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Abstract: To overcome the pain and risk of hypoglycemia in insulin administration, glucose-responsive microneedles have been developed by researchers, which could release insulin according to the blood glucose level. We designed a kind of particles by a reversible addition-fragmentation chain transfer (RAFT) method containing a phenylboronic acid group as the sensor of glucose and carrier of insulin. poly(ethylene glycol) (PEG)‍-2-‍(dodecylthio(thiocarbonyl)thio)‍-2-methylpropionic acid (DDMAT) was synthesized as a macromolecular RAFT agent, which was then reacted with 3-acrylamidophenylboronic acid (AAPBA) to synthesize the block copolymer PEG-b-PAAPBA. Glucose-responsive particles loaded with insulin were prepared by self-assembly based on hydrophilic-hydrophobic interactions. Microneedle patches loaded with glucose-responsive particles were prepared using hyaluronic acid as the substrate. The insulin release behavior of the particles in glucose solutions of 0, 100, and 400 mg/dL showed significant glucose responsiveness and good biosafety. The results of blood glucose control experiments in rats indicate that a single microneedle patch can effectively maintain normal blood glucose for over 7 h.

聚乙二醇-b-聚丙烯酰胺基苯硼酸葡萄糖响应粒子及其透明质酸微针贴片的制备与性能研究

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