Is there a role of TNFR1 in acute lung injury cases associated with extracorporeal circulation?
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Yu Zhao, Chong-wei Zhang, Wen-jing Zhou, Jiao Chen, Nan-fu Luo, Li-na Gong, Lei Du, Jing Zhou. Is there a role of TNFR1 in acute lung injury cases associated with extracorporeal circulation?[J]. Journal of Zhejiang University Science B, 2014, 15(3): 281-288.
@article{title="Is there a role of TNFR1 in acute lung injury cases associated with extracorporeal circulation?",
author="Yu Zhao, Chong-wei Zhang, Wen-jing Zhou, Jiao Chen, Nan-fu Luo, Li-na Gong, Lei Du, Jing Zhou",
journal="Journal of Zhejiang University Science B",
volume="15",
number="3",
pages="281-288",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300147"
}
%0 Journal Article
%T Is there a role of TNFR1 in acute lung injury cases associated with extracorporeal circulation?
%A Yu Zhao
%A Chong-wei Zhang
%A Wen-jing Zhou
%A Jiao Chen
%A Nan-fu Luo
%A Li-na Gong
%A Lei Du
%A Jing Zhou
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 3
%P 281-288
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300147
TY - JOUR
T1 - Is there a role of TNFR1 in acute lung injury cases associated with extracorporeal circulation?
A1 - Yu Zhao
A1 - Chong-wei Zhang
A1 - Wen-jing Zhou
A1 - Jiao Chen
A1 - Nan-fu Luo
A1 - Li-na Gong
A1 - Lei Du
A1 - Jing Zhou
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 3
SP - 281
EP - 288
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300147
Abstract: The signaling pathway for tumor necrosis factor-α (TNF-α) and its receptors is up-regulated during extracorporeal circulation (ECC), and recruits blood neutrophil into the lung tissue, which results in acute lung injury (ALI). In this study, we evaluated the role of tumor necrosis factor receptor 1 (TNFR1) in ECC-induced ALI by blocking TNF-α binding to TNFR1 with CAY10500. Anesthetized Sprague-Dawley (SD) rats were pretreated intravenously with phosphate buffered saline (PBS) or vehicle (0.3 ml ethanol IV) or CAY10500, and then underwent ECC for 2 h. The oxygenation index (OI) and pulmonary inflammation were assessed after ECC. OI was significantly decreased, while TNF-α and neutrophil in bronchoalveolar lavage fluid (BALF) and plasma TNF-α increased after ECC. Pretreatment of CAY10500 decreased plasma TNF-α level, but did not decrease TNF-α levels and neutrophil counts in BALF or improve OI. Lung histopathology showed significant alveolar congestion, infiltration of the leukocytes in the airspace, and increased thickness of the alveolar wall in all ECC-treated groups. CAY10500 pretreatment slightly reduced leukocyte infiltration in lungs, but did not change the wet/dry ratio in the lung tissue. Blocking TNF-α binding to TNFR1 by CAY10500 intravenously slightly mitigates pulmonary inflammation, but cannot improve the pulmonary function, indicating the limited role of TNFR1 pathway in circulating inflammatory cell in ECC-induced ALI.
Full Text:
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CLC number: R-332
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2014-02-08
Cited: 0
Clicked: 10750
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