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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.9 P.739~746

10.1631/jzus.B0820127


Relationships among magnetic resonance imaging, histological findings, and IGF-I in steroid-induced osteonecrosis of the femoral head in rabbits


Author(s):  Xing-can CHEN, Jian WENG, Xue-qun CHEN, Ji-zeng DU, Miao-ping ZHU, Yong-qing PAN, Miao LIU

Affiliation(s):  Division of Neurobiology and Physiology, Institute of Neuroscience, School of Medicine, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   genius1174@163.com

Key Words:  Dexamethasone (DEX), Insulin-like growth factor-I (IGF-I), Magnetic resonance imaging (MRI), Osteonecrosis of the femoral head, Pathology


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Xing-can CHEN, Jian WENG, Xue-qun CHEN, Ji-zeng DU, Miao-ping ZHU, Yong-qing PAN, Miao LIU. Relationships among magnetic resonance imaging, histological findings, and IGF-I in steroid-induced osteonecrosis of the femoral head in rabbits[J]. Journal of Zhejiang University Science B, 2008, 9(9): 739~746.

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author="Xing-can CHEN, Jian WENG, Xue-qun CHEN, Ji-zeng DU, Miao-ping ZHU, Yong-qing PAN, Miao LIU",
journal="Journal of Zhejiang University Science B",
volume="9",
number="9",
pages="739~746",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820127"
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%0 Journal Article
%T Relationships among magnetic resonance imaging, histological findings, and IGF-I in steroid-induced osteonecrosis of the femoral head in rabbits
%A Xing-can CHEN
%A Jian WENG
%A Xue-qun CHEN
%A Ji-zeng DU
%A Miao-ping ZHU
%A Yong-qing PAN
%A Miao LIU
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 9
%P 739~746
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820127

TY - JOUR
T1 - Relationships among magnetic resonance imaging, histological findings, and IGF-I in steroid-induced osteonecrosis of the femoral head in rabbits
A1 - Xing-can CHEN
A1 - Jian WENG
A1 - Xue-qun CHEN
A1 - Ji-zeng DU
A1 - Miao-ping ZHU
A1 - Yong-qing PAN
A1 - Miao LIU
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 9
SP - 739
EP - 746
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820127


Abstract: 
Objective: To study the relationships among magnetic resonance imaging (MRI), histological findings, and insulin-like growth factor-I (IGF-I) in steroid-induced osteonecrosis of the femoral head in rabbits. Methods: Thirty rabbits were randomly divided into experimental Group A (n=15) and control Group B (n=15). The 7.5 mg/kg (2 ml) of dexamethasone (DEX) and physiological saline (2 ml) were injected into the right gluteus medius muscle twice at one-week intervals in animals of Groups A and B, respectively. At 4, 8 and 16 weeks after obtaining an MRI, the rabbits were sacrificed and the femoral head from one side was removed for histological study of lacunae empty of osteocytes, subchondral vessels, and size of fat cells under microscopy, and the femoral head from the other side was removed for enzyme-linked immunoadsorbent assay (ELISA) for IGF-I. Results: At 4, 8 and 16 weeks after treatment, no necrotic lesions were detected in Group B, while they were detected in Group A. Light microscopy revealed that the fat cells of the marrow cavity were enlarged, subchondral vessels were evidently decreased, and empty bone lacunae were clearly increased. The IGF-I levels in Group A were significantly higher than those in Group B. At 8 weeks after the DEX injection, the MRI of all 20 femora showed an inhomogeneous, low signal intensity area in the femoral head, and at 16 weeks, the findings of all 10 femora showed a specific “line-like sign”. The MRI findings of all femora in Group B were normal. Conclusion: MRI is a highly sensitive means of diagnosing early experimental osteonecrosis of the femoral head. However, the abnormal marrow tissues appeared later than 4 weeks when the expression of IGF-I increased. This reparative factor has an early and important role in response to steroid-induced osteonecrosis of the femoral head, and provides a theoretical foundation for understanding the pathology and designing new therapies.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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