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CLC number: S816.3

On-line Access: 2018-09-30

Received: 2017-10-08

Revision Accepted: 2017-12-28

Crosschecked: 2018-09-10

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Citations:  Bibtex RefMan EndNote GB/T7714


Wei-fen Li


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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.10 P.785-795


Glycyrrhizic acid activates chicken macrophages and enhances their Salmonella-killing capacity in vitro

Author(s):  Bai-kui Wang, Yu-long Mao, Li Gong, Xin Xu, Shou-qun Jiang, Yi-bing Wang, Wei-fen Li

Affiliation(s):  Key Laboratory of Animal Molecular Nutrition of Education of Ministry, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   wfli@zju.edu.cn

Key Words:  Glycyrrhizic acid, Chicken macrophage, Macrophage activation, Salmonella Typhimurium, Nuclear factor κ, B (NF-κ, B), c-Jun N-terminal kinase (JNK)

Bai-kui Wang, Yu-long Mao, Li Gong, Xin Xu, Shou-qun Jiang, Yi-bing Wang, Wei-fen Li. Glycyrrhizic acid activates chicken macrophages and enhances their Salmonella-killing capacity in vitro[J]. Journal of Zhejiang University Science B, 2018, 19(10): 785-795.

@article{title="Glycyrrhizic acid activates chicken macrophages and enhances their Salmonella-killing capacity in vitro",
author="Bai-kui Wang, Yu-long Mao, Li Gong, Xin Xu, Shou-qun Jiang, Yi-bing Wang, Wei-fen Li",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Glycyrrhizic acid activates chicken macrophages and enhances their Salmonella-killing capacity in vitro
%A Bai-kui Wang
%A Yu-long Mao
%A Li Gong
%A Xin Xu
%A Shou-qun Jiang
%A Yi-bing Wang
%A Wei-fen Li
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 10
%P 785-795
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700506

T1 - Glycyrrhizic acid activates chicken macrophages and enhances their Salmonella-killing capacity in vitro
A1 - Bai-kui Wang
A1 - Yu-long Mao
A1 - Li Gong
A1 - Xin Xu
A1 - Shou-qun Jiang
A1 - Yi-bing Wang
A1 - Wei-fen Li
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 10
SP - 785
EP - 795
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700506

Objective: Salmonella enterica remains a major cause of food-borne disease in humans, and Salmonella Typhimurium (ST) contamination of poultry products is a worldwide problem. Since macrophages play an essential role in controlling Salmonella infection, the aim of this study was to evaluate the effect of glycyrrhizic acid (GA) on immune function of chicken HD11 macrophages. Methods: Chicken HD11 macrophages were treated with GA (0, 12.5, 25, 50, 100, 200, 400, or 800 μg/ml) and lipopolysaccharide (LPS, 500 ng/ml) for 3, 6, 12, 24, or 48 h. Evaluated responses included phagocytosis, bacteria-killing, gene expression of cell surface molecules (cluster of differentiation 40 (CD40), CD80, CD83, and CD197) and antimicrobial effectors (inducible nitric oxide synthase (iNOS), NADPH oxidase-1 (NOX-1), interferon-γ (IFN-γ), LPS-induced tumor necrosis factor (TNF)-α factor (LITAF), interleukin-6 (IL-6), and IL-10), and production of nitric oxide (NO) and hydrogen peroxide (H2O2). Results: GA increased the internalization of both fluorescein isothiocyanate (FITC)-dextran and ST by HD11 cells and markedly decreased the intracellular survival of ST. We found that the messenger RNA (mRNA) expression of cell surface molecules (CD40, CD80, CD83, and CD197) and cytokines (IFN-γ, IL-6, and IL-10) of HD11 cells was up-regulated following GA exposure. The expression of iNOS and NOX-1 was induced by GA and thereby the productions of NO and H2O2 in HD11 cells were enhanced. Notably, it was verified that nuclear factor-κb (NF-κ;b) and c-Jun N-terminal kinase (JNK) pathways were responsible for GA-induced synthesis of NO and IFN-γ gene expression. Conclusions: Taken together, these results suggested that GA exhibits a potent immune regulatory effect to activate chicken macrophages and enhances Salmonella-killing capacity.


方法:以不同浓度的甘草酸(0、12.5、25、50、100、200、400和800 µg/ml)处理鸡巨噬细胞系HD11,采用荧光定量聚合酶链式反应(qPCR)和一氧化氮及过氧化氢测定试剂盒评价甘草酸对鸡巨噬细胞活化和免疫的影响,采用流式细胞技术和涂板计数法测定鸡巨噬细胞吞噬和杀菌能力.


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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[36]List of electronic supplementary materials

[37]Fig. S1 In vitro antibacterial activity of glycyrrhizic acid against Salmonella Typhimurium

[38]Fig. S2 Effect of glycyrrhizic acid on Salmonella Typhimurium virulence gene expression in vitro

[39]Table S1 List of real-time PCR primers

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