomonas aeruginosa in the presence of rhamnolipids[J]. Journal of Zhejiang University Science B, 2005, 6(8): 725~730.

@article{title="Biodegradation of crude oil by Pseudomonas aeruginosa in the presence of rhamnolipids",
author="ZHANG Guo-liang, WU Yue-ting, QIAN Xin-ping, MENG Qin",
journal="Journal of Zhejiang University Science B",
volume="6",
number="8",
pages="725~730",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0725"
}

%0 Journal Article
%T Biodegradation of crude oil by Pseudomonas aeruginosa in the presence of rhamnolipids
%A ZHANG Guo-liang
%A WU Yue-ting
%A QIAN Xin-ping
%A MENG Qin
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 8
%P 725~730
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0725

TY - JOUR
T1 - Biodegradation of crude oil by Pseudomonas aeruginosa in the presence of rhamnolipids
A1 - ZHANG Guo-liang
A1 - WU Yue-ting
A1 - QIAN Xin-ping
A1 - MENG Qin
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 8
SP - 725
EP - 730
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0725


Abstract: 
The potential biodegradation of crude oil was assessed based on the development of a fermentative process with a strain of Pseudomonas aeruginosa which produced 15.4 g/L rhamnolipids when cultured in a basal mineral medium using glycerol as a sole carbon source. However, neither cell growth nor rhamnolipid production was observed in the comparative culture system using crude oil as the sole carbon source instead. As rhamnolipid, an effective biosurfactant, has been reported to stimulate the biodegradation of hydrocarbons, 1 g/L glycerol or 0.22 g/L rhamnolipid was initially added into the medium to facilitate the biodegradation of crude oil. In both situations, more than 58% of crude oil was degraded and further converted into accumulated cell biomass and rhamnolipids. These results suggest that Pseudomonas aeruginosa could degrade most of crude oil with direct or indirect addition of rhamnolipid. And this conclusion was further supported by another adsorption experiment, where the adsorption capacity of crude oil by killed cell biomass was negligible in comparison with the biologic activities of live cell biomass.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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[13] Yong, K.C., Ooi, T.L., Dzulkefly, K., Wanyunus, W.M.Z., Hazimah, A.H., 2001. Characterization of glycerol residue from a palm kernel oil methyl ester plant. J. of Oil Palm Research, 13(2):1-6.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

farzana jaslina@UPM<safar_18994@yahoo.com>

2016-10-26 11:38:46

Hi. I'm doing my Final Year Project regarding Pseudomonas aeruginosa and i would like to refer to your article for my study. Thanks :)

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