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CLC number: Q949.32

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Received: 2005-08-18

Revision Accepted: 2005-10-21

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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.1 P.28~33

http://doi.org/10.1631/jzus.2006.B0028


Promoter trapping in Magnaporthe grisea


Author(s):  Liu Xiao-hong, Lu Jian-ping, Wang Jiao-yu, Min Hang, Lin Fu-cheng

Affiliation(s):  Biotechnology Institute, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   minhang@zju.edu.cn, fuchenglin@zju.edu.cn

Key Words:  Promoter trapping, Green fluorescent protein, Magnaporthe grisea


Liu Xiao-hong, Lu Jian-ping, Wang Jiao-yu, Min Hang, Lin Fu-cheng. Promoter trapping in Magnaporthe grisea[J]. Journal of Zhejiang University Science B, 2006, 7(1): 28~33.

@article{title="Promoter trapping in Magnaporthe grisea",
author="Liu Xiao-hong, Lu Jian-ping, Wang Jiao-yu, Min Hang, Lin Fu-cheng",
journal="Journal of Zhejiang University Science B",
volume="7",
number="1",
pages="28~33",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0028"
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%0 Journal Article
%T Promoter trapping in Magnaporthe grisea
%A Liu Xiao-hong
%A Lu Jian-ping
%A Wang Jiao-yu
%A Min Hang
%A Lin Fu-cheng
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 1
%P 28~33
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0028

TY - JOUR
T1 - Promoter trapping in Magnaporthe grisea
A1 - Liu Xiao-hong
A1 - Lu Jian-ping
A1 - Wang Jiao-yu
A1 - Min Hang
A1 - Lin Fu-cheng
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 1
SP - 28
EP - 33
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0028


Abstract: 
Application of promoter trapping based on transformation in Magnaporthe grisea is reported in this paper. Two promoter-trapping vectors, designated as pCBGFP and pEGFPHPH, were constructed and transformed into protoplasts of M. grisea. A library of 1077 transformants resistant to hygromycin B was generated. Of which, 448 transformants were found to express eGFP gene in different structures of M. grisea. Three transformants grew slowly, 5 transformants decreased in conidiation and 7 transformants reduced in pathogenicity greatly among these 448 transformants. Eleven transformants were checked by genomic southern blot randomly, and 9 of which were single-copy insertions. The promoter trapping technique has been applied successfully in M. grisea and can be used as a tool for functional genomic analysis.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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