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CLC number: Q939.99

On-line Access: 2019-01-07

Received: 2018-03-31

Revision Accepted: 2018-07-22

Crosschecked: 2019-01-02

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Citations:  Bibtex RefMan EndNote GB/T7714


Man Zhang


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Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.1 P.49-58


Factors influencing the accuracy of the denitrifier method for determining the oxygen isotopic composition of nitrate

Author(s):  Man Zhang, Jia-chun Shi, Lao-sheng Wu

Affiliation(s):  Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang Provincial Key Laboratory of Agricultural and Environment, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   jcshi@zju.edu.cn

Key Words:  Denitrifier method, Nitrate, δ15N, δ18O, Dissolved oxygen, δ18O contraction

Man Zhang, Jia-chun Shi, Lao-sheng Wu. Factors influencing the accuracy of the denitrifier method for determining the oxygen isotopic composition of nitrate[J]. Journal of Zhejiang University Science B, 2019, 20(1): 49-58.

@article{title="Factors influencing the accuracy of the denitrifier method for determining the oxygen isotopic composition of nitrate",
author="Man Zhang, Jia-chun Shi, Lao-sheng Wu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Factors influencing the accuracy of the denitrifier method for determining the oxygen isotopic composition of nitrate
%A Man Zhang
%A Jia-chun Shi
%A Lao-sheng Wu
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 1
%P 49-58
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1800197

T1 - Factors influencing the accuracy of the denitrifier method for determining the oxygen isotopic composition of nitrate
A1 - Man Zhang
A1 - Jia-chun Shi
A1 - Lao-sheng Wu
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 1
SP - 49
EP - 58
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1800197

The denitrifier method is widely used as a novel pretreatment method for the determination of nitrogen and oxygen isotope ratios as it can provide quantitative and high-sensitivity measurements. Nevertheless, the method is limited by relatively low measurement accuracy for δ18O. In this study, we analyzed the factors influencing the accuracy of δ18O determination, and then systematically investigated the effects of dissolved oxygen concentrations and nitrate sample sizes on estimates of the δ15N and δ18O of nitrate reference materials. The δ18O contraction ratio was used to represent the relationship between the measured difference and true difference between two reference materials. We obtained the following main results: (1) a gas-liquid ratio of 3:10 (v/v) in ordinary triangular flasks and a shaking speed of 120 r/min produced an optimal range (1.9 to 2.6 mg/L) in the concentration of dissolved oxygen for accurately determining δ18O, and (2) the δ18O contraction ratio decreased as nitrate sample size decreased within a certain range (1.0 to 0.1 μmol). Our results suggested that δ18O contraction is influenced mainly by dissolved oxygen concentrations in pure culture, and provided a model for improving the accuracy of oxygen isotope analysis.


方法:通过控制培养瓶内的气液比(1:5、3:10、2:5和3:5)和摇床转速(60和120 r/min)来调节纯培养时的溶解氧浓度,并设计不同的硝酸盐进样量(0.05~1.00 µmol),由硝酸盐标准物质的测试结果判断适宜的溶解氧浓度和硝酸盐进样量.
结论:标准物质间的δ18O差值收缩主要受溶解氧浓度的影响.根据氧同位素测试结果的精度和δ18O差值收缩率的大小,得出以下结论:当普通三角瓶内的气液比为3:10,摇床转速为120 r/min时的溶解氧浓度最佳(1.9~2.6 mg/L),0.2~0.4 nmol的硝酸盐进样量最适宜.


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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