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Received: 2007-08-27

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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.2 P.141~147


Hypothetical mode of action of earthworm extract with hepatoprotective and antioxidant properties

Author(s):  Mariappan BALAMURUGAN, Kasi PARTHASARATHI, Lalpet Souri RANGANATHAN, Edwin L. COOPER

Affiliation(s):  Division of Vermibiotechnology, Department of Zoology, Annamalai University, Annamalai Nagar-608002, India; more

Corresponding email(s):   dnabala@rediffmail.com

Key Words:  Earthworm extract, Hepatoprotective potential, Antioxidant, Paracetamol, Lipid peroxidation

Mariappan BALAMURUGAN, Kasi PARTHASARATHI, Lalpet Souri RANGANATHAN, Edwin L. COOPER. Hypothetical mode of action of earthworm extract with hepatoprotective and antioxidant properties[J]. Journal of Zhejiang University Science B, 2008, 9(2): 141~147.

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publisher="Zhejiang University Press & Springer",

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%A Edwin L. COOPER
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0720194

T1 - Hypothetical mode of action of earthworm extract with hepatoprotective and antioxidant properties
A1 - Mariappan BALAMURUGAN
A1 - Lalpet Souri RANGANATHAN
A1 - Edwin L. COOPER
J0 - Journal of Zhejiang University Science B
VL - 9
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EP - 147
%@ 1673-1581
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B0720194

The hepatoprotective potential of earthworm extract (EE) (Lampito mauritii, Kinberg) was evaluated against paracetamol-induced liver injury in Wistar albino rat, in comparison with silymarin, the standard hepatoprotective drug. We observed a reduction in liver antioxidants, such as glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) and in serum total protein, and an increase in serum alkaline phosphatase (ALP), serum aspertate aminotranferase (AST), serum alanine aminotranferase (ALT), bilirubin and liver thiobarbituric acid reactive substances (TBARS) due to liver injury in the paracetamol-administered rats (2 g/kg). On the contrary, increased activities of liver GSH, SOD, GPx, CAT and serum total protein level, and decrease in the contents of serum ALP, AST, ALT, bilirubin and liver TBARS were observed in rats administered with different doses of EE (100, 200 and 300 mg/kg), which are similar to the activities of hepatoprotective drug silymarin (150 mg/kg). The mode of action of EE as evidenced by the above parameters may suggest that EE, on the one hand, prevents the formation of the reactive oxygen groups, or scavenges these groups, thereby preventing the damage on the hepatic cells, and, on the other hand, modulates the genes responsible for synthesis of antioxidant enzymes such as GPx, CAT and SOD in liver tissue and decreases the serum enzymatic activities such as ALP, AST and ALT.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Alum, J., Camhi, S., Choi, A.M., 1995. Identification of a second region upstream of the mouse heme oxygenase-1 gene that functions as a basal level and inducer dependent transcription enhancer. J. Biol. Chem., 270(20):11977-11984.

[2] Applegate, L.A., Luscher, P., Tyrrell, R.M., 1991. Induction of heme oxygenase: A general response to oxidant stress in cultured mammalian cells. Cancer Res., 51:974-978.

[3] Balamurugan, M., Parthasarathi, K., Cooper, E.L., Ranganathan, L.S., 2007. Earthworm paste (Lampito mauritii, Kinberg) alters inflammatory, oxidative, haematological and serum biochemical indices of inflamed rat. European Review for Medical and Pharmacological Sciences, 11(1):77-90.

[4] Bergmeyer, H.U., Scelibe, P., Wahlefeld, A.W., 1978. Optimization of methods for aspirate aminotransferase and alanine aminotransferase. Clin. Chem., 24:58-61.

[5] Bhar, M.K., Das, S.K., Charkraborty, A.K., Mandal, T.K., Roy, S., 2005. Hepatoprotective effect of Enliv on paracetamol-induced liver damage in broiler chicken. Indian J. Pharmacol., 37(4):257-258.

[6] Cooper, E.L., 2005. CAM, eCAM, bioprospecting: The 21st century pyramid. Evidence-based Complementary and Alternative Medicine, 2(2):125-127.

[7] Crawford, D.R., 1999. Regulation of Mammalian Gene Expression by Reactive Oxygen Spicies. In: Gilbert, D., Cotton, C. (Eds.), Reactive Oxygen Species in Biological System. Plenum, New York, p.155-171.

[8] Ellman, G.C., 1959. Tissue sulfhydryl groups. Arch. Biochem. Biophys., 82(1):70-77.

[9] Gupta, M., Mazumder, U.K., Sivakumar, T., Gomathi, P., Sampathkumar, R., 2004. Antioxidant and hepatoprotective effects of Bauhinia racemosa against paracetamol and carbon tetrachloride induced liver damage in rats. IJPT, 3:12-20.

[10] Halliwell, B., Gutteridge, J.M., 1984. Lipid peroxidation, oxygen radicals, cell damage, and antioxidant therapy. Lancet, 323(8391):1396-1397.

[11] Hrzenjak, T., Hrzenjak, M., Kasuba, V., Efenberger-Marinculic, P., Levanat, S., 1992. A new source of active compounds—earthworm tissue (Eisenia foetida, Lumbricus rubellus). Comp. Biochem. Physiol. Part A Physiol., 102(3):441-447.

[12] Ismail, S.A., Pulandiran, K., Yegnanarayan, R., 1992. Anti-inflammatory activity of earthworm extracts. Soil Biol. Biochem., 24(12):1253-1254.

[13] Jollow, D.J., 1980. Glutathione thresholds in reactive metabolite toxicity. Arch. Toxicol. Suppl., 3:95-110.

[14] Kakkar, P., Das, B., Viswanathan, P.N., 1984. A modified spectroscopic assay of superoxide dismutase. Indian J. Biochem. Biophys., 21:130-132.

[15] King, E.J., Armstrong, A.R., 1934. Determination of serum and bile phosphatase activity. J. Can. Med. Assoc., 31:376-379.

[16] Leeuwenburgh, C., Ji, L.L., 1995. Glutathione depletion in rested and exercised mice: Biochemical consequence and adaptation. Arch. Biochem. Biophys., 316(2):941-949.

[17] Lowry, O.H., Rose Brough, M.J., Farr, A.L., Randall, R.J., 1951. Protein measurement with Folin-phenol reagent. J. Biol. Chem., 193:265-275.

[18] Malloy, H.J., Evelyn, K.A., 1937. The determination of bilirubin with the photoelectric colorimeter. J. Biol. Chem., 119:481-490.

[19] Moss, D.W., Butterworth, P.J., 1974. Enzymology and Medicine. Pitman Medical, London, p.139.

[20] Muriel, P., Garciapina, T., Perez-Alvarez, V., Murelle, M., 1992. Silymarin protect against paracetamol-induced lipid peroxidation and liver damage. Drug Chem. Toxicol., 1:163-171.

[21] Niehans, W.G., Samuelsson, D., 1968. Formation of malondialdehyde from phospholipids arrachidonate during microsomal lipid peroxidation. Eur. J. Biochem., 6(1):126-130.

[22] Prakash, M., Balamurugan, M., Parthasarathi, K., Gunasekaran, G., Cooper, E.L., Ranganathan, L.S., 2007. Anti-ulceral and anti-oxidative properties of “earthworm paste” of Lampito mauritii (Kinberg) on Rattus Norvegicus. European Review for Medical and Pharmacological Sciences, 11(1):9-15.

[23] Ranganathan, L.S., 2006. Vermibiotechnology—from Soil Health to Human Health. Agrobios, Jodhpur, India.

[24] Rotruck, J.T., Rope, A.L., Ganther, H.F., Swason, A.B., 1973. Selenium: Biochemical role as a component of glutathione peroxide. Science, 179(4073):588-590.

[25] Sadasivan, S., Latha, P.G., Sasikumar, J.M., Rajashekaran, S., Shyamal, S., Shine, V.J., 2006. Hepatoprotective studies on Hedyotis corymbosa (L.) Lam. J. Ethnopharmacol., 106(2):245-249.

[26] Sallie, R., Tredgeri, J.M., Willion, R., 1991. Drugs and the liver. Biopharmaceutics and Drug Disposition, 12(4):251-259.

[27] Scott, L.N.D., 1998. A review of plants used in the treatment of liver disease: Part I. Altern. Med. Rev., 3(6):410-421.

[28] Sheila, S., Dooley, J., 1993. Diseases of the Liver and Biliary System, 9th Ed. Backwell Scientific Publications, Osney Mead, Oxford OX2 OEL, p.1-16.

[29] Shull, S., Heintz, N.H., Periasamy, M., Manohor, M., Jansseny, M., Marsh, J.P., Mossman, B.T., 1991. Differential regulation of antioxidant enzymes in response to oxidants. J. Biol. Chem., 266:24398-24403.

[30] Sinha, K.A., 1972. Colorimetric assay of catalase. Ann. Biochem., 47(2):389-394.

[31] Stocker, R., Yamamoto, Y., McDonagh, A.F., Glazer, A.N., Ames, B.N., 1987. Bilirubin is an antioxidant of possible physiologic importance. Science, 235(4792):1043-1046.

[32] Tabassum, N., Chattervedi, S., Aggrawal, S., Ahmed, N., 2005. Hepatoprotective studies on Phyllanthus niruri paracetamol induced liver cell damage in albino mice. Experimental Medicine, 12(4):211-212.

[33] Willianson, E.M., Okpako, D.T., Evans, F.J., 1996. Selection, Preparation and Pharmacological Evaluation of Plant Material. John Wiley, England.

Open peer comments: Debate/Discuss/Question/Opinion


Dr.Balamurugan@Annamalai university<dnabala@rediffmail.com>

2011-02-05 13:32:03

Dear Respected Prof

Thank you for your kind correction.....

Dr. Nahida@Kashmir University<n.tabassum.uk@gmail .com>

2010-10-15 15:11:01

To Whom it may concern,

Kindly note the name of the Journal in Reference No: 32,

Tabassum, N., Chattervedi, S., Aggrawal, S., Ahmed, N., 2005. Hepatoprotective studies on Phyllanthus niruri paracetamol induced liver cell damage in albino mice. Experimental Medicine, 12(4):211-212.

is JK Practitioner and not Experimental Medicine which is a section of the Journal in wich Experimental Work is published.

I hope this mistake will be rectified.

With Regards,
Dr. Nahida tabassum, Deptt. of Pharmaceutical Sciences,
Kashmir University,
J&k, India-190006

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