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Bio-Design and Manufacturing  2022 Vol.5 No.2 P.348-357

http://doi.org/10.1007/s42242-021-00155-2


Coaxial bioprinted microfibers with mesenchymal stem cells for glioma microenvironment simulation


Author(s):  Zhizhong Jin, Xinda Li, Boxun Liu, Xinru Yan, Shuai Han, Tao Xu & Anhua Wu

Affiliation(s):  Department of Neurosurgery, The First Hospital of China Medical University, Shenyang 110001, China; more

Corresponding email(s):   taoxu@mail.tsinghua.edu.cn, ahwu@cmu.edu.cn

Key Words:  Coaxial bioprinting, GBM microenvironment, MSC, Coculture


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Zhizhong Jin, Xinda Li, Boxun Liu, Xinru Yan, Shuai Han, Tao Xu & Anhua Wu . Coaxial bioprinted microfibers with mesenchymal stem cells for glioma microenvironment simulation[J]. Journal of Zhejiang University Science D, 2022, 5(2): 348-357.

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doi="10.1007/s42242-021-00155-2"
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Abstract: 
Due to their special anatomical and physiological features, central nervous system diseases still presented challenges, despite the fact that some advances have been made in early diagnosis and precision medicine. One of the complexities in treating tumors is the tumor microenvironment, which includes mesenchymal stem cells (MSCs) that exhibit tumor tropism and can be used for cell therapy. However, whether MSCs promote or suppress gliomas is still unclear, especially in glioma microenvironments. In this study, a coaxial microfiber was designed to mimic the tumor microenvironment and to reveal the effect of MSCs on glioma cells. The fiber shell was composed of MSCs and alginate, and the core was filled with U87MG (glioblastoma) cells and gelatin methacrylate. This Shell-MSC/Core-U87MG microenvironment improved the proliferation, survival, invasion, metastasis, and drug resistance of glioma cells, while simultaneously maintaining the stemness of glioma cells. In summary, coaxial extrusion bioprinted Shell-MSC/Core-U87MG microfiber is an ideal platform for tumor and stromal cell coculture to observe tumor biological behavior in vitro.

中国医大一院吴安华、清华大学徐弢等|同轴生物打印间充质干细胞微纤维模拟肿瘤微环境

本研究论文聚焦同轴生物打印间充质干细胞微纤维模拟肿瘤微环境。尽管中枢神经系统疾病在早期诊断和精准医学方面取得了一些进展,但由于其特殊的解剖结构和生理特征,仍面临巨大的挑战。肿瘤治疗的复杂性之一体现在肿瘤微环境的成分复杂,包括表现出肿瘤倾向性并可用于细胞治疗的间充质干细胞(MSCs)。在胶质瘤微环境中,MSCs是否促进或抑制胶质瘤仍不清楚。本研究设计了一种同轴微纤维来模拟肿瘤微环境,以揭示MSCs对胶质瘤细胞的影响。纤维外壳由间充质干细胞和海藻酸盐组成,纤维核心充满U87MG(胶质母细胞瘤)细胞和甲基丙烯酸明胶。这种外壳-MSC/内芯-U87MG微环境提高了胶质瘤细胞的增殖、生存、侵袭、转移和耐药能力,同时保持了保证胶质瘤细胞无限增殖能力的肿瘤细胞干性,提示MSCs在人体内的肿瘤微环境中同样可能有促瘤的作用,是需要临床考虑的因素之一。综上所述,同轴挤压生物打印Shell-MSC/Core-U87MG超细纤维是肿瘤和基质细胞共培养、观察肿瘤生物学行为的理想平台,也为其他具有复杂肿瘤微环境组分的肿瘤提供了共培养新思路,具有一定的临床前景和指导作用。

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