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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.7 P.520~526

http://doi.org/10.1631/jzus.B0710618


Induction of nucleoside phosphorylase in Enterobacter aerogenes and enzymatic synthesis of adenine arabinoside


Author(s):  Xiao-kun WEI, Qing-bao DING, Lu ZHANG, Yong-li GUO, Lin OU, Chang-lu WANG

Affiliation(s):  College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; more

Corresponding email(s):   weixiaokun99@163.com, qbding@ecust.edu.cn

Key Words:  Nucleoside phosphorylase (NPase), Enterobacter aerogenes, Cytidine, Cytidine 5&prime, -monophosphate (CMP), Adenine arabinoside


Xiao-kun WEI, Qing-bao DING, Lu ZHANG, Yong-li GUO, Lin OU, Chang-lu WANG. Induction of nucleoside phosphorylase in Enterobacter aerogenes and enzymatic synthesis of adenine arabinoside[J]. Journal of Zhejiang University Science B, 2008, 9(7): 520~526.

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author="Xiao-kun WEI, Qing-bao DING, Lu ZHANG, Yong-li GUO, Lin OU, Chang-lu WANG",
journal="Journal of Zhejiang University Science B",
volume="9",
number="7",
pages="520~526",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0710618"
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%T Induction of nucleoside phosphorylase in Enterobacter aerogenes and enzymatic synthesis of adenine arabinoside
%A Xiao-kun WEI
%A Qing-bao DING
%A Lu ZHANG
%A Yong-li GUO
%A Lin OU
%A Chang-lu WANG
%J Journal of Zhejiang University SCIENCE B
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%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0710618

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T1 - Induction of nucleoside phosphorylase in Enterobacter aerogenes and enzymatic synthesis of adenine arabinoside
A1 - Xiao-kun WEI
A1 - Qing-bao DING
A1 - Lu ZHANG
A1 - Yong-li GUO
A1 - Lin OU
A1 - Chang-lu WANG
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 7
SP - 520
EP - 526
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0710618


Abstract: 
Nucleoside phosphorylases (NPases) were found to be induced in Enterobacter aerogenes DGO-04, and cytidine and cytidine 5′-monophosphate (CMP) were the best inducers. Five mmol/L to fifteen mmol/L cytidine or CMP could distinctly increase the activities of purine nucleoside phosphorylase (PNPase), uridine phosphorylase (UPase) and thymidine phosphorylase (TPase) when they were added into medium from 0 to 8 h. In the process of enzymatic synthesis of adenine arabinoside from adenine and uracil arabinoside with wet cells of Enterobacter aerogenes DGO-04 induced by cytidine or CMP, the reaction time could be shortened from 36 to 6 h. After enzymatic reaction the activity of NPase in the cells induced remained higher than that in the cells uninduced.

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Reference

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