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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.5 P.365-371

http://doi.org/10.1631/jzus.B1000348


Association between moderately oxidized low-density lipoprotein and high-density lipoprotein particle subclass distribution in hemodialyzed and post-renal transplant patients


Author(s):  Elżbieta Kimak, Magdalena Hałabiś, Iwona Baranowicz-Gąszczyk, Janusz Solski, Andrzej Książek

Affiliation(s):  Department of Laboratory Diagnostics, Medical University of Lublin, ul. Chodźki 1, 20-093 Lublin, Poland, Department of Nephrology, Medical University of Lublin, Jaczewskiego 8, 20-954 Lublin, Poland

Corresponding email(s):   elzbieta.kimak@wp.pl, magdalenahalabis@gmail.com

Key Words:  Lipids, Lipoproteins, Paraoxonase-1 (PON-1) activity, Oxidized low-density lipoprotein (ox-LDL), High-density lipoprotein (HDL) particles, Post-renal transplant, Hemodialysis


Elżbieta Kimak, Magdalena Hałabiś, Iwona Baranowicz-Gąszczyk, Janusz Solski, Andrzej Książek. Association between moderately oxidized low-density lipoprotein and high-density lipoprotein particle subclass distribution in hemodialyzed and post-renal transplant patients[J]. Journal of Zhejiang University Science B, 2011, 12(5): 365-371.

@article{title="Association between moderately oxidized low-density lipoprotein and high-density lipoprotein particle subclass distribution in hemodialyzed and post-renal transplant patients",
author="Elżbieta Kimak, Magdalena Hałabiś, Iwona Baranowicz-Gąszczyk, Janusz Solski, Andrzej Książek",
journal="Journal of Zhejiang University Science B",
volume="12",
number="5",
pages="365-371",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000348"
}

%0 Journal Article
%T Association between moderately oxidized low-density lipoprotein and high-density lipoprotein particle subclass distribution in hemodialyzed and post-renal transplant patients
%A Elżbieta Kimak
%A Magdalena Hałabiś
%A Iwona Baranowicz-Gąszczyk
%A Janusz Solski
%A Andrzej Książek
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 5
%P 365-371
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000348

TY - JOUR
T1 - Association between moderately oxidized low-density lipoprotein and high-density lipoprotein particle subclass distribution in hemodialyzed and post-renal transplant patients
A1 - Elżbieta Kimak
A1 - Magdalena Hałabiś
A1 - Iwona Baranowicz-Gąszczyk
A1 - Janusz Solski
A1 - Andrzej Książek
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 5
SP - 365
EP - 371
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000348


Abstract: 
Disturbances in the metabolism of lipoprotein profiles and oxidative stress in hemodialyzed (HD) and post-renal transplant (Tx) patients are proatherogenic, but elevated concentrations of plasma high-density lipoprotein (HDL) reduce the risk of cardiovascular disease. We investigated the concentrations of lipid, lipoprotein, HDL particle, oxidized low-density lipoprotein (ox-LDL) and anti-ox-LDL, and paraoxonase-1 (PON-1) activity in HD (n=33) and Tx (n=71) patients who were non-smokers without active inflammatory disease, liver disease, diabetes, or malignancy. HD patients had moderate hypertriglyceridemia, normocholesterolemia, low HDL-C, apolipoprotein A-I (apoA-I) and HDL particle concentrations as well as PON-1 activity, and increased ox-LDL and anti-ox-LDL levels. Tx patients had hypertriglyceridemia, hypercholesterolemia, moderately decreased HDL-C and HDL particle concentrations and PON-1 activity, and moderately increased ox-LDL and anti-ox-LDL levels as compared to the reference, but ox-LDL and anti-ox-LDL levels and PON-1 activity were more disturbed in HD patients. However, in both patient groups, lipid and lipoprotein ratios (total cholesterol (TC)/HDL-C, LDL-C/HDL-C, triglyceride (TG)/HDL-C, HDL-C/non-HDL-C, apoA-I/apoB, HDL-C/apoA-I, TG/HDL) were atherogenic. The Spearman’s rank coefficient test showed that the concentration of ox-LDL correlated positively with HDL particle level (R=0.363, P=0.004), and negatively with TC (R=−0.306, P=0.012), LDL-C (R=−0.283, P=0.020), and non-HDL-C (R=−0.263, P=0.030) levels in Tx patients. Multiple stepwise forward regression analysis in Tx patients demonstrated that ox-LDL concentration, as an independent variable, was associated significantly positively with HDL particle level. The results indicated that ox-LDL and decreased PON-1 activity in Tx patients may give rise to more mildly-oxidized HDLs, which are less stable, easily undergo metabolic remodeling, generate a greater number of smaller pre-β-HDL particles, and thus accelerate reverse cholesterol transport, which may be beneficial for Tx patients. Further studies are necessary to confirm this.

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Reference

[1]An, W.S., Kim, S.E., Kim, K.H., Bae, H.R., Rha, S.H., 2009. Association between oxidized LDL to LDL ratio, HDL and vascular calcification in the feet of hemodialysis patients. J. Korean Med. Sci., 24 (Suppl. 1):S115-S120.

[2]Chen, J.X., Zhou, J.F., Shen, H.C., 2005. Oxidative stress and damage induced by abnormal free radical reactions and IgA nephropathy. J. Zhejiang Univ. Sci. B, 6(1):61-68.

[3]Cofan, F., Cofan, M., Campos, B., Guerra, R., Campistol, J.M., Oppenheimer, F., 2005. Effect of calcineurin inhibitors on low-density lipoprotein oxidation. Transplant. Proc., 37(9):3791-3793.

[4]Draganov, D., Teiber, J.F., Speelman, A., 2005. Human paraoxonases (PON1, PON2, and PON3) are lactonases with overlapping and distinct substrate specificities. J. Lipid Res., 46(6):1239-1247.

[5]Furlong, C.E., Richter, R.J., Seidel, S.L., Costa, L.G., Motulsky, A.G., 1989. Spectrophotometric assays for the enzymatic hydrolysis of the active metabolities of chlorpyrifos and parathion by plasma paraoxonase/arylesterase. Anal. Biochem., 180(2):242-247.

[6]Gao, X., Jayaraman, S., Gursky, O., 2008. Mild oxidation promotes and advanced oxidation impairs remodeling of human high-density lipoprotein in vitro. J. Mol. Biol., 376(4):997-1007.

[7]Guha, M., Gao, X., Jayaraman, S., Gursky, O., 2008. Correlation of structural stability with functional remodeling of high-density lipoproteins: the importance of being disordered. Biochemistry, 47(44):11393-11397.

[8]Joy, T., Hegele, R.A., 2008. Is raising HDL a futile strategy for atheroprotection? Nat. Rev. Drug Discov., 7(2):143-155.

[9]Kimak, E., Solski, J., Baranowicz-Gąszczyk, I., Książek, A., 2008. Serum lipid, leptin, parameters of oxidative stress and PON-1 activity in post-renal transplant patients. Ann. UMCS Pharm., 21(1):355-360.

[10]Kimak, E., Hałabiś, M., Baranowicz-Gąszczyk, I., 2010. Relationships between serum lipid, lipoprotein, triglyceride-rich lipoprotein, and high-density lipoprotein particle concentrations in post-renal transplant patients. J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.), 11(4):249-257.

[11]Krishnaswamy, P.R., Rao, A., Murali, W., Ballal, S., 2006. Paraoxonase activity to oxidized-LDL in chronic renal failure patients on renal replacement therapy. Indian J. Clin. Biochem., 21(2):173-176.

[12]Moradi, H., Pahl, M.V., Elahimehr, R., Vaziri, N.D., 2009. Impaired antioxidant activity of high-density lipoprotein in chronic kidney disease. Transl. Res., 153(2):77-85.

[13]Moreno, J.M., Ruiz, M.C., Ruiz, N., Gomez, I., Vargaz, F., Asensio, C., Osuna, A., 2005. Modulation factors of oxidative status in stable renal transplantation. Transplant. Proc., 37(3):1428-1430.

[14]Pirillo, A., Uboldi, P., Pappalardo, G., Kuhn, H., Catapano, A.L., 2007. Modification of HDL3 by mild oxidative stress increases ATP-binding cassette transporter 1-mediated cholesterol efflux. Cardiovas. Res., 75(3):566-574.

[15]Prakash, M., Phani, N.M., Kavya, R., Supriya, M., 2010. Paraoxonase: its antiatherogenic role in chronic renal failure. Indian J. Nephrol., 20(1):9-14.

[16]Rizzo, M., Kotur-Stevuljevic, J., Berneis, K., Spinas, G., Rini, G.B., Jelic-Ivanovic, Z., Spasojevic-Kalimanovska, V., Vekic, J., 2009. Atherogenic dyslipidemia and oxidative stress: a new look. Transl. Res., 153(5):217-223.

[17]Sakuma, N., Saeki, T., Ito, T., Hibino, T., Suzuki, S., Kunimatsu, M., Yamamoto, K., Kitada, S., 2010. HDL2 can inhibit further oxidative modification of partially oxidized LDL. J. Atheroscler. Thromb., 17(3):229-234.

[18]Samouilidou, E., Karpouza, A., Grapsa, E., Tzanatou-Exarchou, H., 2010. Serum oxidized LDL is inversely associated with HDL2-cholesterol subclass in renal failure patients on hemodialysis. Nephron Clin. Pract., 115(4):289-294.

[19]Shuhei, N., Söderlund, S., Jauhiainen, M., Taskinen, M.R., 2010. Effect of HDL composition and particle size on the resistance of HDL to the oxidation. Lipids Health Dis., 9(1):104-114.

[20]Stanisz, A., 2000. Approachable Statistical Course Based on STATISTICA PL Program on Medical Examples. Vol. II, Krakow, Poland, p.129-158 (in Polish).

[21]Sviridov, D., Mukhamedova, N., Remaley, A.T., Chin-Dusting, J., Nestel, P., 2008. Antiatherogenic functionality of high density lipoprotein: how much versus how good. J. Atheroscler. Thromb., 15(2):52-62.

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