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CLC number: Q949.95

On-line Access: 2011-05-06

Received: 2010-05-16

Revision Accepted: 2010-11-15

Crosschecked: 2011-04-12

Cited: 2

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.5 P.393-398


Molecular authentication of geo-authentic Scrophularia ningpoensis

Author(s):  Chuan Chen, Li-na Duan, Xiao-long Zhou, Bing-long Chen, Cheng-xin Fu

Affiliation(s):  Key Laboratory of Conservation Biology for Endangered Wildlife of Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, China, Laboratory of Systematic and Evolutionary Botany and Biodiversity, Institute of Plant Sciences and Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, Hangzhou 310058, China, Pan’an Institute of Traditional Chinese Medicine, Pan’an 322300, China

Corresponding email(s):   cxfu@zju.edu.cn

Key Words:  Inter-simple sequence repeat (ISSR), Sequence characterized amplified region (SCAR), Scrophularia ningpoensis, Chinese Materia Medica, Traditional Chinese medicine

Chuan Chen, Li-na Duan, Xiao-long Zhou, Bing-long Chen, Cheng-xin Fu. Molecular authentication of geo-authentic Scrophularia ningpoensis[J]. Journal of Zhejiang University Science B, 2011, 12(5): 393-398.

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author="Chuan Chen, Li-na Duan, Xiao-long Zhou, Bing-long Chen, Cheng-xin Fu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Molecular authentication of geo-authentic Scrophularia ningpoensis
%A Chuan Chen
%A Li-na Duan
%A Xiao-long Zhou
%A Bing-long Chen
%A Cheng-xin Fu
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 5
%P 393-398
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000179

T1 - Molecular authentication of geo-authentic Scrophularia ningpoensis
A1 - Chuan Chen
A1 - Li-na Duan
A1 - Xiao-long Zhou
A1 - Bing-long Chen
A1 - Cheng-xin Fu
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 5
SP - 393
EP - 398
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000179

Scrophularia ningpoensis has long been used in the chinese Materia Medica for inflammation. Like other herbal medicines, S. ningpoensis collected from different localities may considerably differ in their therapeutic efficacy, and the one grown in Zhejiang Province is recognized as geo-authentic. However, it is difficult to confirm the geographical authenticity by similar morphological characteristics. In the present study, inter-simple sequence repeat (ISSR) markers were conducted to detect S. ningpoensis from different origins. A 1 259-bp fragment amplified by primer UBC874 was found only in geo-authentic ones. By cloning and sequencing that specific band, sequence characterized amplified region (SCAR) markers were designed to distinguish geo-authentic S. ningpoensis from others. This is a rapid and easy method that can be used to identify the geographical authenticity of S. ningpoensis.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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