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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.9 P.859~864


Preparation and in vitro studies of microencapsulated cells releasing human tissue inhibitor of metalloproteinase-2

Author(s):  JIANG Qiang, ZHANG Su-zhan, PENG Jia-ping, WANG Xu-lin

Affiliation(s):  Cancer Institute, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China

Corresponding email(s):   jiangtiger@zju.edu.cn, zhangscy@tom.com

Key Words:  Microencapsulation, Recombinant cells, Human tissue inhibitor of metalloproteinase-2, Cell culture

JIANG Qiang, ZHANG Su-zhan, PENG Jia-ping, WANG Xu-lin. Preparation and in vitro studies of microencapsulated cells releasing human tissue inhibitor of metalloproteinase-2[J]. Journal of Zhejiang University Science B, 2005, 6(9): 859~864.

@article{title="Preparation and in vitro studies of microencapsulated cells releasing human tissue inhibitor of metalloproteinase-2",
author="JIANG Qiang, ZHANG Su-zhan, PENG Jia-ping, WANG Xu-lin",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Preparation and in vitro studies of microencapsulated cells releasing human tissue inhibitor of metalloproteinase-2
%A JIANG Qiang
%A ZHANG Su-zhan
%A PENG Jia-ping
%A WANG Xu-lin
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 9
%P 859~864
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0859

T1 - Preparation and in vitro studies of microencapsulated cells releasing human tissue inhibitor of metalloproteinase-2
A1 - JIANG Qiang
A1 - ZHANG Su-zhan
A1 - PENG Jia-ping
A1 - WANG Xu-lin
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 9
SP - 859
EP - 864
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0859

Objective: To prepare microencapsulated cells releasing human tissue inhibitor of metalloproteinase-2 (TIMP-2), and investigate their biological characteristics in vitro. Methods: Chinese hamster ovary (CHO) cells were stably transfected with a human TIMP-2 expression vector, encapsulated in barium alginate microcapsules and cultured in vitro. Morphological appearance of the microcapsules was observed under a light microscope. Cell viability was assessed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Enzyme linked immunosorbent assay (ELISA) and reverse zymography were used to confirm the release of biologically active TIMP-2 from the microcapsules. Cryopreservation study of the microencapsulated cells was carried out using dimethyl sulfoxide (DMSO) as preservative agent. Results: The microcapsules appeared like a sphere with diameter of 300~600 μm. The surface of the capsule wall was clearly smooth. The microencapsulated cells survived well and kept proliferating over the 6 weeks observed. No significant difference in TIMP-2 secretion was found between encapsulated and unencapsulated cells. Reverse zymography confirmed the bioactivity of MMP (matrix metalloproteinase) inhibition of TIMP-2. The cryopreservation process did not damage the microcapsule morphology nor the viability of the cells inside. Conclusion: Microencapsulated engineered CHO cells survive at least 6 weeks after preparation in vitro, and secrete bioactive TIMP-2 freely from the microcapsules.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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