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On-line Access: 2024-03-26
Received: 2023-07-07
Revision Accepted: 2023-12-13
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Biofabrication of nanocomposite-based scaffolds containing human bone extracellular matrix for the differentiation of skeletal stem and progenitor cells
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Yang-Hee Kim, Janos M. Kanczler, Stuart Lanham, Andrew Rawlings, Marta Roldo, Gianluca Tozzi, Jonathan I. Dawson, Gianluca Cidonio & Richard O. C. Oreffo. Biofabrication of nanocomposite-based scaffolds containing human bone extracellular matrix for the differentiation of skeletal stem and progenitor cells[J]. Journal of Zhejiang University Science D, 2024, 7(2): 121-136.
@article{title="Biofabrication of nanocomposite-based scaffolds containing human
bone extracellular matrix for the differentiation of skeletal stem
and progenitor cells",
author="Yang-Hee Kim, Janos M. Kanczler, Stuart Lanham, Andrew Rawlings, Marta Roldo, Gianluca Tozzi, Jonathan I. Dawson, Gianluca Cidonio & Richard O. C. Oreffo",
journal="Journal of Zhejiang University Science D",
volume="7",
number="2",
pages="121-136",
year="2024",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-023-00265-z"
}
%0 Journal Article
%T Biofabrication of nanocomposite-based scaffolds containing human
bone extracellular matrix for the differentiation of skeletal stem
and progenitor cells
%A Yang-Hee Kim
%A Janos M. Kanczler
%A Stuart Lanham
%A Andrew Rawlings
%A Marta Roldo
%A Gianluca Tozzi
%A Jonathan I. Dawson
%A Gianluca Cidonio & Richard O. C. Oreffo
%J Journal of Zhejiang University SCIENCE D
%V 7
%N 2
%P 121-136
%@ 1869-1951
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-023-00265-z
TY - JOUR
T1 - Biofabrication of nanocomposite-based scaffolds containing human
bone extracellular matrix for the differentiation of skeletal stem
and progenitor cells
A1 - Yang-Hee Kim
A1 - Janos M. Kanczler
A1 - Stuart Lanham
A1 - Andrew Rawlings
A1 - Marta Roldo
A1 - Gianluca Tozzi
A1 - Jonathan I. Dawson
A1 - Gianluca Cidonio & Richard O. C. Oreffo
J0 - Journal of Zhejiang University Science D
VL - 7
IS - 2
SP - 121
EP - 136
%@ 1869-1951
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-023-00265-z
Abstract: Autograft or metal implants are routinely used in skeletal repair. However, they fail to provide long-term clinical resolution,
necessitating a functional biomimetic tissue engineering alternative. The use of native human bone tissue for synthesizing a
biomimetic material ink for three-dimensional (3D) bioprinting of skeletal tissue is an attractive strategy for tissue regeneration.
Thus, human bone extracellular matrix (bone-ECM) offers an exciting potential for the development of an appropriate
microenvironment for human bone marrow stromal cells (HBMSCs) to proliferate and differentiate along the osteogenic
lineage. In this study, we engineered a novel material ink (LAB) by blending human bone-ECM (B) with nanoclay (L,
Laponite®) and alginate (A) polymers using extrusion-based deposition. The inclusion of the nanofiller and polymeric material
increased the rheology, printability, and drug retention properties and, critically, the preservation of HBMSCs viability upon
printing. The composite of human bone-ECM-based 3D constructs containing vascular endothelial growth factor (VEGF)
enhanced vascularization after implantation in an ex vivo chick chorioallantoic membrane (CAM) model. The inclusion of
bone morphogenetic protein-2 (BMP-2) with the HBMSCs further enhanced vascularization and mineralization after only
seven days. This study demonstrates the synergistic combination of nanoclay with biomimetic materials (alginate and boneECM) to support the formation of osteogenic tissue both in vitro and ex vivo and offers a promising novel 3D bioprinting
approach to personalized skeletal tissue repair.
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