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Received: 2005-07-13

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.11 P.1076~1080


Batch and fed-batch production of butyric acid by Clostridium butyricum ZJUCB

Author(s):  HE Guo-qing, KONG Qing, CHEN Qi-he, RUAN Hui

Affiliation(s):  Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   gqhe@zju.edu.cn, kongqingg@163.com

Key Words:  Clostridium butyricum, Batch fermentation, Fed-batch fermentation, pH, Butyric acid production, Acetic acid

HE Guo-qing, KONG Qing, CHEN Qi-he, RUAN Hui. Batch and fed-batch production of butyric acid by Clostridium butyricum ZJUCB[J]. Journal of Zhejiang University Science B, 2005, 6(11): 1076~1080.

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publisher="Zhejiang University Press & Springer",

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%A HE Guo-qing
%A KONG Qing
%A CHEN Qi-he
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%DOI 10.1631/jzus.2005.B1076

T1 - Batch and fed-batch production of butyric acid by Clostridium butyricum ZJUCB
A1 - HE Guo-qing
A1 - KONG Qing
A1 - CHEN Qi-he
A1 - RUAN Hui
J0 - Journal of Zhejiang University Science B
VL - 6
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SP - 1076
EP - 1080
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2005.B1076

The production of butyric acid by pHp?wd=%28Clostridium butyricum%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>Clostridium butyricum ZJUCB at various pH values was investigated. In order to study the effect of pH on cell growth, butyric acid biosynthesis and reducing sugar consumption, different cultivation pH values ranging from 6.0 to 7.5 were evaluated in 5-L bioreactor. In controlled pH pHp?wd=%28batch fermentation%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>batch fermentation, the optimum pH for cell growth and butyric acid production was 6.5 with a cell yield of 3.65 g/L and butyric acid yield of 12.25 g/L. Based on these results, this study then compared batch and fed-pHp?wd=%28batch fermentation%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>batch fermentation of butyric acid production at pH 6.5. Maximum value (16.74 g/L) of butyric acid concentration was obtained in fed-pHp?wd=%28batch fermentation%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>batch fermentation compared to 12.25 g/L in pHp?wd=%28batch fermentation%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>batch fermentation. It was concluded that cultivation under fed-pHp?wd=%28batch fermentation%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>batch fermentation mode could enhance butyric acid production significantly (P<0.01) by C. butyricum ZJUCB.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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