CLC number: Q516
On-line Access: 2019-01-07
Received: 2017-12-01
Revision Accepted: 2018-04-19
Crosschecked: 2018-12-05
Cited: 0
Clicked: 6622
Yixian Quah, Nor Ismaliza Mohd Ismail, Jillian Lean Sim Ooi, Yang Amri Affendi, Fazilah Abd Manan, Lai-Kuan Teh, Fai-Chu Wong, Tsun-Thai Chai. Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum[J]. Journal of Zhejiang University Science B, 2019, 20(1): 59-70.
@article{title="Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum",
author="Yixian Quah, Nor Ismaliza Mohd Ismail, Jillian Lean Sim Ooi, Yang Amri Affendi, Fazilah Abd Manan, Lai-Kuan Teh, Fai-Chu Wong, Tsun-Thai Chai",
journal="Journal of Zhejiang University Science B",
volume="20",
number="1",
pages="59-70",
year="2019",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700586"
}
%0 Journal Article
%T Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum
%A Yixian Quah
%A Nor Ismaliza Mohd Ismail
%A Jillian Lean Sim Ooi
%A Yang Amri Affendi
%A Fazilah Abd Manan
%A Lai-Kuan Teh
%A Fai-Chu Wong
%A Tsun-Thai Chai
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 1
%P 59-70
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700586
TY - JOUR
T1 - Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum
A1 - Yixian Quah
A1 - Nor Ismaliza Mohd Ismail
A1 - Jillian Lean Sim Ooi
A1 - Yang Amri Affendi
A1 - Fazilah Abd Manan
A1 - Lai-Kuan Teh
A1 - Fai-Chu Wong
A1 - Tsun-Thai Chai
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 1
SP - 59
EP - 70
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700586
Abstract: Globally, peptide-based anticancer therapies have drawn much attention. Marine organisms are a reservoir of anticancer peptides that await discovery. In this study, we aimed to identify cytotoxic oligopeptides from Sarcophyton glaucum. Peptides were purified from among the S. glaucum hydrolysates produced by alcalase, chymotrypsin, papain, and trypsin, guided by a methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay on the human cervical cancer (HeLa) cell line for cytotoxicity evaluation. Purification techniques adopted were membrane ultrafiltration, gel filtration chromatography, solid phase extraction (SPE), and reversed-phase high-performance liquid chromatography (RP-HPLC). Purified peptides were identified by de novo peptide sequencing. From papain hydrolysate, three peptide sequences were identified: AGAPGG, AERQ, and RDTQ (428.45, 502.53, and 518.53 Da, respectively). Peptides synthesized from these sequences exhibited cytotoxicity on heLa cells with median effect concentration (EC50) values of 8.6, 4.9, and 5.6 mmol/L, respectively, up to 5.8-fold stronger than the anticancer drug 5-fluorouracil. When tested at their respective EC50, AGAPGG, AERQ, and RDTQ showed only 16%, 25%, and 11% cytotoxicity to non-cancerous Hek293 cells, respectively. In conclusion, AERQ, AGAPGG, and RDTQ are promising candidates for future development as peptide-based anticancer drugs.
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[46]List of electronic supplementary materials
[47]Fig. S1 A representative SPE elution prolife of GF3
[48]Fig. S2 Peptide content of SPE fractions
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