Full Text:   <1803>

CLC number: R741

On-line Access: 

Received: 2004-05-10

Revision Accepted: 2004-09-12

Crosschecked: 0000-00-00

Cited: 5

Clicked: 4588

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.4 P.254~258


GM1 stabilizes expression of NMDA receptor subunit 1 in the ischemic hemisphere of MCAo/reperfusion rat

Author(s):  LIU Jian-ren, DING Mei-ping, WEI Er-qing, LUO Jian-hong, SONG Ying, HUANG Jian-zheng, GE Qiu-fu, HU Hua, ZHU Li-jun

Affiliation(s):  Department of Neurology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   Liujianren@medmail.com.cn

Key Words:  G(M1) ganglioside, Middle cerebral artery occlusion, Reperfusion, N-methyl-D-aspartate receptors, Rats

LIU Jian-ren, DING Mei-ping, WEI Er-qing, LUO Jian-hong, SONG Ying, HUANG Jian-zheng, GE Qiu-fu, HU Hua, ZHU Li-jun. GM1 stabilizes expression of NMDA receptor subunit 1 in the ischemic hemisphere of MCAo/reperfusion rat[J]. Journal of Zhejiang University Science B, 2005, 6(4): 254~258.

@article{title="GM1 stabilizes expression of NMDA receptor subunit 1 in the ischemic hemisphere of MCAo/reperfusion rat",
author="LIU Jian-ren, DING Mei-ping, WEI Er-qing, LUO Jian-hong, SONG Ying, HUANG Jian-zheng, GE Qiu-fu, HU Hua, ZHU Li-jun",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T GM1 stabilizes expression of NMDA receptor subunit 1 in the ischemic hemisphere of MCAo/reperfusion rat
%A LIU Jian-ren
%A DING Mei-ping
%A WEI Er-qing
%A LUO Jian-hong
%A SONG Ying
%A HUANG Jian-zheng
%A GE Qiu-fu
%A HU Hua
%A ZHU Li-jun
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 4
%P 254~258
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0254

T1 - GM1 stabilizes expression of NMDA receptor subunit 1 in the ischemic hemisphere of MCAo/reperfusion rat
A1 - LIU Jian-ren
A1 - DING Mei-ping
A1 - WEI Er-qing
A1 - LUO Jian-hong
A1 - SONG Ying
A1 - HUANG Jian-zheng
A1 - GE Qiu-fu
A1 - HU Hua
A1 - ZHU Li-jun
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 4
SP - 254
EP - 258
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0254

Objective: To determine the protective effect of monosialoganglionside (GM1) and evaluate the influence of GM1 on expression of N-methyl-D-aspartate receptor subunit 1 (NMDAR1) in Sprague-Dawley (SD) rats with focal cerebral ischemia-reperfusion (I/R). Methods: Left middle cerebral artery (MCA) was occluded by an intraluminal suture for 1 h and the brain was reperfused for 72 h in SD rats when infarct volume was measured, GM1 (10 mg/kg) was given ip (intraperitoneally) at 5 min (group A), 1 h (group B) and 2 h (group C) after MCA occlusion (MCAo). Expression of NMDAR1 was detected by Western blot at various time after reperfusion (4 h, 6 h, 24 h, 48 h and 72 h) in ischemic hemispheres of the rats with or without GM1 administered. Results: (1) Adjusted relative infarct volumes of groups A and B were significantly smaller than that of group C and the control group (P<0.01 and P<0.05, respectively). (2) Expression level of NMDAR1 was temporally high at 6 h after reperfusion, and dipped below the normal level at 72 h after reperfusion. GM1 at 5 min after MCAo significantly suppressed the expression of NMDAR1 at 6 h after reperfusion (P<0.05 vs the control). At 72 h after reperfusion, the NMDAR1 expression level of rats treated with GM1 administered (at 5 min or 2 h after MCAo) was significantly higher than that of the control (P<0.05). Conclusion: GM1 can time-dependently reduce infarct volume in rats with focal cerebral I/R partly through stabilizing the expression of NMDAR1.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Brandoli, C., Sanna, A., De Bernardi, M.A., Follesa, P., Brooker, G., Mocchetti, I., 1998. Brain-derived neurotrophic factor and basic fibroblast growth factor downregulate NMDA receptor function in cerebellar granule cells. J Neurosci, 18(19):7953-7961.

[2] Choi, D.W., Rothman, S.M., 1990. The role of glutamate neurotoxicity in hypoxic-ischemic neuronal death. Annu Rev Neurosci, 13:171-182.

[3] Collingridge, G.L., Singer, W., 1990. Excitatory amino acid receptors and synaptic plasticity. Trends Pharmacol Sci, 11(7):290-296.

[4] de Erausquin, G.A., Manev, H., Guidotti, A., Costa, E., Brooker, G., 1990. Gangliosides normalize distorted single-cell intracellular free Ca2+ dynamics after toxic doses of glutamate in cerebellar granule cells. Proc Natl Acad Sci USA, 87(20):8017-8021.

[5] Duchemin, A.M., Ren, Q., Mo, L., Neff, N.H., Hadjiconstantinou, M., 2002. GM1 ganglioside induces phosphorylation and activation of Trk and Erk in brain. J Neurochem, 81(4):696-707.

[6] Friedman, L.K., Ginsberg, M.D., Belayev, L., Busto, R., Alonso, O.F., Lin, B., Globus, M.Y., 2001. Intraischemic but not postischemic hypothermia prevents non-selective hippocampal downregulation of AMPA and NMDA receptor gene expression after global ischemia. Brain Res Mol Brain Res, 86(1-2):34-47.

[7] Garofalo, L., Cuello, A.C., 1994. Nerve growth factor and the monosialoganglioside GM1: analogous and different in vivo effects on biochemical, morphological, and behavioral parameters of adult cortically lesioned rats. Exp Neurol, 125(2):195-217.

[8] Kang, T.C., Hwang, I.K., Park, S.K., An, S.J., Yoon, D.K., Moon, S.M., Lee, Y.B., Sohn, H.S., Cho, S.S., Won, M.H., 2001. Chronological changes of N-methyl-D-aspartate receptors and excitatory amino acid carrier 1 immunoreactivities in CA1 area and subiculum after transient forebrain ischemia. J Neurocytol, 30(12):945-955.

[9] Kharlamov, A., Guidotti, A., Costa, E., Hayes, R., Armstrong, D., 1993. Semisynthetic sphingolipids prevent protein kinase C translocation and neuronal damage in the perifocal area following a photochemically induced thrombotic brain cortical lesion. J Neurosci, 13(6):2483-2494.

[10] Longa, E.Z., Weinstein, P.R., Carlson, S., Cummins, R., 1989. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke, 20(1):84-91.

[11] Luo, J., Bosy, T.Z., Wang, Y., Yasuda, R.P., Wolfe, B.B., 1996. Ontogeny of NMDAR1 subunit protein expression in five regions of rat brain. Brain Res Dev Brain Res, 92(1):10-17.

[12] Luo, J., Wang, Y., Yasuda, R.P., Dunah, A.W., Wolfe, B.B., 1997. The majority of n-methyl-d-aspartate receptor Complexes in adult rat cerebral cortex contain at least three different subunits (NMDAR1/NMDAR2A/ NMDAR2B). Mol Pharmacol, 51(1):79-86.

[13] Manev, H., Favaron, M., Vicini, S., Guidotti, A., Costa, E., 1990. Glutamate-induced neuronal death in primary cultures of cerebellar granule cells: protection by synthetic derivatives of endogenous sphingolipids. J Pharmacol Exp Ther, 252(1):419-427.

[14] Michaels, R.L., Rothman, S.M., 1990. Glutamate neurotoxicity in vitro: antagonist pharmacology and intracellular calcium concentrations. J Neurosci, 10(1):283-292.

[15] Monaghan, D.T., Bridges, R.J., Cotman, C.W., 1989. The excitatory amino acid receptors: their classes, pharmacology, and distinct properties in the function of the central nervous system. Annu Rev Pharmacol Toxicol, 29:365-402.

[16] Rossi, D.J., Oshima, T., Attwell, D., 2000. Glutamate release in severe brain ischemia is mainly by reversed uptake. Nature, 403(6767):316-321.

[17] Simon, R.P., Chen, J., Graham, S.H., 1993. GM1 ganglioside treatment of focal ischemia: a dose-response and microdialysis study. J Pharmacol Exp Ther, 265(1):24-29.

[18] Wahlestedt, C., Golanov, E., Yamamoto, S., Yee, F., Ericson, H., Yoo, H., Inturrisi, C.E., Reis, D.J., 1993. Antisense oligodeoxynucleotides to NMDA-R1 receptor channel protect cortical neurons from excitotoxicity and reduce focal ischaemic infarctions. Nature, 363(6426):260-263.

[19] Won, M.H., Kang, T., Park, S., Jeon, G., Kim, Y., Seo, J.H., Choi, E., Chung, M., Cho, S.S., 2001. The alterations of N-Methyl-D-aspartate receptor expressions and oxidative DNA damage in the CA1 area at the early time after ischemia-reperfusion insult. Neurosci Lett, 301(2):139-142.

[20] Zang, L.H., Wei, E.Q., 2003. Neuroprotective effect of ONO-1078, a leukotriene receptor antagonist, on transient global cerebral ischemia in rats. Acta Pharmacol Sin, 24(12):1241-1247.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE