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CLC number: S435.111.4+1

On-line Access: 2018-01-11

Received: 2017-07-13

Revision Accepted: 2017-11-05

Crosschecked: 2017-12-18

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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.1 P.79-84

http://doi.org/10.1631/jzus.B1700336


Physical interactions and mutational analysis of MoYpt7 in Magnaporthe oryzae


Author(s):  Lu-Yao Huang, Min Wu, Xiao-Yun Yu, Lin Li, Fu-Cheng Lin, Xiao-Hong Liu

Affiliation(s):  State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   xhliu@zju.edu.cn

Key Words:  Bimolecular fluorescence complementation, Immunoprecipitation, MoGdi1, MoYpt7


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Lu-Yao Huang, Min Wu, Xiao-Yun Yu, Lin Li, Fu-Cheng Lin, Xiao-Hong Liu. Physical interactions and mutational analysis of MoYpt7 in Magnaporthe oryzae[J]. Journal of Zhejiang University Science B, 2018, 19(1): 79-84.

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author="Lu-Yao Huang, Min Wu, Xiao-Yun Yu, Lin Li, Fu-Cheng Lin, Xiao-Hong Liu",
journal="Journal of Zhejiang University Science B",
volume="19",
number="1",
pages="79-84",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700336"
}

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%T Physical interactions and mutational analysis of MoYpt7 in Magnaporthe oryzae
%A Lu-Yao Huang
%A Min Wu
%A Xiao-Yun Yu
%A Lin Li
%A Fu-Cheng Lin
%A Xiao-Hong Liu
%J Journal of Zhejiang University SCIENCE B
%V 19
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%@ 1673-1581
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700336

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T1 - Physical interactions and mutational analysis of MoYpt7 in Magnaporthe oryzae
A1 - Lu-Yao Huang
A1 - Min Wu
A1 - Xiao-Yun Yu
A1 - Lin Li
A1 - Fu-Cheng Lin
A1 - Xiao-Hong Liu
J0 - Journal of Zhejiang University Science B
VL - 19
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EP - 84
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1700336


Abstract: 
In this study, we analyzed the physical interactions of the dominant negative isoform of moYpt7. Our results show that moYpt7 interacts with moGdi1. The dominant negative isoform of moYpt7 (dominant negative isoform, N125I) is essential for colony morphology, conidiation, and pathogenicity in the rice blast fungus. These results further demonstrate the biological functions of moYpt7 in Magnaporthe oryzae.

稻瘟病菌MoYpt7互作蛋白和点突变的研究

目的:研究MoYpt7与MoGdi1的互作关系以及定点突变体MoYpt7N125I的生物学功能.
创新点:补充了MoYpt7在稻瘟病菌致病过程中的生物学功能.
方法:采用双分子荧光互补和免疫共沉淀的方法检测MoYpt7与MoGdi1的相互作用.利用点突变的方法,分析MoYpt7的鸟嘌呤二核苷酸磷酸(GDP)结合形式的生物学功能.
结论:MoYpt7与Rab-GDP解离抑制因子的一个同源物MoGdi1相互作用,获得了MoYpt7的GDP结合形式的过表达突变体.该突变体菌落形态发生变化,产孢能力和致病性显著下降.同时,稻瘟病菌MoYpt7N125I的亚细胞定位发生变化.

关键词:双分子荧光互补;免疫沉淀;MoGdi1;MoYpt7

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